结核分枝杆菌调节蛋白RelA结构和功能的生物信息学分析
发布时间：2018-12-14 06:01
【摘要】：目的应用生物信息学方法分析预测结核分枝杆菌调节蛋白RelA的结构及功能。方法从NCBI数据库获取结核分枝杆菌RelA蛋白的氨基酸序列,采用ProtParam工具预测蛋白质理化性质,采用Predict-Protein工具预测二级结构和疏水性,采用TMHMM软件包和TMpred程序对跨膜区预测,采用SignalP 4.1Server,用神经网络模型对信号肽预测,分析亚细胞定位、结构域和功能,运用SWISS-MODEL工具在线预测三维结构等。结果结核分枝杆菌RelA蛋白含有894个氨基酸,为不稳定性疏水性分泌蛋白,二级结构以螺旋结构和环状结构为主,无跨膜区,包含5个保守域,在胞浆中发挥生物学效应,调节(p)ppGpp合成。结论结核分枝杆菌RelA基因编码蛋白为不稳定性疏水性分泌蛋白,作为调节蛋白,主要参与细菌在生存压力下引起的严紧反应,调节(p)ppGpp的合成,因此可作为抗结核潜伏、持续感染治疗靶点。
[Abstract]:Objective to analyze and predict the structure and function of Mycobacterium tuberculosis regulatory protein RelA by bioinformatics. Methods the amino acid sequence of RelA protein of Mycobacterium tuberculosis was obtained from NCBI database. The physicochemical properties of the protein were predicted by ProtParam, the secondary structure and hydrophobicity were predicted by Predict-Protein, and the transmembrane region was predicted by TMHMM software package and TMpred program. SignalP 4.1 Server was used to predict the signal peptide with neural network model, to analyze subcellular localization, domain and function, and to use SWISS-MODEL tools to predict 3D structure online. Results the RelA protein of Mycobacterium tuberculosis contained 894 amino acids and was an unstable hydrophobic secretory protein. The secondary structure was mainly helical structure and annular structure with no transmembrane region and five conserved domains, which played a biological role in the cytoplasm. Regulate (p) ppGpp synthesis. Conclusion the RelA gene encoding protein of Mycobacterium tuberculosis is an unstable hydrophobic secretory protein, which is mainly involved in the severe response of bacteria under survival pressure and regulates the synthesis of (p) ppGpp, so it can be used as an antituberculous latent protein. Persistent infection is a therapeutic target.
【作者单位】： 潍坊医学院医学检验学系;潍坊医学院病原生物学教研室;
【基金】：国家自然科学基金项目(No.30972639) 山东省自然科学基金项目(No.ZR2016HM09)
【分类号】：R378.911
，
本文编号：2378056
[Abstract]:Objective to analyze and predict the structure and function of Mycobacterium tuberculosis regulatory protein RelA by bioinformatics. Methods the amino acid sequence of RelA protein of Mycobacterium tuberculosis was obtained from NCBI database. The physicochemical properties of the protein were predicted by ProtParam, the secondary structure and hydrophobicity were predicted by Predict-Protein, and the transmembrane region was predicted by TMHMM software package and TMpred program. SignalP 4.1 Server was used to predict the signal peptide with neural network model, to analyze subcellular localization, domain and function, and to use SWISS-MODEL tools to predict 3D structure online. Results the RelA protein of Mycobacterium tuberculosis contained 894 amino acids and was an unstable hydrophobic secretory protein. The secondary structure was mainly helical structure and annular structure with no transmembrane region and five conserved domains, which played a biological role in the cytoplasm. Regulate (p) ppGpp synthesis. Conclusion the RelA gene encoding protein of Mycobacterium tuberculosis is an unstable hydrophobic secretory protein, which is mainly involved in the severe response of bacteria under survival pressure and regulates the synthesis of (p) ppGpp, so it can be used as an antituberculous latent protein. Persistent infection is a therapeutic target.
【作者单位】： 潍坊医学院医学检验学系;潍坊医学院病原生物学教研室;
【基金】：国家自然科学基金项目(No.30972639) 山东省自然科学基金项目(No.ZR2016HM09)
【分类号】：R378.911
，
本文编号：2378056
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