白豆蔻对阿霉素肾病模型大鼠肾脏组织TGF-β1、PAI-1表达的影响

发布时间：2018-12-24 07:54

【摘要】：目的:研究白豆蔻对阿霉素肾病大鼠的肾脏保护作用及其机制。方法:雄性Wistar大鼠60只,随机分为正常对照组、阿霉素肾病模型组、白豆蔻1.5 g/kg剂量组、白豆蔻3.0 g/kg剂量组、白豆蔻4.5 g/kg剂量组、尿毒清颗粒组。采用尾静脉一次性注射阿霉素(6.5mg/kg)建立阿霉素肾病模型,建立模型后第4d开始灌胃给药,连续给药3W。给药3W后代谢笼留取尿液,麻醉大鼠腹主动脉采血后处死动物并取肾脏组织,通过尿液分析仪检测各组大鼠尿液尿蛋白(PRO)、潜血(BLO)、胆红素(BIL)等指标;ELASE法检测血清Cys-C、尿液β2-MG的含量;免疫组化法检测肾脏组织TGF-β1、PAI-1的表达。结果:与模型组比较,各给药组均有不同程度的降低PRO、BLO、BIL含量,其中白豆蔻3.0 g/kg剂量组的作用优于其它给药组。ELASE法检测结果表明与模型组比较,白豆蔻三个剂量组均有降低Cys-C和β2-MG含量,其中白豆蔻3.0 g/kg剂量组和4.5 g/kg剂量组对Cys-C的作用优于低剂量组,白豆蔻3.0 g/kg剂量组对β2-MG的作用优于其它两个组;免疫组化实验结果表明与模型组比较肾组织TGF-β1、PAI-1的表达白豆蔻4.5 g/kg剂量组比较模型组明显减少,其中白豆蔻的各给药组对TGF-β1的表达没有明显区别。结论:白豆蔻能减轻阿霉素肾病大鼠的肾脏损伤,其作用机制可能与TGF-β1、PAI-1的表达有关。
[Abstract]:Aim: to study the renal protective effect and mechanism of cardamom on adriamycin nephropathy in rats. Methods: sixty male Wistar rats were randomly divided into normal control group, doxorubicin nephropathy model group, white cardamom 1.5 g/kg group, white nutmeg 3.0 g/kg dose group, white cardamom 4.5 g/kg dosage group and Niaoduqing granule group. The model of adriamycin nephropathy was established by single injection of adriamycin (6.5mg/kg) into tail vein. After 3 weeks of administration, the rats were sacrificed after taking blood from abdominal aorta of anesthetized rats. Urine protein (PRO), (BLO), bilirubin (BIL) were detected by urine analyzer. The content of 尾 2-MG in serum Cys-C, urine and the expression of TGF- 尾 1 and PAI-1 in renal tissue were detected by ELASE and immunohistochemistry. Results: compared with the model group, the content of PRO,BLO,BIL was decreased in different degree in each administration group, and the effect of 3. 0 g/kg group was better than that of the other groups. The results of ELASE assay showed that the content of PRO,BLO,BIL was higher than that of the model group. The contents of Cys-C and 尾 2-MG were decreased in the three dose groups, and the effect on Cys-C in the 3. 0 g/kg and 4. 5 g/kg groups was better than that in the low dose group. The effect of cardamom 3. 0 g/kg on 尾 2-MG was better than that of the other two groups. The results of immunohistochemistry showed that the expression of TGF- 尾 _ 1 and PAI-1 in renal tissue was significantly decreased in the model group compared with the model group. The expression of TGF- 尾 _ 1 in each administration group was not significantly different from that in the model group. Conclusion: Cardamom can attenuate the renal injury in rats with adriamycin nephropathy, and its mechanism may be related to the expression of TGF- 尾 1 and PAI-1.
【作者单位】：内蒙古民族大学附属医院;内蒙古民族大学蒙医药学院;
【分类号】：R285.5;R-332

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