蒌芩止嗽煎对青霉素在慢性阻塞性肺疾病急性加重期痰热证大鼠模型肺组织转运能力的影响

发布时间：2018-12-11 04:42

【摘要】：目的:探讨蒌芩止嗽煎能否提高青霉素在慢性阻塞性肺疾病急性加重(AECOPD)痰热证大鼠模型肺组织中的转运能力。方法:80只Wistar雄性大鼠随机均分为模型组、氨溴索组、二陈汤组、蒌芩止嗽煎组。用熏吸香烟加气管内注射内毒素及鼻腔滴入金黄色葡萄菌,鼓风干燥箱中进行风热刺激的方法建立大鼠AECOPD痰热证模型。用高效液相色谱-质谱法检测各组大鼠不同时段生物标本中青霉素的浓度。结果:15min血清及支气管肺泡灌洗液中蒌芩止嗽煎组药物浓度较模型组、氨溴索组及二陈汤组显著增高(P0.05,P0.01);肺组织中各组比较无统计学意义,但中位数比较显示蒌芩止嗽煎组药物浓度最高。30min血清、肺组织各组比较无统计学意义,但在灌洗液中蒌芩止嗽煎组药物浓度均低于氨溴索组、二陈汤组和模型组(P0.05)。结论:清热化痰法指导下的蒌芩止嗽煎能提高青霉素在AECOPD痰热证大鼠模型肺组织中的药物浓度,即能增强其在肺组织中的转运能力,为临床选择中药作为COPD急性加重期抗生素治疗的辅助用药提供了实验依据。
[Abstract]:Objective: to explore whether the penicillin decoction can improve the transport ability of penicillin in the lung tissue of rats with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) with phlegm heat syndrome. Methods: 80 male Wistar rats were randomly divided into three groups: model group, ambroxol group, Erchen decoction group and trichosanthes Qinzisou decoction group. The rat model of phlegm heat syndrome (AECOPD) was established by cigarette smoking, endotoxin injection into trachea and nasal cavity drip of Grape aureus. The concentration of penicillin was determined by high performance liquid chromatography-mass spectrometry. Results: the drug concentration in serum of 15min and bronchoalveolar lavage fluid was significantly higher than that in model group, ambroxol group and Erchen decoction group (P0.05, P0.01). There was no significant difference in the lung tissue, but the median comparison showed that the drug concentration was the highest in the Luoqingzisou decoction group. There was no significant difference between the 30min serum and the lung tissue group. But in the lavage solution, the drug concentration in the decoction group was lower than that in the ambroxol group, Erchen decoction group and model group (P0.05). Conclusion: the penicillin can increase the concentration of penicillin in the lung tissue of rats with AECOPD phlegm heat syndrome, that is, it can enhance its transport ability in the lung tissue under the guidance of the method of clearing heat and removing phlegm. It provides experimental basis for clinical selection of traditional Chinese medicine as an adjuvant drug for the treatment of acute exacerbation of COPD.
【作者单位】：北京中医药大学东直门医院;中国中医科学院望京医院;
【基金】：国家自然科学基金青年基金资助项目(No.81202681)~~
【分类号】：R285.5;R-332

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