布比卡因对大鼠炎性疼痛模型中脊髓胶质细胞的影响
发布时间：2019-02-22 08:07
【摘要】：目的：研究布比卡因对福尔马林炎性疼痛模型中脊髓胶质细胞及炎性因子的影响。 方法：成年雄性SD大鼠，体重200-220克，取大鼠72只，随机分为4组,假手术组：右足底掌部皮下注射0.1mL生理盐水；炎性痛组：右足底掌部皮下注射2.5%福尔马林试剂0.1mL，建立福尔马林炎性痛模型；布比卡因组：经蛛网膜下腔注射0.125%布比卡因40μL后同炎性痛组建立炎性疼痛模型；生理盐水组：经蛛网膜下腔注射40μL生理盐水后同炎性痛组建立炎性疼痛模型。于建立右足底掌部炎性疼痛模型的术前、术后1d、术后3d和术后5d分别观察机械缩足反射阈值（MWT）及累积疼痛评分，并于术后行为学实验结束后取L4-6脊髓进行Western blot方法测定脊髓小胶质细胞标记物（OX42）和星形胶质细胞标记物（GFAP）的蛋白表达水平及测定IL-1β、IL-6和TNF-α的分泌水平。 结果：术后1d，与炎性痛组和生理盐水组相比，，假手术组及布比卡因组大鼠MWT增高；累积疼痛评分下降（P＜0.05）；与假手术组相比，炎性痛组和生理盐水组大鼠脊髓OX42和GFAP的表达增加（P＜0.05）；与炎性痛组和生理盐水组相比，布比卡因组大鼠脊髓OX42和GFAP的表达降低（P＜0.05）。与假手术组相比，炎性痛组和生理盐水组大鼠脊髓IL-1β、IL-6和TNF-αmRNA表达上调；与炎性痛组和生理盐水组相比，布比卡因组大鼠脊髓IL-1β、TNF-αmRNA表达下调（P＜0.05），而IL-6表达水平在两组间的差异无统计学意义（P＞0.05）。 结论：在炎性疼痛模型中，布比卡因可抑制脊髓胶质细胞OX42和GFAP的表达及炎性因子IL-1β、TNF-α释放。
[Abstract]:Aim: to study the effects of bupivacaine on spinal glial cells and inflammatory factors in formalin inflammatory pain model. Methods: 72 adult male SD rats, weighing 200-220 grams, were randomly divided into 4 groups: sham operation group: right plantar palmar subcutaneous injection of 0.1mL saline; Inflammatory pain group: the right plantar palmar was subcutaneously injected with 2.5% formalin reagent 0.1 mL to establish the formalin inflammatory pain model. In bupivacaine group, the inflammatory pain model was established by injecting 0.125% bupivacaine 40 渭 L into subarachnoid space, and the inflammatory pain model was established by injecting 40 渭 L saline into subarachnoid space with inflammatory pain group. The mechanical foot reflex threshold (MWT) and cumulative pain score were observed before, 1 day, 3 days and 5 days after the establishment of the right plantar palmar inflammatory pain model. After the behavioral experiment, L4-6 spinal cord was taken to measure the protein expression of microglia marker (OX42) and astrocytic marker (GFAP) and IL-1 尾 by Western blot. The secretory levels of IL-6 and TNF- 伪. Results: on the 1st day after operation, compared with the inflammatory pain group and the saline group, the MWT of the sham operation group and the bupivacaine group were increased, the cumulative pain score was decreased (P < 0. 05). Compared with sham operation group, the expression of OX42 and GFAP in spinal cord of rats in inflammatory pain group and saline group was increased (P < 0. 05), and the expression of OX42 and GFAP in spinal cord of bupivacaine group was lower than that of inflammatory pain group and saline group (P < 0. 05). Compared with sham operation group, the expression of IL-1 尾, IL-6 and TNF- 伪 mRNA were up-regulated in inflammatory pain group and saline group. Compared with the inflammatory pain group and the saline group, the expression of IL-1 尾 and TNF- 伪 mRNA in the spinal cord of bupivacaine group was down-regulated (P < 0. 05), but the expression of IL-6 had no significant difference between the two groups (P > 0. 05). Conclusion: bupivacaine can inhibit the expression of OX42 and GFAP and the release of IL-1 尾 and TNF- 伪 in spinal glial cells in inflammatory pain model.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R614
本文编号：2428004
[Abstract]:Aim: to study the effects of bupivacaine on spinal glial cells and inflammatory factors in formalin inflammatory pain model. Methods: 72 adult male SD rats, weighing 200-220 grams, were randomly divided into 4 groups: sham operation group: right plantar palmar subcutaneous injection of 0.1mL saline; Inflammatory pain group: the right plantar palmar was subcutaneously injected with 2.5% formalin reagent 0.1 mL to establish the formalin inflammatory pain model. In bupivacaine group, the inflammatory pain model was established by injecting 0.125% bupivacaine 40 渭 L into subarachnoid space, and the inflammatory pain model was established by injecting 40 渭 L saline into subarachnoid space with inflammatory pain group. The mechanical foot reflex threshold (MWT) and cumulative pain score were observed before, 1 day, 3 days and 5 days after the establishment of the right plantar palmar inflammatory pain model. After the behavioral experiment, L4-6 spinal cord was taken to measure the protein expression of microglia marker (OX42) and astrocytic marker (GFAP) and IL-1 尾 by Western blot. The secretory levels of IL-6 and TNF- 伪. Results: on the 1st day after operation, compared with the inflammatory pain group and the saline group, the MWT of the sham operation group and the bupivacaine group were increased, the cumulative pain score was decreased (P < 0. 05). Compared with sham operation group, the expression of OX42 and GFAP in spinal cord of rats in inflammatory pain group and saline group was increased (P < 0. 05), and the expression of OX42 and GFAP in spinal cord of bupivacaine group was lower than that of inflammatory pain group and saline group (P < 0. 05). Compared with sham operation group, the expression of IL-1 尾, IL-6 and TNF- 伪 mRNA were up-regulated in inflammatory pain group and saline group. Compared with the inflammatory pain group and the saline group, the expression of IL-1 尾 and TNF- 伪 mRNA in the spinal cord of bupivacaine group was down-regulated (P < 0. 05), but the expression of IL-6 had no significant difference between the two groups (P > 0. 05). Conclusion: bupivacaine can inhibit the expression of OX42 and GFAP and the release of IL-1 尾 and TNF- 伪 in spinal glial cells in inflammatory pain model.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R614
【参考文献】
相关期刊论文 前3条
1 杨又春,毕好生;脊髓胶质细胞与疼痛调制机制[J];国外医学.麻醉学与复苏分册;2004年03期
2 孙怡,田玉科,王鹏,项红兵;异丙酚对大鼠脊髓星形胶质细胞体外激活的影响[J];中华麻醉学杂志;2004年10期
3 李清;刘菊英;李国华;朱涛;秦成名;周青山;;氯胺酮对谷氨酸诱导大鼠脊髓背角星形胶质细胞凋亡的影响[J];中华麻醉学杂志;2005年11期
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