结核分枝杆菌Rv2629基因编码蛋白的生物信息学分析

发布时间：2019-02-20 21:14

【摘要】：目的应用生物信息学方法分析结核分枝杆菌Rv2629基因编码蛋白的结构和功能。方法从NCBI GenBank中获得结核分枝杆菌H37Rv利福平敏感菌株Rv2629的基因序列,利用在线分析软件预测和分析其编码蛋白的理化性质和亲疏水性,跨膜结构域及信号肽,糖基化位点及磷酸化位点,二级和三级结构及B、T淋巴细胞抗原表位。结果Rv2629蛋白由374个氨基酸组成,具有亲水性,主要存在于细胞壁及细胞膜。Rv2629含有信号肽及跨膜结构域,6个糖基化位点和4个磷酸化氨基酸位点,二级结构中α螺旋约占45.72%,β折叠约占17.11%。该蛋白含有3个潜在的B细胞抗原表位,其优势抗原表位主要集中在67-70,91-95,229-232位氨基酸,4个CTL细胞表位分别位于279、283、98、346位氨基酸,11个T h细胞表位分别位于26、127、256、25、34、116、204、215、350、363、367位氨基酸。结论Rv2629蛋白存在于细胞壁及细胞膜,是一分泌蛋白,在结核病的潜伏期可上调表达。该蛋白含有B、T细胞抗原表位,可作为结核利福平耐药株的研究靶点。
[Abstract]:Objective to analyze the structure and function of Rv2629 gene encoding protein of Mycobacterium tuberculosis by bioinformatics. Methods the gene sequence of rifampicin sensitive strain Rv2629 of Mycobacterium tuberculosis H37Rv was obtained from NCBI GenBank. The physical and chemical properties, hydrophobicity, transmembrane domain and signal peptide of the encoded protein were predicted and analyzed by on-line analysis software. Glycosylation sites and phosphorylation sites, secondary and tertiary structures, and B, T lymphocyte antigen surface sites. Results the Rv2629 protein was composed of 374 amino acids and was hydrophilic. Rv2629 contained signal peptide and transmembrane domain, 6 glycosylation sites and 4 phosphorylated amino acid sites. In the secondary structure, 伪 helix accounted for 45.72% and 尾 fold about 17.11%. The protein contains three potential B cell epitopes. The dominant epitopes are located at the amino acids at the 67-70G 91-95229-232 position, the four CTL cell epitopes are located at the 279U 283N 98346 amino acids, and the 11 T h cell epitopes are located at the amino acids at 269127U 256116244215350363367, respectively. Conclusion Rv2629 protein exists in the cell wall and cell membrane. It is a secretory protein and can up-regulate the expression in the latent period of tuberculosis. The protein contains B and T cell antigen, and can be used as a target of rifampicin-resistant tuberculosis.
【作者单位】：潍坊医学院医学检验学系;潍坊医学院病原生物学教研室;
【基金】：国家自然科学基金项目(No.30972639) 山东省自然科学基金项目(No.ZR2016HM09)
【分类号】：R378.911

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