中药雾化吸入及鼻腔中药灌洗法对鼻咽癌动物模型的影响

发布时间：2018-10-18 09:45

【摘要】：目的:探究中药雾化吸入及鼻腔中药灌洗法给药对鼻咽癌放疗后鼻腔、鼻咽组织的修复作用,为临床医生选择给药途径提供理论依据。方法:将80只豚鼠分为对照组、单纯照射组、鼻腔中药灌洗组、中药雾化吸入组,每组20只。除对照组外的豚鼠无麻醉下固定器固定,用Co-60治疗机产生的射线作鼻部照射,照射剂量30Gy,每次300c Gy,每周5次,共照射10次。照射开始当天即开始给药,鼻腔中药灌洗组用桂林医学院药学院提取的中药灌洗液对豚鼠鼻腔每天灌洗1次,共灌洗4周;中药雾化吸入组用桂林医学院药学院提取的中药雾化吸入液对豚鼠雾化吸入,每天1次,共雾化吸入4周;对照组及单纯照射组不用药。采集照射后第1天、2周、4周、8周、12周颈总动脉处血液行肝肾功能检测,同时取鼻咽部黏膜HE染色后行光学显微镜观察细胞形态,原位免疫组织化学法(TUNEL法)检测药物对黏膜组织内细胞凋亡的影响。结果:肝肾功能检测发现对照组、单纯照射组、鼻腔中药灌洗组及中药雾化吸入组ALT、AST、AST/ALT、BUN、Cr水平无统计学差异(P0.05);HE染色发现除对照组外,其余3组均呈不同程度的炎症状态,单纯照射组HE染色显示炎症细胞数目最多,而鼻腔中药灌洗组最少;采用TUNEL法检测药物对黏膜组织内细胞凋亡的影响,发现除对照组外均出现TUNEL阳性细胞,其中鼻腔中药灌洗组TUNEL阳性细胞数最少,其次为中药雾化吸入组。结论:鼻腔灌洗法给药途径较中药雾化吸入法治疗对于鼻咽癌放疗后损伤的鼻黏膜修复作用更佳,能够促进炎症的消退,抑制细菌的生长。
[Abstract]:Objective: to investigate the effect of Chinese medicine atomization inhalation and nasal Chinese medicine lavage on the repair of nasopharyngeal carcinoma (NPC) after radiotherapy, and to provide a theoretical basis for clinicians to choose the route of administration. Methods: 80 guinea pigs were divided into control group, simple irradiation group, nasal cavity Chinese medicine lavage group, Chinese medicine atomization inhalation group, 20 guinea pigs in each group. Except for the control group, the guinea pigs were fixed without anesthesia. The irradiation dose was 30 Gy with the irradiation dose of 30 Gy, 5 times a week with 300c Gy, 10 times a week. The nasal cavity Chinese medicine lavage group was perfused once a day for 4 weeks with the Chinese medicine lavage liquid extracted from the college of medicine of Guilin Medical College. The Chinese medicine atomization inhalation group used the Chinese medicine atomization inhalation liquid extracted from Guilin Medical College of Pharmacology to the guinea pig atomization inhalation once a day for a total of 4 weeks; the control group and the simple irradiation group did not use drugs. The liver and kidney function were measured at the common carotid artery on day 1, day 2, week 4, week 8 and week 12 after irradiation. At the same time, the nasopharyngeal mucosa was stained with HE and the morphology of the cells was observed by optical microscope. In situ immunohistochemical method (TUNEL) was used to detect the effect of drugs on apoptosis in mucosal tissue. Results: the levels of ALT,AST,AST/ALT,BUN,Cr in the control group, the irradiation group, the nasal cavity Chinese medicine lavage group and the Chinese medicine atomization inhalation group were not significantly different from those in the control group (P0.05); HE staining showed that the other three groups showed different degree of inflammation except the control group. HE staining showed that the number of inflammatory cells was the highest in the irradiation group, while the number of the inflammatory cells was the least in the nasal cavity Chinese medicine lavage group, the effect of the drugs on the apoptosis of mucosal tissue was detected by TUNEL method, and TUNEL positive cells were found in all the cells except the control group. The number of TUNEL positive cells in nasal cavity Chinese medicine lavage group was the least, followed by traditional Chinese medicine atomization inhalation group. Conclusion: nasal lavage is more effective than traditional Chinese medicine atomization inhalation in repairing nasopharyngeal carcinoma (NPC) after radiotherapy. It can promote the regression of inflammation and inhibit the growth of bacteria.
【作者单位】：广西壮族自治区南溪山医院;广西中医药大学附属瑞康医院;
【基金】：广西壮族自治区科技厅课题:鼻咽鼻腔放疗后局部组织影响动物模型构建与中药局部干预及药效研究(桂科攻13349008)
【分类号】：R273;R-332

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