流式细胞术分析PMA诱导THP-1分化为巨噬细胞的表型特征

发布时间：2018-12-24 10:59

【摘要】：目的用流式细胞术明确单独使用PMA诱导THP-1细胞分化而来的巨噬细胞的分化情况。方法首先用PMA刺激THP-1细胞分化为巨噬细胞,再分别使用LPS、IFN-γ和IL-6将细胞诱导为M1型巨噬细胞,用IL-4、IL-13和IL-6将细胞诱导为M2型巨噬细胞。显微镜下观察三种细胞的形态学改变,并用流式细胞术检测各细胞主要CD分子表达的差异。结果当THP-1分化为巨噬细胞后变为贴壁生长,细胞形态呈较规则的圆形或椭圆形;M1和M2细胞形态不规则,细胞突起较明显,细胞颗粒度较大。巨噬细胞表面与抗原提呈功能相关的CD分子的表达均较低,大部分呈阴性;而M1和M2细胞表面这些CD分子的表达均较高。结论单独使用PMA刺激THP-1细胞所分化而来的巨噬细胞抗原提呈能力较弱,基本处于静息状态。
[Abstract]:Objective to determine the differentiation of macrophages induced by PMA alone in THP-1 cells by flow cytometry. Methods PMA was used to stimulate the differentiation of THP-1 cells into macrophages, then LPS,IFN- 纬 and IL-6 were used to induce M 1 macrophages, and IL-4,IL-13 and IL-6 were used to induce M 2 macrophages. The morphological changes of the three kinds of cells were observed under microscope, and the differences of the expression of main CD molecules in the three cells were detected by flow cytometry. Results when THP-1 differentiated into macrophages, it became adherent to the wall, and the shape of the cells was regular round or ellipse, M1 and M2 cells were irregular in shape, the processes of cells were obvious, and the granularity of the cells was larger. The expression of CD molecules related to antigen presentation on macrophages was lower, most of them were negative, while those on M1 and M2 cells were higher. Conclusion the ability of antigen presentation of macrophages induced by PMA alone was weak and remained in a resting state.
【作者单位】：北京协和医学院比较医学中心中国医学科学院医学实验动物研究所卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室新发再发传染病动物模型研究北京市重点实验室;
【基金】：国家自然科学基金-青年科学基金项目(81301437) 科技部重大专项(2014ZX10001001-001-004,2014ZX10001001-002-006)
【分类号】：R392

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