H7N9流感病毒在4种细胞中培养情况的研究

发布时间：2019-03-16 13:42

【摘要】：目的了解H7N9流感病毒在不同细胞中增殖的感染动力学差异。方法将H7N9流感病毒分别接种于鸡成纤维细胞(DF-1)、狗肾细胞(MDCK)、人支气管上皮细胞(BEAS-2B)、人肺腺癌细胞(A549),观察病毒在各细胞内增殖后细胞病变效应(CPE)、血凝实验滴度及50%组织培养细胞感染剂量。结果 H7N9流感病毒在A549细胞中培养36 h可达到血凝效价最高,同时可见明显CPE,在DF-1及MDCK细胞中培养72 h后血凝效价达到最高;在BEAS-2B细胞中增殖不明显,培养液对豚鼠血无明显凝集,且培养超过96 h尚无明显CPE。DF-1细胞、MDCK细胞、A549细胞对病毒的TCID50实验结果分别为10~(-4.34)、10~(-5.13)、10~(-3.84)。结论本实验使用的H7N9流感病毒不能有效感染BEAS-2B细胞并在其中增殖,但是可以在DF-1、MDCK及A549细胞中有效增殖。增殖能力以MDCK细胞最强,DF-1细胞略弱,A549细胞次之。A549细胞对病毒的吸附能力较强,可快速达到增殖的高峰。
[Abstract]:Objective to investigate the difference of infection kinetics of H7N9 influenza virus proliferation in different cells. Methods Influenza H7N9 virus was inoculated into chicken fibroblasts (DF-1), dog kidney cells (MDCK), human bronchial epithelial cells (BEAS-2B) and human lung adenocarcinoma cells (A549), respectively. To observe the cytopathic effect of (CPE), hemagglutination test titers and 50% tissue culture cell infection dose after virus proliferation in each cell. Results the hemagglutination titer of H7N9 influenza virus was the highest in A549 cells for 36 hours, and that of CPE, was the highest after 72 hours of culture in DF-1 and MDCK cells. There was no obvious proliferation in BEAS-2B cells, no obvious agglutination to guinea pig blood in the culture medium, and no obvious CPE.DF-1 cells in culture for more than 96 h. The TCID50 test results of MDCK cells and A549 cells to virus were 10 ~ (- 4.34), respectively. 10 ~ (- 5.13), 10 ~ (- 3.84). Conclusion the H7N9 influenza virus can not infect and proliferate BEAS-2B cells effectively, but it can proliferate effectively in DF-1,MDCK and A549 cells. The proliferation ability of MDCK cells was the strongest, that of DF-1 cells was slightly weaker, and that of A549 cells was the second. A549 cells had a strong ability to adsorb virus, and could reach the peak of proliferation rapidly.
【作者单位】：深圳市福田区疾病预防控制中心;深圳市疾病预防控制中心;
【基金】：2014年深圳市福田区卫生公益性科研项目(FTWS201-4056)
【分类号】：R373.13

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