激素诱导下股骨头坏死兔模型血浆中三酰甘油代谢及中药干预作用的研究

发布时间：2018-11-18 17:06

【摘要】：目的:观察激素诱导下的股骨头坏死兔模型血浆中三酰甘油(TG)代谢特点及中药骨蚀灵胶囊的干预作用。方法:构建激素诱导下的股骨头坏死兔模型,运用UPLC/MS分析技术,观察兔模型血浆中TG体内代谢变化特点,分析与股骨头局部组织形态学改变的相关性;对比观察骨蚀灵胶囊对TG体内代谢的干预效果。结果:与正常对照组比较,模型组、中药组与西药组的TG代谢在各时间节点均出现显著紊乱(P0.01);与模型组比较,第3、5周时中药组与西药组的TG均下降(P0.05);中药与西药组间比较无显著差异;与模型组比较,中药组与西药组的MRI检查仅见少量高信号的关节腔积液影像及股骨头中心信号偏低,病理学显示股骨头骨坏死程度轻微,且中药组好于西药组。结论:中药骨蚀灵与西药辛伐他汀同样具有降低血脂中TG作用,这可能是中药骨蚀灵预防激素性股骨头坏死的作用机制之一;中药复方以其多"靶点",整合调节作用强,毒副作用小而更具优势。
[Abstract]:Aim: to observe the metabolic characteristics of triglyceride (TG) in plasma of steroid induced femoral head necrosis rabbit model and the intervention effect of Guyongling capsule. Methods: a steroid-induced rabbit model of femoral head necrosis was established. The metabolic characteristics of plasma TG in rabbits were observed by UPLC/MS analysis, and the correlation between the changes of local histomorphology and the changes of femoral head were analyzed. To observe the effect of Gukongling capsule on metabolism of TG. Results: compared with the normal control group, the TG metabolism of the model group, the traditional Chinese medicine group and the western medicine group were significantly disturbed at each time point (P0.01), and the TG of the traditional Chinese medicine group and the western medicine group decreased at the 3rd week (P0.05), compared with the model group (P0.01). There was no significant difference between the Chinese medicine group and the western medicine group. Compared with the model group, the MRI examination of the Chinese medicine group and the western medicine group showed only a small amount of hyperintense articular effusion image and the central signal of the femoral head were low, the pathology showed that the degree of osteonecrosis of the femoral head was slight, and the Chinese medicine group was better than the western medicine group. Conclusion: Guyongling has the same effect as simvastatin to reduce TG in blood lipids, which may be one of the mechanisms of Guyongling to prevent steroid-induced femoral head necrosis. Traditional Chinese medicine compound with its many "target", the integration regulation function is strong, the toxicity side effect is small and has the superiority.
【作者单位】：南昌大学第四附属医院;北京工业大学生命科学与生物工程学院;
【基金】：国家自然科学基金项目(编号:81473707) 江西省自然科学基金计划(编号:20151BAB205054) 江西省科技支撑计划项目(编号:20151BBG70202)
【分类号】：R285.5;R-332

【相似文献】