智三针对AD模型大鼠脑内ChAT及GLUT3表达的影响
发布时间：2019-01-06 17:40
【摘要】：目的:观察"智三针"对AD模型大鼠学习记忆能力、大脑皮质Ch AT及GLUT3的影响,探讨针刺治疗AD的机制。方法:采用腹腔注射D-gal联合双侧海马注射Aβ1-40建立AD模型,按随机数字表法分为正常组、模型组、非穴组、智三针组各15只。记录大鼠Y迷宫测试全天总反应时间(TRT)以评估其学习记忆水平,采用免疫组化染色法检测大鼠脑皮质内Ch AT、GLUT3的表达水平。结果:造模后,模型组、非穴组、智三针组大鼠的TRT较正常组均延长,4周治疗后与模型组、非穴组比较,智三针组大鼠TRT缩短;与正常组比较,模型组大鼠脑皮质Ch AT、GLUT3表达水平均显著降低,与模型组、非穴组比较,智三针组Ch AT、GLUT3表达水平均显著升高。结论:"智三针"能提高AD大鼠的学习记忆能力,其机制可能与升高大鼠脑内Ch AT、GLUT3的表达有关。
[Abstract]:Aim: to observe the effects of "Zhi San Acupuncture" on learning and memory ability, cerebral cortex Ch AT and GLUT3 in AD model rats, and to explore the mechanism of acupuncture on AD. Methods: the AD model was established by intraperitoneal injection of D-gal and bilateral hippocampal injection of A 尾 1-40. According to the method of random digital table, AD model was divided into normal group, model group, non-acupoint group and three-needle group. The total reaction time (TRT) was recorded to evaluate the learning and memory level of rats. The expression of Ch AT,GLUT3 in the cortex of rats was detected by immunohistochemical staining. Results: the TRT of the model group, the non-point group and the Zhi-San acupuncture group was longer than that of the normal group. After 4 weeks of treatment, compared with the model group and the non-point group, the TRT of the model group was shorter than that of the control group. Compared with the normal group, the expression of Ch AT,GLUT3 in the cerebral cortex of the model group was significantly decreased, and that of the model group and the non-point group was significantly higher than that of the model group and the non-acupoint group. Conclusion: "Zhi San Jing" can improve the ability of learning and memory in AD rats, and its mechanism may be related to the increase of Ch AT,GLUT3 expression in rat brain.
【作者单位】： 广州中医药大学针灸康复临床医学院;深圳市福田区中医院康复科;
【基金】：国家自然科学基金面上项目(81173350)-运用PET技术研究智三针对AD大鼠脑功能代谢的影响
【分类号】：R245;R-332
[Abstract]:Aim: to observe the effects of "Zhi San Acupuncture" on learning and memory ability, cerebral cortex Ch AT and GLUT3 in AD model rats, and to explore the mechanism of acupuncture on AD. Methods: the AD model was established by intraperitoneal injection of D-gal and bilateral hippocampal injection of A 尾 1-40. According to the method of random digital table, AD model was divided into normal group, model group, non-acupoint group and three-needle group. The total reaction time (TRT) was recorded to evaluate the learning and memory level of rats. The expression of Ch AT,GLUT3 in the cortex of rats was detected by immunohistochemical staining. Results: the TRT of the model group, the non-point group and the Zhi-San acupuncture group was longer than that of the normal group. After 4 weeks of treatment, compared with the model group and the non-point group, the TRT of the model group was shorter than that of the control group. Compared with the normal group, the expression of Ch AT,GLUT3 in the cerebral cortex of the model group was significantly decreased, and that of the model group and the non-point group was significantly higher than that of the model group and the non-acupoint group. Conclusion: "Zhi San Jing" can improve the ability of learning and memory in AD rats, and its mechanism may be related to the increase of Ch AT,GLUT3 expression in rat brain.
【作者单位】： 广州中医药大学针灸康复临床医学院;深圳市福田区中医院康复科;
【基金】：国家自然科学基金面上项目(81173350)-运用PET技术研究智三针对AD大鼠脑功能代谢的影响
【分类号】：R245;R-332
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