大鼠虚寒证模型的建立、评价及附子对虚寒证大鼠影响的研究

发布时间：2018-10-12 19:11

【摘要】：目的:探索寒性药物复制大鼠虚寒证模型的造模及模型评价方法。通过观察热性中药附子对虚寒证模型大鼠能量代谢及相关指标的影响,分析附子作用趋势、特点及规律,探寻反映实验动物寒热属性的客观指标,揭示其与寒热药物属性的内在联系,为更好的指导临床用药奠定基础。方法:用生石膏、龙胆草、黄柏和知母水煎液灌胃14天复制虚寒证大鼠模型,给予附子水煎液灌胃治疗7天,观察虚寒模型大鼠的一般状况、体温、寒热趋向等的变化;应用代谢笼收集大鼠24小时食量、饮水量、尿量和粪量;应用氧弹热量计检测大鼠摄入能、消化能、粪便能、可代谢能;采用酶联免疫吸附法检测大鼠血浆T3、T4、TSH、TRH含量;检测空腹血糖含量;检测肝组织糖原、乳酸含量、ATP酶活力、乳酸脱氢酶活力(LDH)、琥珀酸脱氢酶(SDH)活力;采用高效液相色谱法检测肝组织ATP含量及肝细胞能荷。结果:造模结束时模型组动物毛色污浊,腹泻明显,体重、自主活动及趾温明显降低,血浆丙酮酸、T3含量呈下降趋势。附子可以显著升高虚寒模型大鼠血浆T4含量及肝乳酸含量,明显提升肝乳酸脱氢酶和肝Na~+-K~+-ATP酶活力,显著升高肝细胞能荷;并且空腹血糖、体重、肛温、饮水量、尿量、在热箱的次数呈现一定的降低趋势;粪重、在寒箱的次数、单位体重摄入能、粪便能、单位体重粪便能,肝琥珀酸脱氢酶活力,ATP含量等指标呈现一定的升高趋势。结论:寒性药物生石膏、龙胆草、黄柏和知母配伍可成功复制大鼠虚寒证模型。热性中药附子能使虚寒模型大鼠能量代谢增强,改善大鼠虚寒状态,其药理作用的发挥同提高肝脏能量代谢和促进甲状腺激素的分泌关系密切。体重、体温、血浆甲状腺激素的含量、肝乳酸含量、ATP酶的活力、肝细胞能荷及空腹血糖含量等指标可以作为寒热证模型以及寒热性中药研究的重要评价指标。
[Abstract]:Objective: to explore the model and evaluation method of the model of asthenia cold syndrome in rats induced by cold drugs. By observing the effect of aconite on energy metabolism and related indexes in rats with deficiency and cold syndrome, the effect trend, characteristics and rules of aconite were analyzed, and the objective indexes reflecting the cold and heat properties of experimental animals were explored. To reveal the internal relationship between the drug properties of cold and heat, and to lay a foundation for better guidance of clinical drug use. Methods: the rat model of deficiency cold syndrome was established by oral administration of raw gypsum, gentian grass, Phellodendron chinensis and Anemarrhena chinensis for 14 days. The rats were treated with water decoction of aconite for 7 days. The changes of general condition, body temperature, cold and heat tendency of rats with deficiency cold were observed. The intake energy, digestibility energy, fecal energy and metabolizable energy of rats were measured by oxygen bomb calorimeter, and the contents of T3T4 TSHTSHTRH in plasma were detected by enzyme-linked immunosorbent assay (Elisa). Fasting blood glucose content, liver glycogen, lactic acid content, ATP enzyme activity, lactate dehydrogenase activity, (LDH), succinate dehydrogenase (SDH) activity, liver tissue ATP content and hepatocyte energy charge were detected by HPLC. Results: at the end of the model making, the model group showed a marked decrease in hair color, diarrhea, body weight, spontaneous activity and toe temperature, and the contents of plasma pyruvate and T3 decreased. Aconite could significantly increase plasma T4 content and liver lactate content, increase the activity of liver lactate dehydrogenase and liver Na~ K- ATP enzyme, and increase hepatic energy charge, fasting blood glucose, body weight, anus temperature, drinking water and urine volume. In the hot box, the number of fecal weight, the number of intake energy per unit body weight, fecal energy, succinate dehydrogenase activity and ATP content showed a certain increasing trend. Conclusion: the cold drug gypsum, gentian grass, Phellodendron chinensis and Anemarrhena can be successfully used to replicate the model of deficiency cold syndrome in rats. Radix aconite can enhance the energy metabolism and improve the cold state in rats with deficiency cold. Its pharmacological effect is closely related to the enhancement of liver energy metabolism and the promotion of thyroid hormone secretion. Body weight, body temperature, plasma thyroid hormone content, liver lactic acid content, ATP enzyme activity, liver cell energy charge and fasting blood glucose content can be used as important evaluation indexes of cold and heat syndrome model and research of cold and heat Chinese medicine.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332

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