阴虚血瘀型宫环出血病病证结合大鼠模型的建立及研究

发布时间：2018-10-22 19:01

【摘要】：目的：本实验旨在中医理论指导下,探讨复制宫环出血病阴虚血瘀证动物模型的方法,并对其发病机理进行研究。方法：本文回顾了中西医关于阴虚血瘀型宫环出血病的研究现状和进展,将大鼠按随机数字表分为四组：空白组、置环组、模型组、治疗组。通过手术置环结合药物灌胃的复合手法构建宫环出血病阴虚血瘀型大鼠模型,观察各组大鼠一般行为改变、体重变化以及大鼠子宫形态学改变,并通过治疗组对比观察,最后利用蛋白质抗体芯片对大鼠子宫组织中细胞因子表达水平进行检测,观察各组中细胞因子变化情况,通过药物对细胞因子的干预情况来进一步佐证动物模型。结果：模型组大鼠行为异常,主要表现为饮食饮水增加、活动度增加,兴奋性提高,易激怒,毛色失去光泽,生长较对照组缓慢,其差异具有高度统计学意义(P0.01)。子宫组织形态涂片呈现内膜上皮细胞坏死脱落,炎症细胞浸润等。与置环组相比,模型组中白介素-1β(IL-1β)、肿瘤细胞坏死因子-α(TNF-α)、单核细胞趋化蛋白-1(MPC-1)以及基质金属蛋白酶组织抑制因子(TIMP-1)4种细胞因子的表达水平发生了显著变化。其中IL-1β、TNF-α水平同空白组相比升高,其差异具有高度统计学意义(P0.01),MCP-1较空白组显著升高,其差异具有统计学意义(P0.05), TIMP-1较空白组显著降低,其差异具有统计学意义(P0.05)。结论：实验证明置环结合药物灌胃的复合式造模方法比单一的置环能更显著的影响大鼠体重、细胞形态学以及组织内细胞因子表达水平。药物治疗佐证了模型的合理性。蛋白质抗体芯片能平行的检测多种细胞因子的表达水平,初步估计IL-1β、TNF-α、MPC-1以及TIMP-1四种细胞因子与阴虚血瘀有关,但仍需大量实验证明。
[Abstract]:Objective: under the guidance of traditional Chinese medicine (TCM) theory, the aim of this experiment was to explore the method of replicating the animal model of uterine ring hemorrhage with yin deficiency and blood stasis, and to study its pathogenesis. Methods: the present situation and progress of traditional Chinese and western medicine on uterine ring haemorrhage of yin deficiency and blood stasis type were reviewed. Rats were randomly divided into four groups: blank group, ring setting group, model group and treatment group. A rat model of uterine ring hemorrhage with yin deficiency and blood stasis was established by the combined manipulation of surgical ring placement and drug administration. The changes of general behavior, body weight and uterine morphology of rats in each group were observed and compared with those in the treatment group. Finally, the expression level of cytokines in rat uterus was detected by protein antibody chip, and the changes of cytokines in each group were observed, and the animal model was further confirmed by the intervention of drugs on cytokines. Results: the rats in the model group showed abnormal behavior, mainly manifested by the increase of diet and drinking water, the increase of activity, the increase of excitability, the irritation, the loss of luster and the slower growth of the model group than those in the control group (P0.01). Uterine tissue morphologic smears showed necrosis and exfoliation of endometrial epithelial cells and infiltration of inflammatory cells. The expression levels of interleukin-1 尾 (IL-1 尾), tumor necrosis factor- 伪 (TNF- 伪), monocyte chemoattractant protein-1 (MPC-1) and tissue inhibitor of matrix metalloproteinase (TIMP-1) in the model group changed significantly compared with those in the annular group. The levels of IL-1 尾 and TNF- 伪 increased compared with the blank group, the difference was statistically significant (P0.01), MCP-1 was significantly higher than the blank group, the difference was statistically significant (P0.05), the TIMP-1 was significantly lower than the blank group, the difference was statistically significant (P0.05). Conclusion: the experimental results showed that the combined method of intragastric perfusion with ring insertion could significantly affect the body weight, cell morphology and cytokine expression level of rats. Drug therapy proved the rationality of the model. Protein antibody chip could detect the expression level of many cytokines in parallel. It was estimated that four cytokines, IL-1 尾, TNF- 伪, MPC-1 and TIMP-1, were related to blood stasis due to yin deficiency, but a large number of experiments were needed to prove them.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R271.9

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