补肾助孕方对LPD模型大鼠垂体GnRH受体信号转导系统的影响

发布时间：2018-11-08 19:36

【摘要】：目的通过观察补肾助孕方对大鼠垂体促性腺激素及促性腺激素释放激素(GnRH)受体信号系统的影响,探究其作用机制,寻找中医心-肾-子宫轴理论的现代科学依据。方法采用米非司酮对SD雌鼠进行造模,并进行药物干预。采用ELISA法检测大鼠血清样本促黄体生成激素(LH)和促卵泡成熟激素(FSH)水平,qPCR法检测各组大鼠垂体FSHβ、LHβ基因转录水平。对大鼠垂体GnRH受体信号转导系统关键分子以及下游重要转录因子分别采用qPCR与Western blot方法检测其基因转录与蛋白表达水平。结果补肾助孕方显著提高了由米非司酮诱导的血清低FSH水平与垂体GnRH受体低表达(P0.05),补肾助孕方组高、低剂量组中PKC、p38MAPK、JNK、ERK、PKA等关键信号转导分子以及c-Jun、Elk-1、Egr-1、CREB等转录因子的mRNA及蛋白表达均显著高于模型组(P0.05),但CAM及其下游的Nur77在各组间均无显著差异。结论补肾助孕方能够通过调节垂体GnRH受体信号转导系统调节垂体促性腺激素的分泌水平,证明该方具有调节生殖轴整体功能的作用,阐释了中医心-肾-子宫轴理论与现代医学下丘脑-垂体-卵巢轴在内容上的一致性。
[Abstract]:Objective to investigate the effect of Bushen Zhuyun recipe (BZF) on pituitary gonadotropin and gonadotropin releasing hormone (GnRH) receptor signal system in rats, to explore its mechanism and to find out the modern scientific basis of the theory of heart-kidney uterus axis of traditional Chinese medicine. Methods Mifepristone was used to model SD female mice and drug intervention was carried out. The levels of luteinizing hormone (LH) and follicle maturation hormone (FSH) in serum samples of rats were detected by ELISA method, and the transcription levels of FSH 尾 and LH 尾 genes in pituitary gland of rats in each group were detected by qPCR method. The gene transcription and protein expression level of rat pituitary GnRH receptor signal transduction system and downstream important transcription factors were detected by qPCR and Western blot respectively. Results Bushenzhuyong prescription significantly increased the serum low FSH level induced by mifepristone and the low expression of pituitary GnRH receptor (P0.05). The Bushen Zhuyong prescription group was high and low dose PKC,p38MAPK,JNK,ERK, group. The expression of mRNA and protein of key signal transduction molecules such as PKA and c-Jun-Elk-1Egr-1CREB were significantly higher than that of model group (P0.05), but there was no significant difference in CAM and its downstream Nur77 among the three groups. Conclusion BSZF can regulate the secretion of pituitary gonadotropin by regulating the signal transduction system of pituitary GnRH receptor, which proves that it has the function of regulating the whole function of reproductive axis. The consistency between the theory of heart-kidney-uterus axis of TCM and that of hypothalamus-pituitary-ovary axis in modern medicine is explained.
【作者单位】：南京中医药大学第一临床医学院;
【基金】：国家自然科学基金(81473713) 江苏省自然科学基金(BK20141463) 江苏省中医药管理局重点项目(ZD201702)
【分类号】：R285.5;R-332

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