贵州省3个少数民族线粒体DNA 9 bp序列缺失频率的分析研究

发布时间：2018-11-01 16:25

【摘要】：目的探讨贵州省3个少数民族线粒体DNA(mtDNA)9bp序列缺失频率情况。方法随机选取贵州省雷山县苗族(69例)、荔波县布依族(70例)、荔波县水族(44例)共183例男性血液标本,应用PCR及直接测序检测线粒体DNA 9bp序列缺失情况。结果在贵州省3个少数民族样本中发现标准型、缺失型、3型,缺失频率最高为荔波县水族(40.91%),雷山县苗族中检出3型1例,3个少数民族群体间mtDNA 9bp缺失频率比较差异无统计学意义(P0.05)。结论 3个世居少数民族mtDNA 9bp缺失频率不同,水族的遗传变异较大,水族与苗族的亲缘关系相比水族与布依族较近。
[Abstract]:Objective to investigate the frequency of mitochondrial DNA (mtDNA) 9bp deletion in three minority nationalities in Guizhou province. Methods A total of 183 male blood samples were randomly selected from Miao nationality (69 cases), Buyi nationality (70 cases) and aquatic nationality (44 cases) in Leishan County, Guizhou Province. Mitochondrial DNA 9bp deletion was detected by PCR and direct sequencing. Results Standard type, deletion type and 3 type were found in 3 minority nationality samples in Guizhou province. The highest frequency of deletion was found in Shui nationality of Libo County (40.91%), and in one case of Miao nationality in Lishan County. There was no significant difference in the frequency of mtDNA 9bp deletion among the three ethnic groups (P0.05). Conclusion the frequency of mtDNA 9bp deletion in the three ethnic minorities is different, the genetic variation of the aquarium is greater, and the genetic relationship between the Shui and Miao is closer than that between the Shui and Buyi.
【作者单位】：地方病与少数民族性疾病教育部重点实验室/贵州医科大学分子生物学重点实验室;
【基金】：贵州省“2011协同创新中心”项目(黔教合协同创新中心[2014]06号) 贵州省科技厅基金资助项目(黔科合J字[2011]2119号)
【分类号】：R394

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