心肌微环境与5-氮杂胞苷诱导脂肪间充质干细胞心肌分化的差异（英文）
发布时间：2018-11-20 10:33
【摘要】：目的探讨体内心肌微环境及体外5-氮杂胞苷(5-aza)诱导脂肪间充质干细胞分化为心肌样细胞的作用差异。方法取小鼠腹股沟的皮下脂肪组织,分离培养脂肪间充质干细胞(ADMSCs),对其进行表面标记和成骨、成脂分化能力鉴定。选取生长状态良好的第3代ADMSCs,分为5-aza体外诱导组、体内心肌移植组,体外诱导3周以及体内移植1周后,采用免疫荧光技术检测特异性心肌肌钙蛋白T(cT nT)的表达。结果两种诱导方法ADMSCs均表达cT nT,5-aza诱导组3周表达率(33.33±3.79)%,移植组1周表达率(42.93±4.04)%,移植组1周较5-aza诱导组3周具有更高的分化效率(P0.05)。结论体外5-aza化学诱导和体内心肌微环境均可使ADMSCs分化为心肌样细胞,但心肌微环境的诱导分化效率明显高于5-aza的作用。
[Abstract]:Objective to investigate the effects of 5-azacytidine (5-aza) on differentiation of adipose mesenchymal stem cells into cardiomyocyte-like cells in vivo and in vitro. Methods the subcutaneous adipose tissue of mouse groin was isolated and cultured with (ADMSCs), for surface labeling and osteogenesis, and the ability of adipogenic differentiation was evaluated. The third generation of ADMSCs, with good growth state was divided into three groups: in vitro 5-aza induction group, in vivo myocardial transplantation group. The expression of cardiac troponin T (cT nT) was detected by immunofluorescence technique after 3 weeks of in vitro induction and 1 week after in vivo transplantation. Results the expression rate of cT nT,5-aza was (33.33 卤3.79)% in ADMSCs group and (42.93 卤4.04)% in transplantation group at 1 week. The differentiation efficiency of transplantation group was higher than that of 5-aza induction group at 1 week (P0.05). Conclusion both chemical induction of 5-aza in vitro and myocardial microenvironment in vivo can induce the differentiation of ADMSCs into cardiomyocyte-like cells, but the differentiation efficiency of myocardial microenvironment is significantly higher than that of 5-aza.
【作者单位】： 新乡医学院河南省医用组织再生重点实验室;新乡市中心医院心内科;
【基金】：National Natural Science Foundation of China(81400237) Key Project of He’nan Provincial Health Bureau(201403135) Xinxiang Medical University Graduate Student Research and Innovation Support Program(YJSCX201302Z)
【分类号】：R329.2
[Abstract]:Objective to investigate the effects of 5-azacytidine (5-aza) on differentiation of adipose mesenchymal stem cells into cardiomyocyte-like cells in vivo and in vitro. Methods the subcutaneous adipose tissue of mouse groin was isolated and cultured with (ADMSCs), for surface labeling and osteogenesis, and the ability of adipogenic differentiation was evaluated. The third generation of ADMSCs, with good growth state was divided into three groups: in vitro 5-aza induction group, in vivo myocardial transplantation group. The expression of cardiac troponin T (cT nT) was detected by immunofluorescence technique after 3 weeks of in vitro induction and 1 week after in vivo transplantation. Results the expression rate of cT nT,5-aza was (33.33 卤3.79)% in ADMSCs group and (42.93 卤4.04)% in transplantation group at 1 week. The differentiation efficiency of transplantation group was higher than that of 5-aza induction group at 1 week (P0.05). Conclusion both chemical induction of 5-aza in vitro and myocardial microenvironment in vivo can induce the differentiation of ADMSCs into cardiomyocyte-like cells, but the differentiation efficiency of myocardial microenvironment is significantly higher than that of 5-aza.
【作者单位】： 新乡医学院河南省医用组织再生重点实验室;新乡市中心医院心内科;
【基金】：National Natural Science Foundation of China(81400237) Key Project of He’nan Provincial Health Bureau(201403135) Xinxiang Medical University Graduate Student Research and Innovation Support Program(YJSCX201302Z)
【分类号】：R329.2
【相似文献】
相关期刊论文 前10条
1 仵敏娟,刘善荣,刘厚奇;间充质干细胞特性与应用前景[J];生命科学;2004年03期
2 史春梦;;间充质干细胞表述中需要注意的几个问题[J];第三军医大学学报;2009年14期
3 吴清法,王立生,吴祖泽;间充质干细胞的来源及临床应用[J];军事医学科学院院刊;2002年03期
4 郑春梅,孙昭,曹莹,孙琪云,马丽,马冠杰,姜学英,赵春华;胎儿肺脏来源间充质干细胞的鉴定与损伤修复的实验研究[J];中国生物工程杂志;2004年03期
5 余国建;间充质干细胞分化成骨的研究[J];国外医学.口腔医学分册;2004年S1期
6 宫立众,田小波,钟永明,夏顺中;间充质干细胞研究进展[J];重庆医学;2004年11期
7 王蓓,汪维伟;间充质干细胞的研究进展[J];国外医学.外科学分册;2005年06期
8 陈莉娜;王颖;张艳艳;关伟军;;间充质干细胞的免疫调节作用[J];中国组织工程研究与临床康复;2007年28期
9 阎新龙;刘兵;毛宁;;间充质干细胞迁移特性的研究进展[J];中国实验血液学杂志;2009年04期
10 黄q谀，
本文编号：2344676
本文链接：https://www.wllwen.com/xiyixuelunwen/2344676.html
最近更新
教材专著
