先天性心脏病斑马鱼模型转录组测序分析
发布时间：2018-10-15 12:38
【摘要】：先天性心脏病是由于胎儿时期心脏和血管结构发育异常而导致的出生缺陷,其致病机制比较复杂。斑马鱼是目前生物学研究中应用较为广泛的模式动物。由于其胚胎透明、发育速度快等特点,有利于进行先天性心脏病研究。斑马鱼cloche突变型具有心内膜缺失和心脏发育畸形的突变表型,但依然可以存活,是研究先天性心脏病的良好动物模型。转录组测序分析能够从整体水平研究基因表达。对发育30hpf时期野生型斑马鱼和突变型cloche斑马鱼进行转录组测序,分析基因表达情况,对差异表达基因分析所涉及的相关生物学过程和通路,从总体上揭示cloche突变伴随的相关生物学过程和通路所发生的改变。分析发现新的转录本和转录区域,对斑马鱼基因组注释信息的完善提供新的信息。差异表达的新转录本可为cloche突变机制研究提供更多新的线索。重点分析cloche13号染色体末端的缺失区域,对缺失区域范围进行界定,分析缺失区域基因表达情况,对缺失区域内差异表达基因特别是lycat基因所在通路基因和互作基因的差异表达情况进行分析,揭示cloche缺失区域基因缺失表达后所引起的其它基因表达变化情况。
[Abstract]:Congenital heart disease (CHD) is a birth defect caused by abnormal development of heart and blood vessels during fetal period. Zebrafish is a widely used model animal in biological research. Because of its transparent embryo, rapid development and other characteristics, is conducive to the study of congenital heart disease. Zebrafish cloche mutant has the mutant phenotype of endocardial deletion and cardiac deformity, but it can still survive. It is a good animal model to study congenital heart disease. Transcriptome sequencing can be used to study gene expression at the global level. The transcriptome sequencing of wild zebrafish and mutant cloche zebrafish during developmental 30hpf period was carried out to analyze the gene expression and the related biological processes and pathways involved in differential expression gene analysis. The related biological processes and pathways associated with cloche mutation were revealed in general. New transcripts and transcriptional regions were found to provide new information for the improvement of zebrafish genome annotation information. The new transcripts of differential expression may provide more new clues for the study of cloche mutation mechanism. The region of deletion at the end of chromosome cloche13 was analyzed, the region of deletion was defined, and the expression of gene in deletion region was analyzed. The differential expression of differentially expressed genes, especially the genes involved in the pathway of lycat gene and the interaction genes in the deletion region, was analyzed to reveal the changes of other genes expression caused by the gene deletion in the cloche deletion region.
【学位授予单位】：中国科学院北京基因组研究所
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R541.1;R-332
本文编号：2272581
[Abstract]:Congenital heart disease (CHD) is a birth defect caused by abnormal development of heart and blood vessels during fetal period. Zebrafish is a widely used model animal in biological research. Because of its transparent embryo, rapid development and other characteristics, is conducive to the study of congenital heart disease. Zebrafish cloche mutant has the mutant phenotype of endocardial deletion and cardiac deformity, but it can still survive. It is a good animal model to study congenital heart disease. Transcriptome sequencing can be used to study gene expression at the global level. The transcriptome sequencing of wild zebrafish and mutant cloche zebrafish during developmental 30hpf period was carried out to analyze the gene expression and the related biological processes and pathways involved in differential expression gene analysis. The related biological processes and pathways associated with cloche mutation were revealed in general. New transcripts and transcriptional regions were found to provide new information for the improvement of zebrafish genome annotation information. The new transcripts of differential expression may provide more new clues for the study of cloche mutation mechanism. The region of deletion at the end of chromosome cloche13 was analyzed, the region of deletion was defined, and the expression of gene in deletion region was analyzed. The differential expression of differentially expressed genes, especially the genes involved in the pathway of lycat gene and the interaction genes in the deletion region, was analyzed to reveal the changes of other genes expression caused by the gene deletion in the cloche deletion region.
【学位授予单位】：中国科学院北京基因组研究所
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R541.1;R-332
【参考文献】
相关期刊论文 前2条
1 马宁;霍中军;颜广;黄红辉;罗深秋;张文清;;一种新的斑马鱼cloche突变体亚型的基因鉴定[J];南方医科大学学报;2010年03期
2 宫立国,邱广蓉,姜辉,徐小延,朱宏玉,孙开来;单纯性先天性心脏病易感区域12q13内HOXC簇基因单核苷酸多态单倍型分析(英文)[J];中华医学遗传学杂志;2005年05期
，本文编号：2272581
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