河南省乙型肝炎病毒分型特征及小S蛋白主要亲水区变异特点分析
发布时间：2018-12-10 12:56
【摘要】：为了解河南省乙型肝炎(乙肝)HBV基因型分布及其主蛋白抗原主要亲水区(MHR)氨基酸(aa)位点变异情况。本研究采集河南省2012年HBV流行病学调查的部分HBsAg和HBeAg阳性的血清样本,提取HBV DNA并进行序列扩增,测序得到s基因序列,利用Mega6.0软件比较分析。共得到HBVS基因序列50条,基因型分布B型为16.0%(8/50)、C型为84.0%(42/50)。血清型分布中,adrq+为HBV主要流行血清型,流行率为84.0%。S基因MHR aa位点变异中,T126变异率最高,为14.0%。HBV MHR变异株总流行率为24.0%(12/50),其中B型的突变率为37.5%(3/8),C型的突变率为21.4%(9/42)。河南省乙肝基因型分布以C型为主,B型次之。血清型主要为adrq+为主,adw2次之。HBV MHR aa位点存在变异,应在今后的计划免疫和HBIG治疗中给予重视。
[Abstract]:To investigate the distribution of HBV genotypes and the variation of (MHR) amino acid (aa) sites in the main hydrophilic region of the main protein antigen in Henan Province. In this study, we collected some HBsAg and HBeAg positive serum samples from HBV epidemiological investigation in Henan province in 2012, extracted HBV DNA and sequenced it, sequenced the s gene sequence, and compared the results with Mega6.0 software. A total of 50 HBVS gene sequences were obtained. Genotype B was 16.0% (8- 50), C) and 84.0% (42 / 50). In the distribution of serotypes, adrq was the main prevalent serotype of HBV, the prevalence rate was 84.0.S gene MHR aa mutation, T126 mutation rate was the highest, and the total prevalence rate of 14.0%.HBV MHR variant was 24.0% (12 / 50). The mutation rate of B type was 37.5% (3 / 8), C) and the mutation rate was 21.4% (9 / 42). The distribution of hepatitis B genotype in Henan Province was mainly C type, followed by B type. The serotype is mainly adrq, and the. HBV MHR aa site of adw2 is the second. It should be paid attention to in the future planning immunization and HBIG therapy.
【作者单位】： 河南省疾病预防控制中心免疫规划所;中国疾病预防控制中心病毒病预防控制所;
【基金】：河南省科技厅基础与前沿技术研究计划项目(项目号:122300410054);题目:河南地区乙肝病毒分子流行特征暨单核苷酸多态性与肝病的关系研究 河南省卫生厅医学科技攻关项目(项目号:201203157);题目:河南地区乙肝病毒分子流行病学研究
【分类号】：R373.21
[Abstract]:To investigate the distribution of HBV genotypes and the variation of (MHR) amino acid (aa) sites in the main hydrophilic region of the main protein antigen in Henan Province. In this study, we collected some HBsAg and HBeAg positive serum samples from HBV epidemiological investigation in Henan province in 2012, extracted HBV DNA and sequenced it, sequenced the s gene sequence, and compared the results with Mega6.0 software. A total of 50 HBVS gene sequences were obtained. Genotype B was 16.0% (8- 50), C) and 84.0% (42 / 50). In the distribution of serotypes, adrq was the main prevalent serotype of HBV, the prevalence rate was 84.0.S gene MHR aa mutation, T126 mutation rate was the highest, and the total prevalence rate of 14.0%.HBV MHR variant was 24.0% (12 / 50). The mutation rate of B type was 37.5% (3 / 8), C) and the mutation rate was 21.4% (9 / 42). The distribution of hepatitis B genotype in Henan Province was mainly C type, followed by B type. The serotype is mainly adrq, and the. HBV MHR aa site of adw2 is the second. It should be paid attention to in the future planning immunization and HBIG therapy.
【作者单位】： 河南省疾病预防控制中心免疫规划所;中国疾病预防控制中心病毒病预防控制所;
【基金】：河南省科技厅基础与前沿技术研究计划项目(项目号:122300410054);题目:河南地区乙肝病毒分子流行特征暨单核苷酸多态性与肝病的关系研究 河南省卫生厅医学科技攻关项目(项目号:201203157);题目:河南地区乙肝病毒分子流行病学研究
【分类号】：R373.21
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