旋毛虫病人血清Western blot对成虫可溶性蛋白中早期诊断抗原的鉴定
发布时间：2018-11-15 13:50
【摘要】：目的鉴定旋毛虫成虫早期特异性诊断抗原。方法应用感染19d和35d的旋毛虫病患者血清,通过Western blot对旋毛虫成虫可溶性蛋白的抗原组分进行筛选和鉴定。结果 SDS-PAGE显示,旋毛虫成虫可溶性蛋白有27条蛋白带,分子质量单位分别为95、81.3、73.4、63.6、61.4、55、50.6、47、45.1、43.7、41.9、40.6、39、37.4、35.1、33.3、31、30.4、27.8、26.6、25.4、24、21、19.2、17.9、15.4和14.4ku。Western blot显示,成虫可溶性蛋白中的41.9ku和50.6ku蛋白与感染19d和35d的旋毛虫病患者血清反应率均为100%(15/15),与囊尾蚴病、裂头蚴病、并殖吸虫病、华支睾吸虫病患者血清及健康人血清均无交叉反应,与棘球蚴病和日本血吸虫病患者血清的交叉反应率分别为23.8%和44.44%。结论旋毛虫成虫可溶性蛋白中的41.9ku和50.6ku蛋白用于人体旋毛虫病早期诊断具有高度的敏感性和良好的特异性。
[Abstract]:Objective to identify the early specific diagnostic antigen of Trichinella spiralis adult. Methods the antigen components of soluble protein of adult Trichinella spiralis were screened and identified by Western blot in sera of patients infected with trichinellosis for 19 and 35 days. Results SDS-PAGE showed that there were 27 protein bands in soluble protein of adult Trichinella spiralis. The molecular weight units are 95U 81.3C 73.4N 63.6N 61.4U 50.6U 45.6N 45.7U 41.9U 40.6N 41.9U 40.6N 39.39.36.31.31.31.31.31.31.31.31.31.33.3cu 33.826.625.42421,19.2hu 17.9 / 15.4, respectively, according to the results of 14.4ku.Western blot, the molecular weight units of the two groups are as follows: (1) the molecular weight units are:! 27. 826! 36.625. The response rates of 41.9ku and 50.6ku proteins in soluble proteins of adult worms were 100% (15 / 15) in sera of patients infected with trichinellosis for 19 and 35 days, and were associated with cysticercosis, cysticercosis and paragonimiasis. There was no cross reaction in sera of clonorchiasis patients and healthy persons. The cross reaction rates with sera of patients with echinococcosis and schistosomiasis japonicum were 23.8% and 44.44%, respectively. Conclusion 41.9ku and 50.6ku proteins in soluble protein of adult Trichinella spiralis have high sensitivity and good specificity for early diagnosis of human trichinellosis.
【作者单位】： 郑州大学医学院寄生虫学教研室;
【基金】：国家自然科学基金项目(No.81672043,81572024) 河南省高等学校科研项目(No.16A310017) 郑州大学大学生创新创业训练计划项目(No.2016xjxm326)
【分类号】：R383.15
本文编号：2333480
[Abstract]:Objective to identify the early specific diagnostic antigen of Trichinella spiralis adult. Methods the antigen components of soluble protein of adult Trichinella spiralis were screened and identified by Western blot in sera of patients infected with trichinellosis for 19 and 35 days. Results SDS-PAGE showed that there were 27 protein bands in soluble protein of adult Trichinella spiralis. The molecular weight units are 95U 81.3C 73.4N 63.6N 61.4U 50.6U 45.6N 45.7U 41.9U 40.6N 41.9U 40.6N 39.39.36.31.31.31.31.31.31.31.31.31.33.3cu 33.826.625.42421,19.2hu 17.9 / 15.4, respectively, according to the results of 14.4ku.Western blot, the molecular weight units of the two groups are as follows: (1) the molecular weight units are:! 27. 826! 36.625. The response rates of 41.9ku and 50.6ku proteins in soluble proteins of adult worms were 100% (15 / 15) in sera of patients infected with trichinellosis for 19 and 35 days, and were associated with cysticercosis, cysticercosis and paragonimiasis. There was no cross reaction in sera of clonorchiasis patients and healthy persons. The cross reaction rates with sera of patients with echinococcosis and schistosomiasis japonicum were 23.8% and 44.44%, respectively. Conclusion 41.9ku and 50.6ku proteins in soluble protein of adult Trichinella spiralis have high sensitivity and good specificity for early diagnosis of human trichinellosis.
【作者单位】： 郑州大学医学院寄生虫学教研室;
【基金】：国家自然科学基金项目(No.81672043,81572024) 河南省高等学校科研项目(No.16A310017) 郑州大学大学生创新创业训练计划项目(No.2016xjxm326)
【分类号】：R383.15
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