缺氧环境下HSulf-1,HIF-1α在HaCaT细胞中的表达及HSulf-1过表达载体的构建
发布时间：2019-03-14 14:26
【摘要】：目的探讨HSulf-1,HIF-1α在缺氧时人角质形成细胞株HaCaT细胞中的表达并构建HSulf-1基因过表达载体。方法 Western blot法检测不同缺氧浓度下HaCaT细胞中HSulf-1,HIF-1α蛋白的表达;构建重组质粒p CDH-HSulf-1;Western blot法筛选HSulf-1过表达稳转HaCaT细胞株。结果 HSulf-1在缺氧条件下低表达,与HIF-1α表达呈负相关;成功筛选过表达HaCaT稳定细胞株。结论 HSulf-1低表达,与肿瘤细胞或肿瘤组织中表达一致,过表达可抑制肿瘤细胞增殖。推测过表达HSulf-1可能抑制角质形成细胞的增殖,构建HSulf-1基因过表达载体,便于进一步体内外研究与角质形成细胞增殖的影响。
[Abstract]:Objective to investigate the expression of HSulf-1,HIF-1 伪 in human keratinocyte line HaCaT under hypoxia and to construct HSulf-1 gene overexpression vector. Methods Western blot assay was used to detect the expression of HSulf-1,HIF-1 伪 protein in HaCaT cells under different hypoxia concentrations, and the recombinant plasmid p-CDH-HSulf-1;Western blot was constructed to screen the stably transfected HaCaT cell lines with HSulf-1 overexpression. Results the low expression of HSulf-1 was negatively correlated with the expression of HIF-1 伪 under hypoxia, and the stable cell lines expressing HaCaT were successfully screened. Conclusion the low expression of HSulf-1 is consistent with the expression in tumor cells or tissues, and over-expression can inhibit the proliferation of tumor cells. It is speculated that overexpression of HSulf-1 may inhibit the proliferation of keratinocytes and construct HSulf-1 gene overexpression vector, which is convenient for further study in vitro and in vivo and the effect of keratinocyte proliferation.
【作者单位】： 安徽医科大学上海皮肤病临床学院;上海市皮肤病医院;
【基金】：国家自然科学基金资助项目(81360236)
【分类号】：R364.4
，
本文编号：2440060
[Abstract]:Objective to investigate the expression of HSulf-1,HIF-1 伪 in human keratinocyte line HaCaT under hypoxia and to construct HSulf-1 gene overexpression vector. Methods Western blot assay was used to detect the expression of HSulf-1,HIF-1 伪 protein in HaCaT cells under different hypoxia concentrations, and the recombinant plasmid p-CDH-HSulf-1;Western blot was constructed to screen the stably transfected HaCaT cell lines with HSulf-1 overexpression. Results the low expression of HSulf-1 was negatively correlated with the expression of HIF-1 伪 under hypoxia, and the stable cell lines expressing HaCaT were successfully screened. Conclusion the low expression of HSulf-1 is consistent with the expression in tumor cells or tissues, and over-expression can inhibit the proliferation of tumor cells. It is speculated that overexpression of HSulf-1 may inhibit the proliferation of keratinocytes and construct HSulf-1 gene overexpression vector, which is convenient for further study in vitro and in vivo and the effect of keratinocyte proliferation.
【作者单位】： 安徽医科大学上海皮肤病临床学院;上海市皮肤病医院;
【基金】：国家自然科学基金资助项目(81360236)
【分类号】：R364.4
，
本文编号：2440060
本文链接：https://www.wllwen.com/xiyixuelunwen/2440060.html
最近更新
教材专著
