结直肠癌中人类巨细胞病毒感染及其与Bcl-2、C-Jun表达的相关性研究

发布时间：2019-03-07 17:34

【摘要】：目的：检测结直肠癌患者肿瘤组织与瘤旁组织中人巨细胞病毒(human cytomegalovirus,HCMV)的感染情况,初步探讨人巨细胞病毒感染影响结直肠癌发生、发展的相关机制；检测Bcl-2、C-Jun蛋白在结直肠癌患者肿瘤组织及瘤旁组织中的表达水平,分析两者表达与人巨细胞病毒感染的相关性及意义,为今后研究结直肠癌的致病机制及治疗方法提供新的依据材料和方法：收集72例结直肠癌患者新鲜的手术切除组织,同时收集距离肿瘤组织手术切缘10cm以上自身瘤旁的正常肠粘膜组织为对照,应用RT-PCR技术检测组织中HCMV IE2表达水平；同时利用免疫组化SP法检测癌组织和癌旁组织中Bcl-2、C-Jun蛋白的表达水平。结果： 1.结直肠癌肿瘤组及瘤旁组HCMV IE2阳性率分别为37.5%(27/72)和5.6%(4/72),差异具有统计学意义(P0.05)。其阳性率与分化程度相关(P＜0.05),高／中分化中HCMV的IE2阳性率高于低分化 2.Bcl-2蛋白在结直肠癌肿瘤组织和瘤旁组织中阳性率分别为33.3%(24/72)和5.6%(4/72),差异具有统计学意义(P0.05),其阳性表达与Dukes分期程度(p0.05)相关,Dukes分期A+B阳性率高于Dukes分期C+D。 3.C-Jun蛋白在结直肠癌肿瘤组织和瘤旁组织中阳性率分别为40.3%(29/72)和6.9%((5/72),差异具有统计学意义(P0.05),其阳性表达与分化程度相关,高/中分化阳性率高于低分化。 4、27例HCMV IE2的结直肠癌肿瘤组织中C-Jun阳性者为18例,Bcl-2阳性者为17例；45例HCMV IE2阴性组织中C-Jun阴性者为40例,Bcl-2阴性者为38例。经统计学分析,HCMV感染与Bcl-2、C-Jun表达呈正相关。结论： 1、结直肠癌肿瘤组织中Bcl-2、C-Jun的表达明显高于瘤旁组织,提示Bcl-2、C-Jun基因的激活或者变异会增加结直肠癌发病的可能。 2、结直肠癌肿瘤组织中HCMV IE2阳性率明显高于瘤旁组织,经统计学分析与Bcl-2、C-Jun蛋白的表达呈正相关,提示结直肠癌中HCMV感染可能会通过影响Bcl-2、C-Jun的表达影响结直肠癌的发生发展。本实验结果为深入探讨结直肠癌与HCMV的相关关系供了新的理论和实验依据。
[Abstract]:Objective: to detect the infection of human cytomegalovirus (human cytomegalovirus,HCMV) in tumor tissues and paratumoral tissues of patients with colorectal cancer, and to explore the mechanism of human cytomegalovirus infection affecting the occurrence and development of colorectal cancer. To detect the expression level of Bcl-2,C-Jun protein in tumor tissues and paratumoral tissues of patients with colorectal cancer, and to analyze the correlation and significance between the expression of these two proteins and human cytomegalovirus infection. To provide new materials and methods for studying the pathogenesis and treatment of colorectal cancer in the future: to collect fresh resected tissues from 72 patients with colorectal cancer. At the same time, the normal intestinal mucosa adjacent to 10cm above the surgical margin of tumor tissue was collected as control. The expression level of HCMV IE2 in tumor tissue was detected by RT-PCR technique. Immunohistochemical SP method was used to detect the expression of Bcl-2,C-Jun protein in cancer tissues and paracancerous tissues. Results: 1. The positive rate of HCMV IE2 was 37.5% (27 / 72) in colorectal cancer group and 5.6% (4 / 72) in paratumoral group, the difference was statistically significant (P0.05). The positive rate was correlated with the degree of differentiation (P < 0.05). The positive rate of IE2 in high / middle differentiation of HCMV was higher than that of poorly differentiated 2.Bcl-2 protein in tumor tissues and adjacent tissues of colorectal cancer, the positive rates were 33.3% (24 / 72) and 5.6% (4 / 72), respectively. The difference was statistically significant (P0.05), and the positive expression was correlated with the degree of Dukes stage (p0.05). The positive rate of Dukes stage A B protein was higher than that of Dukes stage C D. 3.C-Jun protein was 40.3% (29 / 72) and 6.9% (5 / 72) in colorectal cancer tissues and paratumoral tissues, respectively. The difference was statistically significant (P0.05), the positive expression was correlated with the degree of differentiation, and the positive rate of high / middle differentiation was higher than that of low differentiation. In 27 cases of HCMV IE2, 18 cases were C-Jun positive, 17 cases were Bcl-2 positive, 40 cases were C-Jun negative and 38 cases were Bcl-2 negative in 45 HCMV IE2 negative tissues. Statistical analysis showed that there was a positive correlation between HCMV infection and Bcl-2,C-Jun expression. Conclusion: 1. The expression of Bcl-2,C-Jun in colorectal cancer is significantly higher than that in paratumoral tissue, suggesting that activation or mutation of Bcl-2,C-Jun gene may increase the possibility of colorectal cancer. 2. 2. The positive rate of HCMV IE2 in colorectal cancer tissues was significantly higher than that in paratumoral tissues, and there was a positive correlation between the expression of Bcl-2,C-Jun protein and the expression of HCMV protein, which suggested that HCMV infection in colorectal cancer might affect Bcl-2,. The expression of C-Jun affects the carcinogenesis and development of colorectal cancer. The results provide a new theoretical and experimental basis for the further study of the relationship between colorectal cancer and HCMV.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R735.3

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