补肾疗更浸膏对去势更年期模型大鼠神经内分泌功能失调的作用

发布时间：2019-02-16 00:25

【摘要】：目的研究补肾疗更浸膏对去势更年期模型大鼠神经内分泌功能失调的治疗作用及其作用机制。方法通过手术摘除雌性SD大鼠双侧卵巢复制更年期综合征模型。模型成功后,将其随机分为空白组,假手术组,模型组,戊酸雌二醇片组,坤泰胶囊组,补肾疗更浸膏低、中、高剂量组。末次给药24 h后,腹主动脉取血ELISA法测定血清雌二醇(E2)、促性腺激素释放激素(GnR H)、促黄体生成素(LH)、促卵泡激素(FSH)、睾酮(T)水平;取下丘脑测定多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)水平;取双侧子宫称湿重计算脏器指数;于10%甲醛溶液中固定,观察其病理学改变。结果补肾疗更浸膏可有效升高模型大鼠血清E2(P0.05,P0.01)水平,降低GnR H(P0.05,P0.01)、LH(P0.05,P0.01)、FSH(P0.05)、T(P0.05,P0.01)水平,有效降低下丘脑单胺类神经递质DA(P0.05,P0.01)、NE(P0.05,P0.01)、5-HT(P0.05,P0.01)、5-HIAA(P0.05,P0.01)水平,并可升高模型大鼠子宫系数(P0.05),增加子宫内膜厚度(P0.05)。结论补肾疗更浸膏可通过调节血液性激素与下丘脑单胺类神经递质水平,增强子宫功能,改善去势模型大鼠下丘脑-垂体-内分泌轴与植物神经功能的紊乱状态,从而有效稳定机体内环境,改善更年期神经内分泌系统功能,缓解更年期症状。
[Abstract]:Objective to study the therapeutic effect and mechanism of kidney tonifying and more extract on neuroendocrine dysfunction in castrated climacteric rats. Methods the model of menopausal syndrome was established by surgical excision of bilateral ovary of female SD rats. After the model was successful, it was randomly divided into blank group, sham-operation group, model group, estradiol valerate group, Kuntai capsule group, kidney tonifying treatment group with low, medium and high doses. Serum estradiol (E _ 2) and (FSH), testosterone (T) (FSH), a gonadotropin releasing hormone (GnR H),) -releasing hormone luteinizing hormone (LH), were measured by ELISA method in abdominal aorta 24 h after the last administration. The levels of dopamine (DA), norepinephrine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) were measured in hypothalamus, and the organ index was calculated by weighing both sides of uterus. It was fixed in 10% formaldehyde solution and its pathological changes were observed. Results the serum E2 (P0.05U P0.01) level and GnR H (P0.05U P0.01), LH (P0.05P0.01), FSH (P0.01), FSH (P0.05), T (P0.05P0.01) level were significantly increased in the model rats. The levels of hypothalamic monoamine neurotransmitter DA (P0.05P0.01), NE (P0.05P0.01), 5-HT (P0.05P0.01), 5-HIAA (P0.05P0.01) were significantly decreased. The uterine coefficient and endometrial thickness of the model rats were increased (P0.05) and the thickness of endometrium was increased (P0.05). Conclusion Kidney tonifying and more extract can improve the disorder of hypothalamus-pituitary-endocrine axis and autonomic nerve function by regulating the levels of blood sex hormones and hypothalamic monoamine neurotransmitters and enhancing the uterine function in ovariectomized rats. Thus effectively stabilizes the body's internal environment, improves the climacteric neuroendocrine system function, alleviates the climacteric symptom.
【作者单位】：湖南中医药大学中药现代化研究重点实验室;湖南中医药大学药学院;
【基金】：湖南省中药学重点学科(2014)
【分类号】：R285.5;R-332

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