纳米抗体异源表达的研究进展
发布时间：2019-03-12 15:30
【摘要】：羊驼体内存在天然缺失轻链的重链抗体(HcAb),其单域抗原结合片段也称为VHH或纳米抗体(nanobody,Nb),是目前已知的最小抗原结合片段。与传统抗体相比,纳米抗体具有分子量小(12~15kDa)、稳定性好和免疫原性低等特点,这些特点使得VHH在基础研究、诊断及治疗上具有极大的应用价值,目前已有多种纳米抗体进入了临床研究阶段。综述了近年来VHH在革兰氏阴性菌(大肠杆菌)、革兰氏阳性菌(芽孢杆菌和乳酸杆菌)、酵母、丝状真菌、昆虫细胞、哺乳动物细胞和植物细胞中异源表达的研究现状,包括表达系统、宿主、载体特点、载体构建方式及产量等;从分子水平、表达水平和理性设计三个层面探讨了纳米抗体产量提高的策略,以期为纳米抗体研究者提供借鉴和思路。
[Abstract]:There is a heavy chain antibody (HcAb), with natural deletion of light chain in alpaca, and its single domain antigen binding fragment is also called VHH or nano antibody (nanobody,Nb), which is the smallest antigen binding fragment known at present. Compared with traditional antibodies, nano-antibodies have the characteristics of small molecular weight (12~15kDa), good stability and low immunogenicity. These characteristics make VHH have great application value in basic research, diagnosis and treatment. At present, many kinds of nano-antibodies have entered the clinical research stage. This paper reviews the research status of heterologous expression of VHH in Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Bacillus and Lactobacillus), yeast, filamentous fungi, insect cells, mammalian cells and plant cells in recent years. Including expression system, host, vector characteristics, vector construction method and yield, etc. From the molecular level, expression level and rational design, the strategy to improve the production of nano-antibody was discussed in order to provide references and ideas for nano-antibody researchers.
【作者单位】： 天津大学化工学院系统生物工程教育部重点实验室;
【基金】：国家自然科学基金资助项目(31470967)
【分类号】：R392
本文编号：2438911
[Abstract]:There is a heavy chain antibody (HcAb), with natural deletion of light chain in alpaca, and its single domain antigen binding fragment is also called VHH or nano antibody (nanobody,Nb), which is the smallest antigen binding fragment known at present. Compared with traditional antibodies, nano-antibodies have the characteristics of small molecular weight (12~15kDa), good stability and low immunogenicity. These characteristics make VHH have great application value in basic research, diagnosis and treatment. At present, many kinds of nano-antibodies have entered the clinical research stage. This paper reviews the research status of heterologous expression of VHH in Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Bacillus and Lactobacillus), yeast, filamentous fungi, insect cells, mammalian cells and plant cells in recent years. Including expression system, host, vector characteristics, vector construction method and yield, etc. From the molecular level, expression level and rational design, the strategy to improve the production of nano-antibody was discussed in order to provide references and ideas for nano-antibody researchers.
【作者单位】： 天津大学化工学院系统生物工程教育部重点实验室;
【基金】：国家自然科学基金资助项目(31470967)
【分类号】：R392
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