小鼠感染细小病毒的临床特征分析

发布时间：2019-03-17 13:37

【摘要】：目的分析小鼠细小病毒(MVM)在人工感染小鼠体内的分布规律、排毒和抗体变化,以及自然条件下小鼠感染MVM的情况。方法对33只BALB/c小鼠腹腔接种0.2 m L MVM悬浮液,每天观察动物的外观、行为、饮食和精神状态,在接种第0~60天共12个时间点各对2~3只动物进行安乐死,并采取组织、粪便和血清样本进行检测。荧光定量PCR(QPCR)方法检测组织和粪便的病毒核酸,ELISA方法检测血清抗体。同时,采取100只SPF小鼠、76只开放饲养小鼠检测MVM核酸,采取1463只SPF小鼠、82只开放饲养小鼠检测MVM抗体。结果实验小鼠接种MVM后外观、行为、饮食和精神状态未见异常,剖检无明显病变。各组织在接种后都能检出病毒核酸,并在接种后4~7 d达到峰值,且到60 d仍可检出病毒核酸。各组织间比较,病毒峰值最高的组织是肝,其次是肾、脾、胃、心脏、肺、盲肠和脑。粪便排毒在接种后11 d可达到峰值,之后迅速下降,但到60 d还可检到。血清抗体在病毒接种后7 d开始产生,之后抗体效价逐渐升高,21 d就可以达到32倍左右,之后至60 d一直处于64倍左右。临床样本中,核酸检测SPF小鼠未检出,开放饲养小鼠的检测阳性率为14.5%,经过测序比较确认为MVM病毒感染;抗体检测SPF小鼠有较低的检出率(0.3%),开放饲养小鼠检出率为68.3%。结论 MVM感染小鼠后一般呈隐性感染状态,可长期排毒,主要组织脏器、粪便、血清都能用于MVM的检测,实验小鼠感染MVM可以通过病毒核酸和血清抗体检测方法进行病原检测。
[Abstract]:Aim to analyze the distribution of mouse parvovirus (MVM) in artificially infected mice, the changes of detoxification and antibody, and the infection of MVM in mice under natural conditions. Methods 33 BALB/c mice were inoculated intraperitoneally with 0.2 m L MVM suspension. The appearance, behavior, diet and mental state of the animals were observed every day. 3 animals were euthanized at 12 time points from 0 to 60 days after inoculation, and the tissues were taken. Feces and serum samples were tested. Fluorescent quantitative PCR (QPCR) method was used to detect viral nucleic acid in tissues and feces, and ELISA method was used to detect serum antibodies. At the same time, 100 SPF mice, 76 open feeding mice, 1463 SPF mice and 82 open feeding mice were used to detect MVM nucleic acid. MVM antibody was detected in 82 open feeding mice. Results the appearance, behavior, diet and mental state of the mice inoculated with MVM were not abnormal, and no obvious pathological changes were observed. Viral nucleic acid was detected in all tissues after inoculation, and reached its peak at 4 days after inoculation and reached its peak value at 7 days after inoculation, and virus nucleic acid could still be detected at 60 days after inoculation. The highest viral peak was found in liver, followed by kidney, spleen, stomach, heart, lung, cecum and brain. Fecal efflux reached its peak at 11 d after inoculation, then decreased rapidly, but it could be detected by 60 d. Serum antibodies began to be produced 7 days after inoculation, and then the titer of antibodies increased gradually, reaching about 32 times on 21 days and 64 times on 60 days after inoculation. In clinical samples, no SPF mice were detected by nucleic acid assay. The positive rate of open feeding mice was 14.5%. The positive rate was confirmed to be MVM virus infection by sequencing. The detection rate of antibody in SPF mice (0.3%) was lower than that in open feeding mice (68.3%). Conclusion MVM-infected mice are usually in the state of recessive infection, which can be used for long-term detoxification. The main tissues, organs, feces and serum can be used for the detection of MVM. MVM infection in experimental mice can be detected by means of viral nucleic acid and serum antibody detection.
【作者单位】：广东省实验动物监测所;广东省实验动物重点实验室;
【基金】：广东省科技计划项目(2014B070706006,2013B060400028,2014A070705003)
【分类号】：R-33

【相似文献】