瘦素在大鼠心肌缺血—再灌注损伤中作用的研究
发布时间：2018-11-09 15:34
【摘要】：目的观察瘦素对心肌缺血-再灌注损伤(MIRI)大鼠模型的心肌影响,探讨瘦素在MIRI中的作用,阐明瘦素在心肌缺血-再灌注损伤的重要病理生理意义,并指导心肌缺血-再灌注损伤疾病的临床预防与治疗。 方法:24只雄性SD大鼠,按随机数字表法分为4组(n=6):①假手术(sham)组、②标准MIRI组、③瘦素预处理(Leptin+MIRI)组和④瘦素处理(MIRI+Leptin)组。③组大鼠于MIRI术前30 min予腹腔注射重组瘦素(美国RD Systems公司),剂量为50μg/kg。④组大鼠于MIRI手术后30 min予腹腔注射重组瘦素,剂量为50μg/kg。以上4组大鼠的标本均于MIRI术后120 min采集。观察心肌梗死面积、心肌瘦素水平以及血清瘦素、磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)、天冬氨酸转氨酶(AST)、肌钙蛋白(TnT)水平。 结果瘦素处理组心肌梗死面积〔(39.43±2.06)%〕、心肌瘦素水平〔(23.1±2.95)μg/g〕、血清瘦素水平〔(38.37±1.98)μg/g〕显著高于MIRI组心肌梗死面积〔(33.20±2.12)%〕、心肌瘦素水平〔(15.50±3.14)μg/g〕血清瘦素水平〔(29.49±2.01μg/g〕;瘦素预处理组心肌梗死面积〔(9.20±1.85)%〕、心肌瘦素水平〔(8.26±3.29)μg/g〕、血清瘦素水平〔(10.31±1.88)μg/g〕显著低于MIRI组心肌梗死面积〔(33.20±2.12)%〕、心肌瘦素水平〔(15.50±3.14)μg/g〕、血清瘦素水平〔(29.49±2.01)μg/g〕;瘦素处理组血清LDH〔(1067.4±123.2)U/L〕、血清AST〔(402.9±55.0)U/L〕、血清TnT浓度〔(0.98±0.13)μg/g〕显著高于MIRI组LDH〔(884.5±104.3)U/L〕、血清AST〔(327.1±44.9)U/L〕、血清TnT浓度〔(0.72±0.10)μg/g〕;瘦素预处理组血清CK〔(3231.7±640.1)U/L〕、LDH〔(610.4±86.0)U/L〕、血清AST〔(202.3±29.1)U/L〕、血清TnT浓度〔(0.51±0.09)μg/g〕显著低于MIRI组CK〔(5310.3±849.4)U/L〕、LDH〔(884.5±104.3)U/L〕、血清AST〔(327.1±44.9)U/L〕、血清TnT浓度〔(0.72±0.10)μg/g〕。 结论瘦素处理和高水平的瘦素加重了MIRI,瘦素预处理可通过减少心肌和血清的瘦素水平以减轻MIRI。
[Abstract]:Objective to observe the effects of leptin on myocardial ischemia reperfusion injury in (MIRI) rats, to explore the role of leptin in MIRI, and to elucidate the important pathophysiological significance of leptin in myocardial ischemia-reperfusion injury. To guide the clinical prevention and treatment of myocardial ischemia-reperfusion injury. Methods: Twenty-four male SD rats were randomly divided into 4 groups (n = 6): 1 sham operation (sham) group and 2 standard MIRI group. 3 leptin pretreated (Leptin MIRI) group and 4 leptin treated (MIRI Leptin) group. Three groups of rats were injected with recombinant leptin 30 min before MIRI (RD Systems Company, USA). The rats in the 50 渭 g/kg.4 group were intraperitoneally injected with recombinant leptin 30 min after MIRI operation, and the dose was 50 渭 g / kg. The specimens of the above four groups were collected at 120 min after MIRI. Myocardial infarction size, myocardial leptin level and serum leptin, (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST),) troponin (TnT) were measured. Results the myocardial infarction size and myocardial leptin level in leptin treated group were (39.43 卤2.06)% and (23.1 卤2.95) 渭 g / g, respectively. Serum leptin level (38.37 卤1.98 渭 g / g) was significantly higher than that in MIRI group (33.20 卤2.12%), myocardial leptin level (15.50 卤3.14) 渭 g / g, serum leptin level (29.49 卤2.01 渭 g / g); Myocardial infarction size and myocardial leptin level in leptin preconditioning group were (9.20 卤1.85)% and (8.26 卤3.29) 渭 g / g, respectively. Serum leptin level (10.31 卤1.88 渭 g / g) was significantly lower than that in MIRI group (33.20 卤2.12%), and myocardial leptin level was (15.50 卤3.14) 渭 g / g. Serum leptin level (29.49 卤2.01) 渭 g / g; Serum LDH (1067.4 卤123.2 U / L, serum AST (402.9 卤55.0 UP / L, serum TnT concentration (0.98 卤0.13) 渭 g / g in leptin treated group were significantly higher than LDH (884.5 卤104.3 UP / L in MIRI group. Serum AST (327.1 卤44.9 渭 g / L), serum TnT concentration (0.72 卤0.10) 渭 g / g; Leptin pretreatment group: serum CK (3231.7 卤640.1) L / L), LDH (610.4 卤86.0 U / L), serum AST (202.3 卤29.1) U / L), Serum TnT concentration (0.51 卤0.09) 渭 g / g) was significantly lower than that in MIRI group (CK (5310.3 卤849.4) UL), LDH (884.5 卤104.3 UP / L, serum AST (327.1 卤44.9 UP / L). Serum TnT concentration was (0.72 卤0.10) 渭 g / g. Conclusion leptin treatment and high levels of leptin increase MIRI, leptin preconditioning, which can reduce MIRI. by reducing serum and myocardial leptin levels.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363
本文编号：2320820
[Abstract]:Objective to observe the effects of leptin on myocardial ischemia reperfusion injury in (MIRI) rats, to explore the role of leptin in MIRI, and to elucidate the important pathophysiological significance of leptin in myocardial ischemia-reperfusion injury. To guide the clinical prevention and treatment of myocardial ischemia-reperfusion injury. Methods: Twenty-four male SD rats were randomly divided into 4 groups (n = 6): 1 sham operation (sham) group and 2 standard MIRI group. 3 leptin pretreated (Leptin MIRI) group and 4 leptin treated (MIRI Leptin) group. Three groups of rats were injected with recombinant leptin 30 min before MIRI (RD Systems Company, USA). The rats in the 50 渭 g/kg.4 group were intraperitoneally injected with recombinant leptin 30 min after MIRI operation, and the dose was 50 渭 g / kg. The specimens of the above four groups were collected at 120 min after MIRI. Myocardial infarction size, myocardial leptin level and serum leptin, (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST),) troponin (TnT) were measured. Results the myocardial infarction size and myocardial leptin level in leptin treated group were (39.43 卤2.06)% and (23.1 卤2.95) 渭 g / g, respectively. Serum leptin level (38.37 卤1.98 渭 g / g) was significantly higher than that in MIRI group (33.20 卤2.12%), myocardial leptin level (15.50 卤3.14) 渭 g / g, serum leptin level (29.49 卤2.01 渭 g / g); Myocardial infarction size and myocardial leptin level in leptin preconditioning group were (9.20 卤1.85)% and (8.26 卤3.29) 渭 g / g, respectively. Serum leptin level (10.31 卤1.88 渭 g / g) was significantly lower than that in MIRI group (33.20 卤2.12%), and myocardial leptin level was (15.50 卤3.14) 渭 g / g. Serum leptin level (29.49 卤2.01) 渭 g / g; Serum LDH (1067.4 卤123.2 U / L, serum AST (402.9 卤55.0 UP / L, serum TnT concentration (0.98 卤0.13) 渭 g / g in leptin treated group were significantly higher than LDH (884.5 卤104.3 UP / L in MIRI group. Serum AST (327.1 卤44.9 渭 g / L), serum TnT concentration (0.72 卤0.10) 渭 g / g; Leptin pretreatment group: serum CK (3231.7 卤640.1) L / L), LDH (610.4 卤86.0 U / L), serum AST (202.3 卤29.1) U / L), Serum TnT concentration (0.51 卤0.09) 渭 g / g) was significantly lower than that in MIRI group (CK (5310.3 卤849.4) UL), LDH (884.5 卤104.3 UP / L, serum AST (327.1 卤44.9 UP / L). Serum TnT concentration was (0.72 卤0.10) 渭 g / g. Conclusion leptin treatment and high levels of leptin increase MIRI, leptin preconditioning, which can reduce MIRI. by reducing serum and myocardial leptin levels.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363
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