绞股蓝水提物对束缚水浸应激性胃溃疡模型大鼠的预防作用

发布时间：2019-01-07 10:39

【摘要】：目的:考察绞股蓝水提物对应激性胃溃疡模型大鼠的预防作用。方法:将60只大鼠随机分为正常组、模型组、阳性组(三九胃泰颗粒,3.69 g/kg)和绞股蓝水提物高、中、低剂量组(4.0、2.0、1.0 g/kg,以生药计),每组10只,ig给药,每天1次,连续1周。给药结束2 h后,除正常组外,其余各组大鼠均采用束缚水浸法复制应激性胃溃疡模型。造模3 h后,测定溃疡指数(UI)、溃疡抑制率,测定血清中超氧化物歧化酶(SOD)、丙二醛(MDA)以及胃黏膜组织中肿瘤坏死因子α(TNF-α)、前列腺素E2(PGE2)、一氧化氮(NO)、内皮素1(ET-1)水平。结果:与正常组比较,模型组大鼠血清中SOD水平降低、MDA水平升高(P0.05),胃黏膜组织中PGE2、NO水平降低,TNF-α、ET-1水平升高(P0.05);与模型组比较,各给药组大鼠UI明显降低、上述指标均明显改善,且具有一定的剂量依赖性,其中绞股蓝水提物高剂量组大鼠胃黏膜组织中PGE2、ET-1改善较阳性组更明显,差异均有统计学意义(P0.05)。结论:绞股蓝水提物可通过调控血清SOD-MDA平衡和胃黏膜组织NO-ET-1平衡、减轻胃黏膜损伤程度等方式发挥其预防胃溃疡的作用。
[Abstract]:Objective: to investigate the preventive effect of gynostemma pentaphyllum extract on stress gastric ulcer rats. Methods: sixty rats were randomly divided into normal group, model group, positive group (Sanjiuweitai granules, 3.69 g/kg) and water extract of gynostemma pentaphyllum with 10 rats in each group. Ig was given once a day for 1 week. After 2 hours of administration, the stress gastric ulcer model was established in all rats except the normal group with the method of bound water immersion. The ulcer inhibition rate of ulcer index (UI), superoxide dismutase (SOD),) malondialdehyde (MDA) (MDA) in serum, tumor necrosis factor 伪 (TNF- 伪) and prostaglandin E 2 (PGE2) in gastric mucosa were measured after 3 hours of modeling. Nitric oxide (NO), endothelin 1 (ET-1) level. Results: compared with the normal group, the serum levels of SOD, MDA, PGE2,NO, TNF- 伪 and ET-1 in the model group were decreased (P0.05), respectively (P0.05). Compared with the model group, the UI of rats in each administration group was significantly decreased, and the above indexes were improved obviously in a dose-dependent manner. The improvement of PGE2,ET-1 in gastric mucosa was more obvious in the high dose group of water extract of gynostemma pentaphyllum than that in the positive group. The difference was statistically significant (P0.05). Conclusion: the aqueous extract of gynostemma pentaphyllum can play a role in preventing gastric ulcer by regulating the balance of serum SOD-MDA and gastric mucosal tissue NO-ET-1 and reducing the degree of gastric mucosal injury.
【作者单位】：南阳医学高等专科学校药学院;
【分类号】：R285.5;R-332

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