骨通贴膏对甲醛疼痛模型大鼠的镇痛作用及机制研究
发布时间：2018-12-17 21:30
【摘要】：目的:研究骨通贴膏对甲醛疼痛模型大鼠的镇痛作用及机制。方法:60只SD大鼠随机均分为空白组,模型组,骨通贴膏低、中、高剂量组(0.594、1.188、2.376 g/贴,含生药0.48、0.96、1.92 g)与醋酸泼尼松组(ig给药,0.005 4 g/kg,外贴基质),采用甲醛法复制大鼠疼痛模型,造模后立即给药。采用电子压痛仪测定给药1、2、3、4、6 h后各组大鼠痛阈值;给药6 h后,于腹主动脉取血0.3 m L并处死大鼠,采用酶联免疫吸附试验(ELISA)测定大鼠血浆中β-内啡肽(β-EP)、前列腺素E2(PGE2)含量,分光光度法测定大鼠血清、炎症组织中一氧化氮(NO)含量,放射免疫法检测大鼠血清、炎症组织及脑组织中P物质含量。结果:与造模前比较,模型组大鼠各个时段痛阈值均降低(P0.05或P0.01);与空白组比较,模型组大鼠PGE2、NO、炎症组织和脑组织中P物质含量显著升高(P0.05或P0.01);与模型组比较,骨通贴膏各剂量组大鼠给药后对应时间点的痛阈值升高,PGE2、炎症组织与脑组织中P物质含量降低(P0.05或P0.01),骨通贴膏中、高剂量组β-EP含量升高、炎症组织中NO含量降低(P0.05或P0.01),骨通贴膏高剂量组血清中NO含量降低(P0.05)。结论:骨通贴膏具有一定的镇痛抗炎作用,其作用机制可能与降低PGE2、NO、P物质含量,升高β-EP含量有关。
[Abstract]:Objective: to study the analgesic effect and mechanism of Gutong plaster on formaldehyde pain model rats. Methods: sixty SD rats were randomly divided into blank group, model group, low, medium and high dose group (0.594L 1.1880.376g / plaster, containing 0.480.96g) and prednisone acetate group (ig, 0.005 g / kg). The rat model of pain was made by formaldehyde method and the drug was administered immediately after making the model. The pain threshold of rats in each group was determined by electronic tenderness apparatus after 6 hours of administration. After 6 hours of administration, blood samples were taken from abdominal aorta and the rats were killed. The plasma 尾-EP), prostaglandin E 2 (PGE2) was determined by enzyme linked immunosorbent assay (ELISA). The content of nitric oxide (NO) in serum and inflammatory tissue of rats was determined by spectrophotometry, and substance P in serum, inflammatory tissue and brain tissue was detected by radioimmunoassay. Results: compared with the model group, the pain threshold of the model group was significantly lower than that of the control group (P0.05 or P0.01). Compared with the blank group, the content of substance P in the inflammatory tissue and brain tissue of the model group was significantly higher than that in the control group (P0.05 or P0.01). Compared with the model group, the pain threshold at the corresponding time points was increased, the content of substance P in the inflammatory tissue and brain tissue of PGE2, was decreased (P0.05 or P0.01), and in the Gutong plaster, the pain threshold was increased at the corresponding time point after the administration of Gutong plaster (P0.05 or P0.01). The content of 尾-EP in high dose group increased, the content of NO in inflammatory tissue decreased (P0.05 or P0.01), and the content of NO in serum of high dose group of Gutong plaster decreased (P0.05). Conclusion: Gutong plaster has a certain analgesic and anti-inflammatory effect, and its mechanism may be related to decreasing the content of PGE2,NO,P and increasing the content of 尾-EP.
【作者单位】： 广州中医药大学科技产业园;广州中医药大学中药学院;桂林华润天和药业有限公司;广东新南方青蒿药业有限公司;
【基金】：广东省省级科技计划项目(No.2013B090800024,2014B040404066)
【分类号】：R285.5;R-332
本文编号：2384822
[Abstract]:Objective: to study the analgesic effect and mechanism of Gutong plaster on formaldehyde pain model rats. Methods: sixty SD rats were randomly divided into blank group, model group, low, medium and high dose group (0.594L 1.1880.376g / plaster, containing 0.480.96g) and prednisone acetate group (ig, 0.005 g / kg). The rat model of pain was made by formaldehyde method and the drug was administered immediately after making the model. The pain threshold of rats in each group was determined by electronic tenderness apparatus after 6 hours of administration. After 6 hours of administration, blood samples were taken from abdominal aorta and the rats were killed. The plasma 尾-EP), prostaglandin E 2 (PGE2) was determined by enzyme linked immunosorbent assay (ELISA). The content of nitric oxide (NO) in serum and inflammatory tissue of rats was determined by spectrophotometry, and substance P in serum, inflammatory tissue and brain tissue was detected by radioimmunoassay. Results: compared with the model group, the pain threshold of the model group was significantly lower than that of the control group (P0.05 or P0.01). Compared with the blank group, the content of substance P in the inflammatory tissue and brain tissue of the model group was significantly higher than that in the control group (P0.05 or P0.01). Compared with the model group, the pain threshold at the corresponding time points was increased, the content of substance P in the inflammatory tissue and brain tissue of PGE2, was decreased (P0.05 or P0.01), and in the Gutong plaster, the pain threshold was increased at the corresponding time point after the administration of Gutong plaster (P0.05 or P0.01). The content of 尾-EP in high dose group increased, the content of NO in inflammatory tissue decreased (P0.05 or P0.01), and the content of NO in serum of high dose group of Gutong plaster decreased (P0.05). Conclusion: Gutong plaster has a certain analgesic and anti-inflammatory effect, and its mechanism may be related to decreasing the content of PGE2,NO,P and increasing the content of 尾-EP.
【作者单位】： 广州中医药大学科技产业园;广州中医药大学中药学院;桂林华润天和药业有限公司;广东新南方青蒿药业有限公司;
【基金】：广东省省级科技计划项目(No.2013B090800024,2014B040404066)
【分类号】：R285.5;R-332
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1 夏菁;谢媛媛;张宗璇;张延惠;王义明;梁琼麟;罗国安;;骨通贴膏指纹图谱及化学成分研究[J];中成药;2014年03期
，本文编号：2384822
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