苯肼诱发昆明种小鼠溶血性贫血模型的建立

发布时间：2018-10-12 08:11

【摘要】：目的观察昆明种小鼠腹腔注射苯肼后外周血成熟红细胞和网织红细胞的变化规律,建立昆明种小鼠溶血性贫血模型。方法健康雄性昆明种小鼠18只,随机分为溶剂对照组、低剂量苯肼组和高剂量苯肼组,每组6只。对照组于第0天经腹腔注射生理盐水,低剂量苯肼组和高剂量苯肼组动物分别经腹腔一次注射30和60 mg/kg·bw的苯肼,之后每天鼠尾采血进行红细胞计数和网织红细胞计数。结果苯肼注射后,低剂量苯肼组和高剂量苯肼组小鼠外周血红细胞总数与成熟红细胞总数均逐渐下降,分别在第5天和第4天达到最低,且高剂量苯肼组下降的程度显著高于低剂量苯肼组;此后,红细胞总数与成熟红细胞总数逐渐开始回升,在第13天恢复至对照组水平。同时,苯肼注射后,从第1天开始小鼠外周血网织红细胞总数和比例进行性上升,低剂量组网织红细胞总数和比例均于第6天达到最高,高剂量组网织红细胞总数于第5天达到最高,网织红细胞比例于第4天达到最高,之后便逐渐回落,于第13天完全恢复至正常水平。结论单次腹腔注射苯肼可诱发昆明小鼠轻度溶血性贫血,并刺激造血组织红系造血以恢复外周血红细胞水平,检测外周血红细胞总数和网织红细胞总数可以反映该溶血性贫血模型的贫血进展过程和红系造血功能状态。
[Abstract]:Objective to observe the changes of mature erythrocytes and reticulocytes in peripheral blood of Kunming mice after intraperitoneal injection of phenylhydrazine and to establish hemolytic anemia model of Kunming mice. Methods 18 healthy male Kunming mice were randomly divided into three groups: solvent control group, low dose phenylhydrazine group and high dose phenylhydrazine group. Rats in the control group were intraperitoneally injected with normal saline on the 0 th day. The animals in the low dose phenylhydrazine group and the high dose phenylhydrazine group were intraperitoneally injected with phenylhydrazine for 30 and 60 mg/kg bw, respectively. After that, erythrocyte count and reticulocyte count were collected from the tail of the rat every day. Results after the injection of phenylhydrazine, the total number of erythrocytes in peripheral blood and mature erythrocytes in the low dose phenylhydrazine group and the high dose phenylhydrazine group decreased gradually, and reached the lowest on the 5th and 4th day, respectively. The decrease of high dose phenylhydrazine group was significantly higher than that of low dose phenylhydrazine group, and after that, the erythrocyte count and mature erythrocyte count began to rise gradually, and returned to the control level on the 13th day. At the same time, the total number and proportion of reticulocyte in peripheral blood of mice gradually increased from the first day after injection of phenylhydrazine, and the total and proportion of reticulocyte in low dose group reached the highest level on the 6th day. The total number of high dose reticulocyte reached the highest on the 5th day, the reticulocyte ratio reached the highest on the 4th day, then gradually fell back to normal level on the 13th day. Conclusion A single intraperitoneal injection of phenylhydrazine can induce mild hemolytic anemia in Kunming mice and stimulate hematopoietic tissue erythropoiesis in order to restore the level of peripheral blood red blood cells. The total number of red blood cells and reticulocytes in peripheral blood can reflect the progression of anemia and the hemopoietic function of the hemolytic anemia model.
【作者单位】：北京航空航天大学生物与医学工程学院;
【基金】：国家自然科学基金(81573192;81072325)
【分类号】：R-332;R556

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