青海地区藏族男性饮酒行为与乙醇代谢酶基因ADH3和ALDH2多态型分布研究
发布时间：2018-12-18 08:26
【摘要】：目的探讨青海地区藏族男性饮酒行为模式及乙醛脱氢酶2(ALDH2)与乙醇脱氢酶3(ADH3)基因多态型分布。 方法(1)现场流行病学调查用于收集研究对象的饮酒行为。在青海省疾病预防控制中心及青海省藏医院的体检人群中,提前找出在当天体检的所有藏族男性,然后每隔一个体检号进行抽样。在青海大学医学院藏医系,以年级分层后采用整群抽样的方法抽取各班同学,被抽取班级的全部男同学进行调查。排除不愿意参与调查的人群。汉族抽取方法如上。所有被抽取的调查者均表示愿意参与该项目方可调查。三个采样点共抽取758人,剔除资料不完整者26份,实际分析资料640份,调查对象年龄为39.7±14.6。其中藏族430人,汉族302人,均为男性。(2)采用血液基因组DNA提取系统试剂盒提取DNA,聚合酶链式反应-限制性片段长度多态型(PCR-RFLP)方法对乙醇脱氢酶3(ADH3)和乙醛脱氢酶2(ALDH2)基因多态型进行检测。 结果(1)藏、汉族的整体饮酒率分别为66.47%和69.54%；藏、汉族人群中安全饮酒者分别占39.07%和41.39%,危险饮酒者分别占27.67%和28.16%；藏、汉族均以低度白酒为主分别占67.94%和64.28%；藏、汉族饮酒者中饮酒方式以众饮佐菜为主分别占79.44%和58.09%,其次为众饮不佐菜分别占12.89%和19.05%,差异有统计学意义(p0.01)；藏、汉族饮酒人群中认为饮酒是一种交友方式的分别57.49%和51.43%,其次是认为饮酒是一种享受的分别占27.87%和19.05%,差异有统计学意义(p0.01)；饮酒者脸红是发展成为危险饮酒者的保护因素,吸烟和身边人饮酒是危险饮酒者的危险因素。(2)藏、汉族ADH3*2等位基因频率分别占0.08和0.14,ALDH2*2等位基因的分别占0.22和0.19,ADH3*2和ALDH2*2等位基因在藏、汉族分布差异有统计学意义(p50.01)；藏族男性中组合基因型分布以野生纯合性ADH3和ALDH2占优势为50%,其次是正常性ADH3和缺陷型ALDH2基因组合型、缺陷型ADH3和正常性ALDH2和缺陷性ADH3和ALDH2基因组合型分别占35.11%、10.46%和4.41%。(3)藏族饮酒行为与ADH3、ALDH2基因相关,与ALDH2基因的更为密切。 结论藏族男性的饮酒率高,饮酒者年轻化,饮酒人群中仍存在不安全饮酒行为。藏族饮酒行为与ADH3、ALDH2基因具有相关性,与ALDH2基因的相关性更为密切。与汉族人群相比,藏族人群中ADH3*1和ALDH2*2占优势,表明藏族饮酒者酒后不良反应较汉族饮酒者多。藏族男性中以功能正常性ADH3和ALDH2基因组合型占优势,该类人群较其他基因组合型饮酒者更易发展成为酗酒者。同时有35.11%的人以正常型ADH3和缺陷型ALDH2基因组合型为主,该人群如果继续饮酒则对机体的损害将更为严重。健康工作者更加需要注意那些处于危险边缘的危险饮酒者和有害饮酒者,如果该危险饮酒者和有害饮酒者还没有发展到酒依赖的程度,能够早期及时的采取干预,能够有效的减少酒量和酒精所致的问题。
[Abstract]:Objective to investigate the pattern of drinking behavior and the distribution of acetaldehyde dehydrogenase 2 (ALDH2) and alcohol dehydrogenase 3 (ADH3) gene polymorphism in Tibetan men in Qinghai. Methods (1) the field epidemiological investigation was used to collect the drinking behavior of the subjects. In the physical examination population of the Center for Disease Control and Prevention of Qinghai Province and the Tibetan Hospital of Qinghai Province, all Tibetan men who were examined on the same day were identified in advance, and then sampled every other physical examination number. In the Department of Tibetan Medicine, College of Medicine, Qinghai University, every class was sampled by cluster sampling after grade stratification, and all the male students in the class were investigated. Exclude people who are unwilling to participate in the investigation. The Han nationality extraction method is as above. All respondents indicated that they were willing to participate in the project before the survey. A total of 758 people were sampled from three sampling sites, 26 samples were excluded from incomplete data, 640 actual data were analyzed, and the age of the subjects was 39.7 卤14.6. Of them, 430 were Tibetan and 302 were Han, all of them were male. (2) DNA, extraction kit was used to extract DNA,. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of ethanol dehydrogenase 3 (ADH3) and aldehyde dehydrogenase 2 (ALDH2) gene. Results (1) in Tibet, the overall drinking rate of Han nationality was 66.47% and 69.54 respectively, the safe drinking rate was 39.07% and 41.39%, the dangerous drinking rate was 27.67% and 28.1616%, respectively. 67.94% and 64.28% of the Han nationality were mainly low-alcohol liquor. Among the drinkers of the Han nationality, 79.44% and 58.09% of the drinkers were mainly mixed with vegetables, followed by 12.89% and 19.05, respectively. The difference was statistically significant (p0.01). Among the Han people, 57.49% and 51.43% considered drinking as a way of making friends, followed by 27.87% and 19.05% of them thought that drinking was a kind of enjoyment, the difference was statistically significant (p0.01). Blushing was the protective factor to develop into a dangerous drinker. Smoking and drinking were risk factors. (2) the frequency of ADH3*2 allele in Han nationality was 0.08 and 0.14, respectively. The percentage of ALDH2*2 alleles was 0.22 and 0.19, respectively, and the distribution of ADH3t2 and ALDH2*2 alleles was significantly different in Han nationality (p50.01). In Tibetan males, wild homozygous ADH3 and ALDH2 were dominant, followed by normal ADH3 and defective ALDH2 gene combinations, and defective ADH3 and normal ALDH2 and defective ADH3 and ALDH2 gene combinations accounted for 35.11%, respectively. 10.46% and 4.41% respectively. (3) Tibetan drinking behavior is related to ADH3,ALDH2 gene and is more closely related to ALDH2 gene. Conclusion the drinking rate of Tibetan males is high, the drinkers are younger, and unsafe drinking behavior still exists in the drinkers. Tibetan drinking behavior is correlated with ADH3,ALDH2 gene, and more closely with ALDH2 gene. Compared with the Han nationality, the ADH3*1 and ALDH2*2 were dominant in the Tibetan population, which indicated that the adverse effects of drinking were more in the Tibetan drinkers than in the Han drinkers. The functional normal ADH3 and ALDH2 gene combinations were dominant in Tibetan males, which were more likely to develop into alcoholics than those with other gene combinations. At the same time, 35.11% of the population were mainly composed of normal ADH3 and defective ALDH2 gene combination, and the damage to the body would be more serious if the population continued to drink. Health workers need to pay more attention to dangerous drinkers and harmful drinkers who are on the verge of danger and who are able to intervene early and in time if they have not developed a degree of alcohol dependence. Can effectively reduce alcohol consumption and alcohol caused by the problem.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R394
本文编号：2385628
[Abstract]:Objective to investigate the pattern of drinking behavior and the distribution of acetaldehyde dehydrogenase 2 (ALDH2) and alcohol dehydrogenase 3 (ADH3) gene polymorphism in Tibetan men in Qinghai. Methods (1) the field epidemiological investigation was used to collect the drinking behavior of the subjects. In the physical examination population of the Center for Disease Control and Prevention of Qinghai Province and the Tibetan Hospital of Qinghai Province, all Tibetan men who were examined on the same day were identified in advance, and then sampled every other physical examination number. In the Department of Tibetan Medicine, College of Medicine, Qinghai University, every class was sampled by cluster sampling after grade stratification, and all the male students in the class were investigated. Exclude people who are unwilling to participate in the investigation. The Han nationality extraction method is as above. All respondents indicated that they were willing to participate in the project before the survey. A total of 758 people were sampled from three sampling sites, 26 samples were excluded from incomplete data, 640 actual data were analyzed, and the age of the subjects was 39.7 卤14.6. Of them, 430 were Tibetan and 302 were Han, all of them were male. (2) DNA, extraction kit was used to extract DNA,. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of ethanol dehydrogenase 3 (ADH3) and aldehyde dehydrogenase 2 (ALDH2) gene. Results (1) in Tibet, the overall drinking rate of Han nationality was 66.47% and 69.54 respectively, the safe drinking rate was 39.07% and 41.39%, the dangerous drinking rate was 27.67% and 28.1616%, respectively. 67.94% and 64.28% of the Han nationality were mainly low-alcohol liquor. Among the drinkers of the Han nationality, 79.44% and 58.09% of the drinkers were mainly mixed with vegetables, followed by 12.89% and 19.05, respectively. The difference was statistically significant (p0.01). Among the Han people, 57.49% and 51.43% considered drinking as a way of making friends, followed by 27.87% and 19.05% of them thought that drinking was a kind of enjoyment, the difference was statistically significant (p0.01). Blushing was the protective factor to develop into a dangerous drinker. Smoking and drinking were risk factors. (2) the frequency of ADH3*2 allele in Han nationality was 0.08 and 0.14, respectively. The percentage of ALDH2*2 alleles was 0.22 and 0.19, respectively, and the distribution of ADH3t2 and ALDH2*2 alleles was significantly different in Han nationality (p50.01). In Tibetan males, wild homozygous ADH3 and ALDH2 were dominant, followed by normal ADH3 and defective ALDH2 gene combinations, and defective ADH3 and normal ALDH2 and defective ADH3 and ALDH2 gene combinations accounted for 35.11%, respectively. 10.46% and 4.41% respectively. (3) Tibetan drinking behavior is related to ADH3,ALDH2 gene and is more closely related to ALDH2 gene. Conclusion the drinking rate of Tibetan males is high, the drinkers are younger, and unsafe drinking behavior still exists in the drinkers. Tibetan drinking behavior is correlated with ADH3,ALDH2 gene, and more closely with ALDH2 gene. Compared with the Han nationality, the ADH3*1 and ALDH2*2 were dominant in the Tibetan population, which indicated that the adverse effects of drinking were more in the Tibetan drinkers than in the Han drinkers. The functional normal ADH3 and ALDH2 gene combinations were dominant in Tibetan males, which were more likely to develop into alcoholics than those with other gene combinations. At the same time, 35.11% of the population were mainly composed of normal ADH3 and defective ALDH2 gene combination, and the damage to the body would be more serious if the population continued to drink. Health workers need to pay more attention to dangerous drinkers and harmful drinkers who are on the verge of danger and who are able to intervene early and in time if they have not developed a degree of alcohol dependence. Can effectively reduce alcohol consumption and alcohol caused by the problem.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R394
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