H3N2亚型禽流感病毒及猪流感病毒感染人肺腺癌细胞后增殖的差异性变化
发布时间：2018-12-29 13:47
【摘要】：人肺腺癌细胞A549被H3N2亚型禽流感病毒和猪流感病毒感染后,探讨不同毒株跨种属感染人呼吸道组织的可能性和趋势性。通过观察病毒感染A549细胞后的细胞病变(CPE)、血凝试验(HA)和50%组织细胞感染量(TCID50)来比较不同毒株感染细胞后的复制差异和毒力改变,发现同一亚型不同来源的流感病毒对A549细胞感染和复制能力有较大差异,哺乳动物来源的病毒更有感染人呼吸道细胞的趋势,SW/GX/NS2783/2010有潜在的感染人细胞的能力。感染过程中的细胞因子变化和病毒基因组组成特点是进一步研究的方向,应重点关注具有跨种属感染趋势的流感病毒及其特殊分子决定簇的组成和特点。
[Abstract]:Human lung adenocarcinoma cell line A549 was infected with H3N2 subtype avian influenza virus and swine influenza virus. (CPE), hemagglutination test (HA) and 50% histocyte infection (TCID50) were used to compare the difference of replication and virulence of A549 cells infected with virus. It was found that influenza viruses from different sources of the same subtype had significant differences in infection and replication ability of A549 cells. Mammalian viruses were more likely to infect human respiratory tract cells and SW/GX/NS2783/2010 had the potential ability to infect human cells. The changes of cytokines and the characteristics of viral genome composition during infection are the direction of further research. We should focus on the composition and characteristics of influenza viruses and their special molecular determinants which have the trend of cross-species infection.
【作者单位】： 广西医科大学微生物学教研室;
【基金】：广西研究生教育创新计划项目(YCBZ2014027) 广西医科大学青年科学基金项目(GXMUYSF201525)
【分类号】：R373
[Abstract]:Human lung adenocarcinoma cell line A549 was infected with H3N2 subtype avian influenza virus and swine influenza virus. (CPE), hemagglutination test (HA) and 50% histocyte infection (TCID50) were used to compare the difference of replication and virulence of A549 cells infected with virus. It was found that influenza viruses from different sources of the same subtype had significant differences in infection and replication ability of A549 cells. Mammalian viruses were more likely to infect human respiratory tract cells and SW/GX/NS2783/2010 had the potential ability to infect human cells. The changes of cytokines and the characteristics of viral genome composition during infection are the direction of further research. We should focus on the composition and characteristics of influenza viruses and their special molecular determinants which have the trend of cross-species infection.
【作者单位】： 广西医科大学微生物学教研室;
【基金】：广西研究生教育创新计划项目(YCBZ2014027) 广西医科大学青年科学基金项目(GXMUYSF201525)
【分类号】：R373
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