不同浓度弹力蛋白酶对建立兔腹主动脉瘤模型成瘤率的影响

发布时间：2018-11-24 12:46

【摘要】：目的通过腹主动脉腔内低压力灌注猪胰弹力蛋白酶建立兔腹主动脉瘤模型,探索不同浓度猪胰弹力蛋白酶对兔腹主动脉成瘤率的影响。方法新西兰雄兔50只,体质量(2.50±0.20)kg,随机分为5组:A组、B组、C组、D组、E组,每组各10只。A组经双腔导管注入生理盐水10μl,其余4组经双腔导管注入10μl浓度分别为1U/ml、10U/ml、100U/ml、200U/ml的猪胰弹力蛋白酶,灌注保持7min。术后第7天处死动物,分别于术前及术后第7天行腹主动脉彩超检查,观察灌注血管部位变化。结果 E组实验动物存活率较A组、B组、C组及D组明显下降(40%vs 90%、90%、90%及90%)。腹主动脉术后最大直径随给予灌注浓度增加而增大。与术前最大直径比较,A组及B组术后最大直径未见明显扩张;C组、D组及E组术后最大直径明显扩张;D组及E组较A组、B组及C组,术后第7天腹主动脉扩张率[(125.40±16.10)%及(248.20±5.60)%vs 0%、(24.90±2.50)%及(43.90±7.69)%,P0.05]及成瘤率[100.0%及100.0%vs 0%、0%及33.3%,P0.05]均明显升高。结论高浓度猪胰弹力蛋白酶(100U/ml)在低压力状态下较短时间灌注可成功建立兔腹主动脉瘤模型,且成瘤率高、死亡率低。
[Abstract]:Objective to establish a rabbit model of abdominal aortic aneurysm by perfusion of porcine pancreatic elastase into abdominal aorta under low pressure, and to explore the effect of different concentrations of porcine pancreatic elastase on the rate of abdominal aortic aneurysm formation in rabbits. Methods Fifty New Zealand male rabbits with a body weight of (2.50 卤0.20) kg, were randomly divided into five groups: group A, group B, group C, group D and group E. The other four groups were infused with porcine pancreatic elastase at the concentration of 1 U / ml 10 U / ml 100 U / ml / ml through double lumen catheter for 7 min. The animals were killed on the 7th day after operation. The abdominal aorta was examined by color Doppler ultrasonography before operation and on the 7th day after operation. Results the survival rate of experimental animals in group E was significantly lower than that in group A, group B, group C and group D (40%vs 90% and 90%). The maximum diameter of abdominal aorta increased with the increase of perfusion concentration. The maximum diameter of group A and group B was not significantly expanded compared with that before operation, and that of group C, group D and group E was significantly expanded after operation. The abdominal aortic dilatation rates in group D and E were (125.40 卤16.10)% and (248.20 卤5.60)% vs _ 0, (24.90 卤2.50)% and (43.90 卤7.69)% respectively on the 7th day after operation, compared with those in group A, B and C [(125.40 卤16.10)% and (248.20 卤5.60)%, respectively]. The rate of tumorigenesis [100.0%, 100.0%vs 0% and 33.3P] were significantly higher than that of control group (P 0.05). Conclusion High concentration porcine pancreatic elastase (100U/ml) can successfully establish rabbit abdominal aortic aneurysm model under low pressure for a short time, and the tumorigenesis rate is high and the mortality is low.
【作者单位】：首都医科大学附属北京安贞医院心内科;
【基金】：国家自然科学基金(81370437)
【分类号】：R-332;R543.1

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