三七治疗局灶性缺血性脑梗死模型小鼠的疗效及机制

发布时间：2018-12-22 09:25

【摘要】：目的观察三七对脑梗死模型小鼠的影响,探讨作用机制。方法将50只SD小鼠随机分成正常组、模型组、三七高剂量组、三七低剂量组和西药组。通过线栓法构建小鼠脑梗死模型。成模后,治疗组开始三七溶液灌胃给药12 w。N组和CT组给予等体积的生理盐水溶液。给药结束后观察空腹血糖、肝、肾脏器系数、血清中白细胞介素(IL)-18、肿瘤坏死因子(TNF)-α以及脑组织中超氧化酶歧化酶(SOD)、过氧化氢酶(CAT)等相关指标的含量,并通过实时定量聚合酶链反应(real-time PCR)检测Bcl-2、Bax mRNA水平。结果三七高剂量组及西药组空腹血糖比模型组有显著下降,而且三七治疗组及西药组均可升高Bcl-2的表达、下调Bax mRNA的表达(P0.05,P0.01)。结论三七可能通过升高Bcl-2的表达、下调Bax mRNA的表达,从而减轻局灶性缺血性小鼠脑梗死。
[Abstract]:Objective to observe the effect of Panax notoginseng on cerebral infarction mice and explore the mechanism. Methods 50 SD mice were randomly divided into normal group, model group, high dose group, low dose group and western medicine group. The model of cerebral infarction in mice was established by the method of thread embolization. After the model was established, the treatment group was given the same volume of normal saline solution by intragastric administration of Panax notoginseng solution 12 w.N and CT group. After administration, fasting blood glucose, liver and kidney organ coefficients, serum interleukin (IL) 18, tumor necrosis factor (TNF) 伪 and superoxidase dismutase (SOD), in brain tissue were observed. The content of catalase (CAT) and Bcl-2,Bax mRNA were detected by real-time quantitative polymerase chain reaction (real-time PCR). Results compared with the model group, the fasting blood glucose in the high dose group and the western medicine group of Panax notoginseng decreased significantly, and the expression of Bcl-2 was increased and the expression of Bax mRNA was down-regulated in the treatment group and the western medicine group (P0.05, P0.01). Conclusion Panax notoginseng may reduce focal ischemic cerebral infarction by increasing the expression of Bcl-2 and down-regulating the expression of Bax mRNA.
【作者单位】：河南省中医院脑病科;
【基金】：河南省中医药科学研究专项课题(2015zy2023)
【分类号】：R285.5;R-332

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