补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响

发布时间：2018-11-13 07:55

【摘要】：目的:探讨补肾活血化痰汤对多囊卵巢综合征(PCOS)模型大鼠卵巢血供及形态的影响。方法:取SD大鼠(♀)50只随机分为正常对照组、模型组、阳性对照组[罗格列酮,3 mg/(kg·d)]和补肾活血化痰汤低、高剂量组[5.0、7.5 g/(kg·d)],每组10只。除正常对照组外,其余各组大鼠均采用Poresky法复制PCOS模型。成模后,各给药组大鼠ig相应药物,正常对照组和模型组大鼠ig生理盐水[10 m L/(kg·d)],每天1次,连续22 d。给药结束后处死大鼠,记录微血管密度(MVD),检测大鼠卵巢组织血管内皮生长因子(VEGF)、血管紧张素Ⅱ(AngⅡ)1型受体(AT1)和2型受体(AT2)以及G蛋白偶联受体MAS m RNA的表达,并观察卵巢形态变化。结果:与正常对照组比较,模型组和各给药组大鼠MVD均升高,AT1、AT2、MAS m RNA表达均下调(P0.05);模型组大鼠卵巢组织可见囊性窦卵泡、颗粒细胞层减少,卵母细胞消失,黄体组织数量减少等明显病理变化。与模型组比较,各给药组上述指标均得到显著改善,且补肾活血化痰汤低、高剂量组作用均强于阳性对照组(P0.05);各给药组大鼠的卵巢组织病理变化均得到一定改善。结论:补肾活血化痰汤可增加PCOS模型大鼠卵巢组织血供,促进血管生成,促排卵,有助于改善卵巢形态。
[Abstract]:Objective: to study the effect of Bushen Huoxue Huatan decoction on ovarian blood supply and morphology in polycystic ovary syndrome (PCOS) rats. Methods: fifty SD rats were randomly divided into normal control group, model group, positive control group [rosiglitazone, 3 mg/ (kg d)] and Bushen Huoxue Huatan decoction low, high dose group [5.0 ~ 7.5g / (kg d)]. 10 rats in each group. In addition to the normal control group, the other groups of rats using Poresky method to replicate the PCOS model. After the model was established, the corresponding drugs of ig were given to the rats in each group, and the normal saline [10 mL / (kg d)] of ig in the normal control group and the model group, once a day for 22 days. The rats were killed after administration and microvessel density (MVD),) was recorded to detect vascular endothelial growth factor (VEGF),) in ovarian tissue of rats. The expression of angiotensin 鈪，

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