高原低氧暴露小鼠脾脏T淋巴细胞数量减少且免疫活性下降

发布时间：2019-02-16 19:56

【摘要】：目的研究高原低氧条件下,小鼠脾脏T淋巴细胞亚群及其功能的改变。方法分别在400 m、2200 m和4200 m的不同海拔条件下饲养小鼠30 d后,ELISA检测脾细胞培养上清中白细胞介素4(IL-4)、γ干扰素(IFN-γ)的含量;MTT法检测小鼠脾脏T淋巴细胞的增殖能力;流式细胞术观察小鼠脾脏T淋巴细胞亚群的变化。结果高海拔低氧暴露30 d后,与对照组(海拔400 m)相比,4200 m和2200 m实验组小鼠脾脏指数明显增高,4200 m低氧组小鼠脾脏指数与2200 m低氧组相比也显著增加;小鼠脾细胞培养上清中IFN-γ的含量明显降低,而IL-4的水平未发生明显变化;脾脏T淋巴细胞增殖能力明显降低,同时,4200 m低氧组与2200 m低氧组相比,其T细胞增殖能力也显著下降;脾脏CD3~+T细胞、CD4~+T细胞、CD8~+T细胞数显著下降,其中,CD4~+T细胞数较CD8~+T细胞数下降明显,另外,4200 m低氧组与2200 m低氧组相比,CD3~+T细胞、CD4~+T细胞数也明显减少。结论海拔4200 m和2200 m高原低氧暴露30 d后,小鼠脾脏T淋巴细胞数目减少且增殖能力降低,IFN-γ水平降低。
[Abstract]:Objective to study the changes of spleen T lymphocyte subsets and their functions in mice under high altitude hypoxia. Methods ELISA was used to detect the levels of interleukin-4 (IL-4) and interferon 纬 (IFN- 纬) in the supernatant of spleen cell culture after the mice were fed at different altitudes of 200m and 4200 m for 30 days. The proliferative ability of spleen T lymphocytes and the changes of spleen T lymphocyte subsets were detected by MTT method and flow cytometry. Results after exposure to hypoxia at high altitude for 30 days, the spleen index of mice in the 4200 m and 2200 m groups was significantly higher than that in the control group (4200 m and 2200 m), and the spleen index in the 4200 m hypoxia group was significantly higher than that in the 2200 m hypoxia group. The content of IFN- 纬 in the supernatant of mouse spleen cell culture decreased significantly, but the level of IL-4 did not change significantly. The proliferation ability of splenic T lymphocytes was significantly decreased in the 4200 m hypoxia group compared with the 2200 m hypoxia group, and the T lymphocyte proliferation ability was also significantly decreased in the 4200 m hypoxia group compared with the 2200 m hypoxia group. The number of CD3~ T cells, CD4~ T cells and CD8~ T cells in spleen decreased significantly. The number of CD4~ T cells was significantly lower than that of CD8~ T cells. In addition, the number of CD3~ T cells and CD4~ T cells in 4200 m hypoxia group was significantly lower than that in 2200 m hypoxia group. Conclusion after exposed to hypoxia at altitude of 4200 m and 2200 m for 30 days, the number of T lymphocytes in spleen of mice decreased, the proliferation ability decreased, and the level of IFN- 纬 decreased.
【作者单位】：青海大学医学院;
【基金】：国家自然科学基金(81060249) 青海省应用基础研究计划项目(2015-ZJ-741,2014-ZJ-715,2013-Z-905) 青海大学2015年度中青年科研基金(2015-QYY-1)
【分类号】：R392

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