香叶醇对神经痛模型大鼠的镇痛作用及机制研究

发布时间：2018-12-28 12:45

【摘要】：目的研究香叶醇对神经病理性疼痛的镇痛效用,并初步研究其发挥效用的可能机制。方法制作大鼠保留性神经痛(spared nerve injury,SNI)模型,在行为学水平检测给药前后大鼠机械痛阈的变化;体外急性分离SNI模型大鼠背根神经节细胞(dorsal root ganglion,DRG),膜片钳检测Na通道的活性变化;体外采用膜片钳技术分别检测稳定转染h Nav1.7通道和TRPA1通道的HEK293细胞,记录给药前后通道的活性变化。结果香叶醇能够快速发挥对SNI模型大鼠机械痛敏的镇痛效应,对急性分离的SNI模型大鼠DRG细胞Na通道表现明显抑制作用,对HEK293细胞株表达的h Nav1.7通道表现明显抑制作用,对HEK293细胞株表达的h TRPA1通道无抑制作用,高浓度下表现促进AITC激活h TRPA1通道作用。结论香叶醇可能通过抑制DRG细胞的Nav1.7通道起到对SNI大鼠机械痛敏的镇痛作用。
[Abstract]:Objective to study the analgesic effect of geraniol on neuropathic pain and its possible mechanism. Methods the rat model of (spared nerve injury,SNI was established and the mechanical pain threshold was measured before and after administration of the drug at the behavioral level. SNI model rat dorsal root ganglion cells (dorsal root ganglion,DRG) were isolated in vitro, and the changes of Na channel activity were detected by patch clamp. In vitro, patch clamp technique was used to detect the activity of h Nav1.7 channel and TRPA1 channel transfected HEK293 cells, respectively, and to record the changes of channel activity before and after administration of the drug. Results geraniol could exert the analgesic effect of mechanical pain sensitivity in SNI model rats, inhibit the Na channel of DRG cells in acute isolated SNI model rats, and inhibit the h Nav1.7 channels expressed in HEK293 cell lines. The h TRPA1 channel expressed in HEK293 cell line was not inhibited, but the h TRPA1 channel was activated by AITC at high concentration. Conclusion geraniol may have analgesic effect on mechanical pain sensitivity of SNI rats by inhibiting Nav1.7 channel of SNI cells.
【作者单位】：上海中药创新研究中心药理室;英属哥伦比亚大学脑研究中心;
【基金】：国家国际科技合作专项项目(No 2015DFA31630) 上海市科技支撑项目(No 13431900703)
【分类号】：R285.5;R-332

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