T淋巴细胞亚群及维生素D水平与衰老的关系

发布时间：2019-01-19 18:23

【摘要】：目的探讨随年龄增长T淋巴细胞表型、血清25-羟维生素D[25-(OH)D]水平变化的规律。方法将216例健康体检对象按年龄分成3组,组1为非老年组(18~60岁)85例,组2为非高龄老年组(61~80岁)76例,组3为高龄组(80岁)55例;均采用四色免疫荧光标记流式细胞术分析外周血T淋巴细胞亚群、化学发光分析仪测定血25-(OH)D水平。结果 (1)各年龄组外周血T淋巴细胞亚群CD4~+、CD8~+都在正常范围内,组间无统计学差异(P0.05)。组1 CD3~+明显高于组2和组3(P0.05)。(2)组1CD95阳性明显高于组2和组3(P0.05)。(3)组2血清25-(OH)D水平明显低于组1和组3(P0.05)。(4)血清25-(OH)D水平与年龄呈负相关(r=-0.021,P0.05),与T细胞亚群CD95呈正相关(r=0.17,P0.05)。结论随着年龄增长T淋巴细胞亚群CD4~+、CD8~+变化不明显,CD3~+明显下降,CD95表达水平逐渐增高,而血清25-(OH)D逐渐降低,提示D95、25-(OH)D有可能成为评价免疫衰老潜在的生物学标志物。
[Abstract]:Objective to investigate the changes of T lymphocyte phenotype and serum 25 hydroxyvitamin D [25 (OH) D] level with age. Methods 216 healthy subjects were divided into three groups according to their age: group 1 (n = 85), group 2 (n = 76) (n = 61), group 3 (n = 55) (n = 80). The T lymphocyte subsets in peripheral blood were analyzed by four color immunofluorescence labeling flow cytometry and the blood 25-(OH) D levels were measured by chemiluminescence analyzer. Results (1) CD4~ and CD8~ of peripheral blood T lymphocyte subsets in all age groups were in normal range, and there was no statistical difference between groups (P0.05). The CD3~ of group 1 was significantly higher than that of group 2 and group 3 (P0.05). (2), and that of group 2 and group 3 (P0.05). (3) was significantly higher than that of group 2 and group 3 (P0.05). (3). The level of 25- (OH) D in group 2 was significantly lower than that in group 1 and group 3 (P0.05). (4) there was a negative correlation between serum 25- (OH) D level and age (r-0.021, r = 0.021). There was a positive correlation between T cell subsets and CD95 (r = 0.17, P 0.05). Conclusion the changes of CD4~ and CD8~ in T lymphocyte subsets were not obvious with age, but CD3~ decreased, CD95 expression increased gradually, and serum 25 (OH) D decreased gradually. These results suggest that D 95 (OH) D may be a potential biomarker for the evaluation of immune aging.
【作者单位】：江西省人民医院一部;
【基金】：江西省科技厅科技计划项目(20060140)
【分类号】：R339.38

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