清肺培元颗粒对免疫低下肺部感染小鼠模型的影响

发布时间：2018-11-19 08:01

【摘要】：目的:探讨清肺培元颗粒对免疫低下肺部感染小鼠的可能作用机制。方法:建立免疫低下肺部感染小鼠模型,随机分为8组,各组给药14d后摘取眼球取静脉血;流式细胞术检测小鼠外周血中CD_4~+T、CD_8~+T细胞含量,计数CD_4~+/CD_8~+的比值;ELISA法测定小鼠血清中白细胞介素6(IL-6)及降钙素(PCT)含量。结果:各治疗组病理学改变较模型组轻,中西药组,清肺培元颗粒中、高剂量组改善明显;与正常对照组比较,模型组CD4+显著降低(P0.05),CD_8~+显著升高(P0.05);与模型组比较,用药后清肺培元颗粒3个剂量组小鼠,CD_4~+T淋巴细胞水平及CD_4~+/CD_8~+比值明显升高,同时降低CD_8~+T淋巴细胞数量(P0.05);模型小鼠血清中IL-6、PCT表达水平均较正常组增高(P0.05),各治疗组较模型组显著降低(P0.01)。结论:清肺培元颗粒有效地调节小鼠外周血T淋巴细胞亚群的水平,降低IL-6、PCT的表达水平,能够增强机体免疫功能。
[Abstract]:Objective: to investigate the possible mechanism of Qingfei Peiyuan granule on immunosuppression pulmonary infection mice. Methods: the model of immunosuppression pulmonary infection was established in mice. The mice were randomly divided into 8 groups. After 14 days of administration, the eyeballs were removed and the venous blood was taken out in each group. The content of CD8T cells and the ratio of CD_4~ / CD_8~ in peripheral blood of mice were measured by flow cytometry, and the contents of interleukin 6 (IL-6) and calcitonin (PCT) in serum of mice were measured by ELISA method. Results: the pathological changes of each treatment group were lighter than those of the model group, the high dose group of Qingfei Peiyuan granule and the traditional Chinese medicine group were significantly improved, compared with the normal control group, the CD4 of the model group was significantly decreased (P0.05), the CD_8~ significantly increased (P0.05). Compared with the model group, the level of CD_4~ T lymphocytes and the ratio of CD_4~ / CD_8~ were significantly increased and the number of CD_8~ T lymphocytes was decreased (P0.05). The expression of IL-6,PCT in serum of the model mice was higher than that of the normal group (P0.05), and the expression of IL-6,PCT in each treatment group was significantly lower than that in the model group (P0.01). Conclusion: Qingfei Peiyuan granule can effectively regulate the level of T lymphocyte subsets in peripheral blood of mice, reduce the expression of IL-6,PCT, and enhance the immune function of the body.
【作者单位】：河南中医药大学第一附属医院艾滋病临床研究中心河南省病毒性疾病中医药防治重点实验室;河南中医学院第二附属医院肺病科;
【基金】：河南省艾滋病中医药防治基础与临床研究创新型科技团队(No.C20140057) 国家中医药管理局临床科研基地科研专项(No.JDZX2015161) 河南省基础与前沿计划项目(No.132300413215)~~
【分类号】：R285.5;R-332

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