肺部恶性肿瘤原代裸鼠移植瘤模型的建立
发布时间：2018-11-09 15:06
【摘要】：背景与目的动物模型是研究癌症的重要工具。本研究的目的是建立人肺部恶性肿瘤组织裸鼠移植瘤的成熟方法,并积累一定数量的动物模型,为肺部恶性肿瘤提供有效的研究平台。 方法将手术切除的肺部恶性肿瘤组织的新鲜标本移植于裸鼠皮下,观察移植瘤的生长情况,待皮下移植瘤长成后对其进行连续传代和组织学观察。选用裸鼠体内传代的移植瘤模型进行体内药效实验。 结果共移植101例人肺部恶性肿瘤的组织标本,其中93例术后病理证实为原发性非小细胞肺癌,3个月内成瘤27例(29%)；3例为肺转移癌,成瘤率为100%。各代移植瘤模型的组织病理学与人源肿瘤组织相一致。对1例原发于子宫内膜的肺转移癌裸鼠移植瘤模型进行了体内药效试验,结果显示紫杉醇、顺铂和吉西他滨能在不同程度上抑制裸鼠移植瘤的生长。 结论成功建立了肺部恶性肿瘤的裸鼠皮下移植瘤模型,并较好地保留了人源肿瘤的恶性特征和组织学特性,可为肺部恶性肿瘤的研究提供良好的实验平台。
[Abstract]:Background and objective Animal models are important tools for cancer research. The purpose of this study was to establish a mature method for transplanted human lung malignant tumors in nude mice and to accumulate a certain number of animal models to provide an effective platform for the study of lung malignant tumors. Methods fresh specimens of lung malignant tumor were transplanted into nude mice subcutaneously, the growth of transplanted tumor was observed, and the subcutaneous transplanted tumor was subcultured and histologically observed. The model of xenograft tumor in nude mice was used to carry out in vivo pharmacodynamics experiment. Results among 101 cases of lung cancer, 93 cases were confirmed as primary non-small cell lung cancer (NSCLC) after operation, 27 cases (29%) were transplanted within 3 months, and 3 cases were lung metastases, the rate of tumorigenesis was 100 cases. The histopathology of various generations of transplanted tumor models is consistent with that of human tumor tissues. A nude mouse model of lung metastatic carcinoma derived from endometrium was tested in vivo. The results showed that paclitaxel, cisplatin and gemcitabine could inhibit the growth of transplanted tumor in nude mice to some extent. Conclusion the subcutaneous transplanted tumor model of lung malignant tumor was successfully established in nude mice, and the malignant and histological characteristics of human tumor were well preserved, which could provide a good experimental platform for the study of lung malignant tumor.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R734.2
本文编号：2320758
[Abstract]:Background and objective Animal models are important tools for cancer research. The purpose of this study was to establish a mature method for transplanted human lung malignant tumors in nude mice and to accumulate a certain number of animal models to provide an effective platform for the study of lung malignant tumors. Methods fresh specimens of lung malignant tumor were transplanted into nude mice subcutaneously, the growth of transplanted tumor was observed, and the subcutaneous transplanted tumor was subcultured and histologically observed. The model of xenograft tumor in nude mice was used to carry out in vivo pharmacodynamics experiment. Results among 101 cases of lung cancer, 93 cases were confirmed as primary non-small cell lung cancer (NSCLC) after operation, 27 cases (29%) were transplanted within 3 months, and 3 cases were lung metastases, the rate of tumorigenesis was 100 cases. The histopathology of various generations of transplanted tumor models is consistent with that of human tumor tissues. A nude mouse model of lung metastatic carcinoma derived from endometrium was tested in vivo. The results showed that paclitaxel, cisplatin and gemcitabine could inhibit the growth of transplanted tumor in nude mice to some extent. Conclusion the subcutaneous transplanted tumor model of lung malignant tumor was successfully established in nude mice, and the malignant and histological characteristics of human tumor were well preserved, which could provide a good experimental platform for the study of lung malignant tumor.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R734.2
【参考文献】
相关期刊论文 前5条
1 吕慧;周清华;;动物模型在肺癌研究中的应用[J];中国肺癌杂志;2007年01期
2 卓莹;吴一龙;郭爱林;陈思远;苏健;;荷人肺癌小鼠皮下移植瘤模型的建立及其生物学特性初探[J];中国肺癌杂志;2010年06期
3 孙冰;吴世凯;宋三泰;;NOD/SCID小鼠平台上的肿瘤实验研究进展[J];中华肿瘤防治杂志;2008年04期
4 冯仕庭;孙灿辉;蔡华崧;周健;谭国胜;邱鹏新;张健;李子平;孟悛非;;建立荷人结肠癌裸鼠移植瘤模型及MRI成像检查[J];中国组织工程研究与临床康复;2010年20期
5 余琛琳;崔淑芳;;肺癌动物模型的制备与应用[J];中国实验动物学报;2008年06期
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