308nm准分子光对白癜风豚鼠模型Treg和Th17细胞的影响

发布时间：2018-10-29 16:25

【摘要】：目的:通过白癜风豚鼠模型,观察308nm准分子光的疗效及对Treg、Thl7细胞的影响,探讨紫外光疗机制中的免疫学通路。方法:通过文件检索使用化学脱色法制备白癜风豚鼠模型并筛选造模成功的小鼠作为实验对象,将小鼠分为实验组和对照组,另选取相同数量的健康小鼠作为空白组,对实验组进行308nm准分子光的干预治疗,造模成功的对照组以及健康小鼠的空白组均不予以处理,10周后分别从肉眼复色程度、组织病理及免疫组化结果中的改变、mRNA以及蛋白表达的水平观察IL-17细胞和Treg细胞在三组之间的实验过程中的变化趋势。结果:308准分子光对白癜风豚鼠模型达到治疗效果,经过10周治疗后,5只豚鼠达到完全复色。在组织病理中,白癜风模型组豚鼠皮损中表皮黑素细胞减少,伴随真皮淋巴细胞浸润。治疗后,从组织学水平观察到,黑素细胞的增多,以及淋巴细胞浸润减少。免疫组织化学染色对皮损中IL-17标记可见,造模成功组中真皮层可见阳性结果的出现,此模型可用于本课题的研究。通过对皮损中IL-17及Foxp3的mRNA及蛋白表达水平定量检测发现,模型组IL-17表达升高,Foxp3表达降低,治疗后IL-17表达水平降低,Foxp3表达水平升高。结论:308nm准分子光疗可以通过降低豚鼠白癜风皮损中Th17细胞水平,升高Treg细胞水平调节皮损中Th17和Treg细胞的平衡紊乱。
[Abstract]:Aim: to observe the effect of 308nm excimer light and its effect on Treg,Thl7 cells in vitiligo guinea pig model, and to explore the immunological pathway in the mechanism of ultraviolet light therapy. Methods: the guinea pig model of vitiligo was made by chemical decolorization method. The mice were divided into experimental group and control group, and the same number of healthy mice were selected as blank group. The experimental group was treated with 308nm excimer light. The successful control group and the blank group of healthy mice were not treated. After 10 weeks, the degree of color recovery and the changes of histological and immunohistochemical results were observed. The levels of mRNA and protein expression were used to observe the changes of IL-17 cells and Treg cells in the three groups. Results: 308 excimer light was effective in treating vitiligo guinea pigs. After 10 weeks of treatment, 5 guinea pigs were completely multicolored. In histopathology, epidermal melanocytes in the lesions of guinea pigs with vitiligo model were decreased, accompanied by dermal lymphocyte infiltration. After treatment, the increase of melanocytes and the decrease of lymphocyte infiltration were observed at histological level. Immunohistochemical staining can be used to detect IL-17 markers in skin lesions and positive results in dermis in the successful model group. This model can be used in the study of this subject. The expression levels of mRNA and protein in IL-17 and Foxp3 were detected by quantitative analysis. The results showed that the expression of IL-17 and Foxp3 in model group was higher than that in control group. After treatment, the expression of IL-17 and Foxp3 were decreased. Conclusion: 308nm excimer phototherapy can regulate the imbalance of Th17 and Treg cells by decreasing the level of Th17 cells in the lesions of vitiligo and increasing the level of Treg cells.
【作者单位】：上海交通大学附属第六人民医院皮肤科;
【分类号】：R758.41;R-332

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