黄芩苷对溃疡性结肠炎模型大鼠NF-κB表达的影响

发布时间：2018-12-13 04:27

【摘要】：目的观察黄芩苷对溃疡性结肠炎模型大鼠核因子?κB(Nuclear factor?κB,NF-κB)表达及下游炎症细胞因子的影响。方法采用TNBS法制备大鼠溃疡性结肠炎模型,随机分为正常组、模型组、黄芩苷(Baicalin)高中低剂量组、阳性对照组(柳氮磺胺吡啶,SASP组)。给药10d后,留取结肠组织标本,观察结肠大体形态变化,采用ELISA法观察UC模型大鼠结肠组织中肿瘤坏死因子(TNF-α)、白介素-8(IL-8)和白介素-1(IL-1)的含量,采用Western blot法检测NF-κB的表达水平。结果与模型组相比,黄芩苷100mg/kg组能显著改善结肠大体形态,黄芩苷(25、50、100mg/kg)干预后能剂量依赖性的抑制各炎症因子的分泌,黄芩苷高剂量组能降低IL-1、IL-8和TNF-α表达水平,但与SASP组比较无明显差异。黄芩苷高剂量组能降低NF-κB的水平,促进IκBα水平(P0.05)。结论黄芩苷能够抑制NF-κB的活化,从而发挥其在溃疡性结肠炎中的抗炎效应。
[Abstract]:Objective to observe the effect of baicalin on the expression of nuclear factor-魏 B (Nuclear factor? 魏 B-魏 NF- 魏 B and inflammatory cytokines in rats with ulcerative colitis. Methods the ulcerative colitis model of rats was established by TNBS method. The rats were randomly divided into normal group, model group, baicalin (Baicalin) high and low dose group, and positive control group (SASP group). After 10 days of administration, the colonic tissue samples were collected to observe the changes of colonic gross morphology, and the tumor necrosis factor (TNF- 伪) in colonic tissue of UC model rats was observed by ELISA method. The levels of interleukin-8 (IL-8) and interleukin-1 (IL-1) were detected by Western blot assay. Results compared with the model group, baicalin 100mg/kg group could significantly improve the colonic gross morphology. Baicalin (2550 mg / kg) could inhibit the secretion of inflammatory factors in a dose-dependent manner, while baicalin high dose group could reduce IL-1,. The expression levels of IL-8 and TNF- 伪 were not significantly different from those of SASP group. Baicalin high dose group could decrease the level of NF- 魏 B and promote the level of I 魏 B 伪 (P0.05). Conclusion baicalin can inhibit the activation of NF- 魏 B and exert its anti-inflammatory effect in ulcerative colitis.
【作者单位】：南京中医药大学附属医院;南京中医药大学第一临床医学院;
【基金】：国家自然科学基金(81403344) 国家中医药行业科研专项资助(201407001) 江苏省临床医学科技专项项目资助(BL2014100) 国家中医临床研究基地(脾胃)资助江苏省高校优势学科项目资助
【分类号】：R285.5;R-332

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