DMSO促进人胚胎干细胞体外造血分化

发布时间：2018-10-20 12:14

【摘要】：目的:探讨DMSO在人胚胎干细胞造血分化中的作用。方法:利用已建立的人胚胎干细胞逐级诱导分化体系,通过流式细胞术分析并结合免疫荧光测定研究DMSO在造血分化中的作用,同时通过在不同时间点添加DMSO,确定其发挥作用的窗口期。结果:免疫荧光及流式细胞术分析发现,在人胚胎干细胞造血分化过程中添加DMSO显著促进CD43~+造血祖细胞的产生;流式细胞术分析发现,DMSO显著促进APLNR+侧板中胚层细胞和CD31~+CD34~+生血内皮细胞的产生;在侧板中胚层细胞产生的窗口添加DMSO显著促进CD31~+CD34~+生血内皮细胞和CD43~+造血祖细胞的产生。结论:DMSO通过促进侧板中胚层的产生促进人胚胎干细胞造血分化能力,在侧板中胚层发生窗口添加DMSO能够显著促进造血祖细胞的产生。
[Abstract]:Objective: to investigate the role of DMSO in hematopoietic differentiation of human embryonic stem cells. Methods: the effect of DMSO on hematopoietic differentiation was studied by flow cytometry and immunofluorescence assay. At the same time, by adding DMSO, at different time points to determine the window period. Results: the results of immunofluorescence and flow cytometry showed that the addition of DMSO in the process of hematopoietic differentiation of human embryonic stem cells significantly promoted the production of CD43~ hematopoietic progenitor cells. Flow cytometry showed that DMSO significantly promoted the production of APLNR lateral plate mesodermal cells and CD31~ CD34~ hematopoietic endothelial cells, and the addition of DMSO to the window of lateral plate mesoderm cells significantly promoted the production of CD31~ CD34~ hematopoietic endothelial cells and CD43~ hematopoietic progenitor cells. Conclusion: DMSO can promote the hematopoietic differentiation of human embryonic stem cells by promoting the generation of mesoderm of lateral plate, and the addition of DMSO to the window of mesoderm formation can significantly promote the generation of hematopoietic progenitor cells.
【作者单位】：中国医学科学院北京协和医学院血液病医院(血液学研究所)实验血液学国家重点实验室;
【基金】：国家自然科学基金(31500949) 中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-018,2016-I2M-3-002)
【分类号】：R329.2

【相似文献】