移植性卡波西肉瘤动物模型的建立

发布时间：2018-11-21 19:57

【摘要】：目的建立模拟卡波西肉瘤发展过程的移植性卡波西肉瘤的动物模型。方法将36只裸鼠随机分为A、B、C、D四个实验组,每组各9只。卡波西肉瘤细胞体外培养并计数,调节细胞悬液浓度为1×10~8/L、1×10~9/L、5×10~9/L、1×10~(10)/L,各取0.2 ml分别接种于四组实验组裸鼠背部皮下。观察各组裸鼠肿瘤长出时间,肿瘤体积及颜色变化,待瘤体长到36 d时处死裸鼠,取下肿瘤组织进行组织病理学检查及免疫组化CD31、CD34、AE1/AE3、S100、HHV-8。结果卡波西肉瘤细胞株皮下注射移植成瘤率为100%,肿瘤组织经免疫组化CD31、CD34染色阳性证实为血管来源肿瘤。4个实验组中裸鼠肿瘤均由斑点逐渐形成斑块、结节,各实验组小鼠瘤体体积随时间延长而逐渐增大,不同细胞浓度的实验组小鼠瘤体体积差异无统计学意义(P0.05)。第36天不同细胞浓度的实验组小鼠瘤体重量差异无统计学意义(P0.05)。结论成功建立了卡波西肉瘤细胞移植动物模型,且注射卡波西肉瘤细胞浓度越高,长出肿瘤的时间越短。
[Abstract]:Objective to establish an animal model of transplanted Kaposi's sarcoma which simulates the development of Kaposi's sarcoma. Methods Thirty-six nude mice were randomly divided into four experimental groups with 9 rats in each group. Kaposi sarcoma cells were cultured and counted in vitro. The cell suspension concentration was adjusted to 1 脳 10 ~ (8) / L ~ (-1) 脳 10 ~ (9) / L ~ (5 脳 10 ~ (9) L ~ (1 脳 10 ~ (10) / L). The tumor growth time, tumor volume and color changes were observed in each group. The nude mice were killed when the tumor was 36 days old, and the tumor tissue was removed for histopathological examination and immunohistochemical CD31,CD34,AE1/AE3,S100,HHV-8.. Results the rate of tumor formation by subcutaneous injection of Kaposi sarcoma cell line was 100. The tumor tissue was confirmed to be vascular origin tumor by immunohistochemical CD31,CD34 staining. The tumor volume of each experimental group increased gradually with the prolongation of time, and there was no significant difference in the tumor volume of the experimental group with different cell concentrations (P0.05). On the 36 th day, there was no significant difference in tumor weight in the experimental group with different cell concentrations (P0.05). Conclusion the animal model of Kaposi sarcoma transplantation was successfully established, and the higher the concentration of Kaposi sarcoma cells was, the shorter the time of tumor growth was.
【作者单位】：石河子大学医学院;新疆维吾尔自治区人民医院皮肤性病科;
【基金】：新疆维吾尔自治区自然科学基金(2014211A059)
【分类号】：R-332;R739.5

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