弹性蛋白抗体在豚鼠肺气肿模型中的表达
发布时间：2018-12-30 10:29
【摘要】：目的:通过弹性蛋白主动免疫及熏烟制造豚鼠肺气肿模型,观察各组模型中弹性蛋白抗体的产生及肺组织的破坏程度。探究弹性蛋白抗体自体免疫对肺气肿的影响。 方法:将30只雄性英国短毛豚鼠随机分为3组,分别为主动免疫组,熏烟组,对照组。主动免疫组于皮下多点注射弹性蛋白。熏烟组采用传统熏烟造模。对照组不做任何处理。造模满12周后检测各组豚鼠血清及肺泡灌洗液中的弹性蛋白抗体,并进行肺组织病理形态观察,同时测量各组模型的平均肺泡面积及平均肺泡间隔厚度。 结果:(1)主动免疫组及熏烟组肺组织结构均有不同程度的破坏,光镜下观察:主动免疫组肺泡壁有变薄及断裂,有肺大疱出现,可见少量炎性细胞浸润;熏烟组肺泡结构紊乱,肺泡相互融合成肺大疱,有大量炎性细胞浸润。(2)与对照组(720.86±139.86μm~2)相比,主动免疫组(977.70±221.69μm~2)及熏烟组(1344.24±309.01μm~2)平均肺泡面积明显增大(P0.05);且与对照组(3.74±1.50μm)相比,主动免疫组(3.26±1.49μm)及熏烟组(2.30±1.31μm)平均肺泡间隔厚度均变薄(P0.05)。而且,与熏烟组相比,主动免疫组平均肺泡面积较小(P0.05),平均肺泡间隔厚度较厚(P0.05)。(3)与对照组(1.29±0.46,0.25±0.14)相比,主动免疫组(2.14±0.29,1.73±0.33)及熏烟组(2.36±0.26,1.12±0.23)血清及肺泡灌洗液中弹性蛋白抗体均增多(P0.05)。主动免疫组血清及肺泡灌洗液中弹性蛋白抗体无明显差别(P0.05)。熏烟组血清中弹性蛋白抗体较肺泡灌洗液中明显增多(P0.05)。主动免疫组与熏烟组血清弹性蛋白抗体无明显差别(P0.05)。主动免疫组较熏烟组肺泡灌洗液中弹性蛋白抗体增多(P0.05)。 结论:弹性蛋白主动免疫可以促进豚鼠肺气肿形成。主动免疫组及熏烟组弹性蛋白抗体均明显增多,且两组肺组织均有不同程度破坏,表明弹性蛋白抗体自体免疫对肺气肿的发生有一定的促进作用。
[Abstract]:Aim: to establish a guinea pig model of emphysema by active immunization and fumigation of elastin, and to observe the production of elastin antibody and the degree of lung tissue damage in each group. To investigate the effect of self-immunization against elastin antibody on emphysema. Methods: thirty male British short-haired guinea pigs were randomly divided into three groups: active immunization group, fumigation group and control group. The active immunization group was injected with elastin at multiple subcutaneous points. The model of fumigation group was made with traditional fumigation. No treatment was given in the control group. After 12 weeks, the serum and alveolar lavage fluid of guinea pigs were detected for elastin antibodies, and lung histopathology was observed. The mean alveolar area and the mean alveolar septal thickness were measured at the same time. Results: (1) the lung tissue structure of active immunization group and fumigation group were destroyed to some extent. Under light microscope, the alveolar wall of active immunized group became thinner and ruptured, pulmonary bullae appeared, and a small amount of inflammatory cells infiltrated. In the fumigation group, the alveolar structure was disordered, the alveoli fused into bullae, and a large number of inflammatory cells infiltrated. (2) compared with the control group (720.86 卤139.86 渭 mm2), The mean alveolar area of active immunization group (977.70 卤221.69 渭 m) and fumigation group (1344.24 卤309.01 渭 m) was significantly increased (P0.05). Compared with the control group (3.74 卤1.50 渭 m), the mean thickness of alveolar septum in active immunization group (3.26 卤1.49 渭 m) and smoke group (2.30 卤1.31 渭 m) was thinner (P0.05). In addition, compared with the smoking group, the average alveolar area and the thickness of the alveolar septum in the active immunization group were smaller (P0.05), and the thickness of the average alveolar septum (P05 / 3) were higher than those in the control group (1.29 卤0.460.25 卤0.14). In the active immunized group (2.14 卤0.29) 1.73 卤0.33 and the fumigated group (2.36 卤0.261.12 卤0.23), the serum and alveolar lavage fluid antibodies were increased (P0.05). There was no significant difference in serum and alveolar lavage fluid (P 0.05). The level of serum elastin antibody in fumigation group was significantly higher than that in alveolar lavage fluid (P0.05). There was no significant difference in serum elastin antibody between active immunization group and fumigation group (P0.05). The level of elastin antibody in alveolar lavage fluid of active immunization group was higher than that of fumigation group (P0.05). Conclusion: active immunization with elastin can promote emphysema formation in guinea pigs. Both active immunization group and fumigation group increased the level of elastin antibody, and the lung tissues of both groups were damaged to some extent, which indicated that the autoimmunity of elastin antibody could promote the occurrence of emphysema.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R563.3;R-332
本文编号：2395451
[Abstract]:Aim: to establish a guinea pig model of emphysema by active immunization and fumigation of elastin, and to observe the production of elastin antibody and the degree of lung tissue damage in each group. To investigate the effect of self-immunization against elastin antibody on emphysema. Methods: thirty male British short-haired guinea pigs were randomly divided into three groups: active immunization group, fumigation group and control group. The active immunization group was injected with elastin at multiple subcutaneous points. The model of fumigation group was made with traditional fumigation. No treatment was given in the control group. After 12 weeks, the serum and alveolar lavage fluid of guinea pigs were detected for elastin antibodies, and lung histopathology was observed. The mean alveolar area and the mean alveolar septal thickness were measured at the same time. Results: (1) the lung tissue structure of active immunization group and fumigation group were destroyed to some extent. Under light microscope, the alveolar wall of active immunized group became thinner and ruptured, pulmonary bullae appeared, and a small amount of inflammatory cells infiltrated. In the fumigation group, the alveolar structure was disordered, the alveoli fused into bullae, and a large number of inflammatory cells infiltrated. (2) compared with the control group (720.86 卤139.86 渭 mm2), The mean alveolar area of active immunization group (977.70 卤221.69 渭 m) and fumigation group (1344.24 卤309.01 渭 m) was significantly increased (P0.05). Compared with the control group (3.74 卤1.50 渭 m), the mean thickness of alveolar septum in active immunization group (3.26 卤1.49 渭 m) and smoke group (2.30 卤1.31 渭 m) was thinner (P0.05). In addition, compared with the smoking group, the average alveolar area and the thickness of the alveolar septum in the active immunization group were smaller (P0.05), and the thickness of the average alveolar septum (P05 / 3) were higher than those in the control group (1.29 卤0.460.25 卤0.14). In the active immunized group (2.14 卤0.29) 1.73 卤0.33 and the fumigated group (2.36 卤0.261.12 卤0.23), the serum and alveolar lavage fluid antibodies were increased (P0.05). There was no significant difference in serum and alveolar lavage fluid (P 0.05). The level of serum elastin antibody in fumigation group was significantly higher than that in alveolar lavage fluid (P0.05). There was no significant difference in serum elastin antibody between active immunization group and fumigation group (P0.05). The level of elastin antibody in alveolar lavage fluid of active immunization group was higher than that of fumigation group (P0.05). Conclusion: active immunization with elastin can promote emphysema formation in guinea pigs. Both active immunization group and fumigation group increased the level of elastin antibody, and the lung tissues of both groups were damaged to some extent, which indicated that the autoimmunity of elastin antibody could promote the occurrence of emphysema.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R563.3;R-332
【参考文献】
相关期刊论文 前1条
1 许三林,吴人亮,陈春莲,郝春荣;上皮钙粘附素在吸烟小鼠呼吸道上皮损伤修复中表达的研究[J];中华结核和呼吸杂志;1999年07期
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