补肾活血通淋方对良性前列腺增生症大鼠模型Bcl-2、Bax影响的研究

发布时间：2019-03-23 16:48

【摘要】：目的：本项目旨在通过前列腺指数、凋亡抑制基因Bcl-2、凋亡促进基因Bax来研究补肾活血通淋方治疗良性前列腺增生症的作用机制，评价补肾活血通淋方对良性前列腺增生症大鼠模型Bcl-2和Bax的影响，为中医药诊治良性前列腺增生症提供较为科学的理论和实验依据。方法：雄性Wistar大鼠60只，编号称重，随机分为6组，每组10只，即假手术组、模型组、保列治组（阳性对照组）、补肾活血通淋方低剂量组、补肾活血通淋方中剂量组、补肾活血通淋方高剂量组。大鼠适应性喂养1周后开始造模。各组均腹腔注射10％的水合氯醛（3.5mg/kg）麻醉，无菌操作下进行手术，经腹腔摘除双侧睾丸，假手术组仅麻醉后切开腹腔，游离并不摘除双侧睾丸。术后恢复7天，假手术组不注射丙酸睾酮，给予10ml/kg生理盐水，余5组按造模方法（每只大鼠每天皮下注射丙酸睾酮5mg/kg）造模，造模开始后，模型组给予10ml/kg生理盐水，保列治组给予0.8mg/kg，（溶于10ml生理盐水，相当于临床用药量的10倍），补肾活血通淋方低剂量组给予5ml/kg（相当于临床用药量的5倍）、补肾活血通淋方中剂量组给予10ml/kg（相当于临床用药量的10倍），补肾活血通淋方高剂量组给予15ml/kg（相当于临床用药量的15倍）。以上均采用灌胃给药，1天1次，每周称体重1次，以调节给药用量，连续30天。末次给药24h后，，断头处死动物，取前列腺组织，分组标记，大鼠前列腺用电光分析天平称重，获取前列腺湿重，同时制作光镜和免疫组化标本，对各组标本进行分析并观察光镜下前列腺细胞形态变化。结果：3个中药治疗组大鼠前列腺湿重、前列腺指数及Bcl-2表达水平均明显低于模型组，且Bax的表达水平均明显高于模型组，其中尤其以补肾活血通淋方高剂量组最为明显。补肾活血通淋方高、中剂量组与保列治组比较有统计学意义；补肾活血通淋方低剂量组与保列治组比较时，Bcl-2表达水平有统计学意义，Bax表达水平无统计学意义。模型组大鼠前列腺组织可见明显的病理性改变，补肾活血通淋方低剂量组与保列治组大鼠前列腺组织的病理性改变较模型组减轻，补肾活血通淋方中剂量组大鼠前列腺组织的病理性改变较模型组减轻，补肾活血通淋方高剂量组大鼠前列腺组织的病理性改变较模型组显著减轻，各组大鼠前列腺组织病理学改变均有统计学意义。
[Abstract]:Objective: to study the mechanism of Bushen Huoxue Tongling recipe (BXT) in the treatment of benign prostatic hyperplasia (BPH) by means of prostate index and apoptosis suppressor gene Bcl-2, apoptosis promoting gene Bax. To evaluate the effect of Bushen Huoxue Tongling recipe on Bcl-2 and Bax in rat model of benign prostatic hyperplasia, and to provide scientific theoretical and experimental basis for the diagnosis and treatment of benign prostatic hyperplasia by traditional Chinese medicine. Methods: sixty male Wistar rats were randomly divided into 6 groups with 10 rats in each group, namely sham operation group, model group, Baoliao group (positive control group), low dose group of Bushen Huoxue Tongling recipe and middle dose group of Bushen Huoxue Tongling recipe. High dose group of Bushen Huoxue Tongling prescription. The rats were fed adaptively for 1 week and the model was made. In each group, 10% chloral hydrate (3.5mg/kg) was injected intraperitoneally and operated under aseptic operation. Bilateral testes were removed through abdominal cavity. In sham operation group, abdominal cavity was cut only after anesthesia, and bilateral testes were not removed free. Seven days after the operation, no testosterone propionate was injected into the sham operation group and 10ml/kg saline was given. The remaining 5 groups were modeled by subcutaneous injection of testosterone propionate 5mg/kg per day in the remaining 5 groups. After the establishment of the model, the rats in the sham operation group were given normal saline. The model group was given 10ml/kg saline, the Paulie group was given 0.8mg / kg, (dissolved in 10ml saline, equivalent to 10 times the clinical dosage), and the low dose group of Bushen Huoxue Tongling recipe was given 5ml/kg (equivalent to 5 times the clinical dosage). The middle dose group of Bushen Huoxue Tongling recipe was given 10ml/kg (equivalent to 10 times of clinical dosage) and the high dose group of Bushen Huoxue Tongling recipe was given 15ml/kg (equivalent to 15 times of clinical dosage). All of them were administered intragastrically once a day and weighed once a week to regulate the dosage of the drug for 30 consecutive days. 24 hours after the last administration, the animals were killed by decapitation, the prostate tissue was taken and labeled in groups. The rat prostate was weighed by electro-light analysis balance, and the wet weight of the prostate was obtained. At the same time, the specimens of light microscope and immunohistochemistry were made. The morphological changes of prostate cells were observed under light microscope. Results: the wet weight of prostate, prostate index and expression of Bcl-2 in three groups were significantly lower than those in model group, and the expression level of Bax was significantly higher than that in model group, especially in the high dose group of Bushen Huoxue Tongling Formula. The expression level of Bcl-2 was statistically significant in the low dose group of Bushen Huoxuetongling recipe and the group of Baolizhi, but there was no statistical significance in the expression level of Bax in the low dose group of Bushen Huoxue Tongling recipe and in the group of Baolingzhi (P < 0.05). Obvious pathological changes were observed in the prostate tissue of the model group. The pathological changes of the prostate tissue in the low dose group of Bushen Huoxue Tongling recipe and Baolizhi group were less than those in the model group. The pathological changes of prostate tissue in the middle dose group of Bushen Huoxue Tongling recipe were less than those in the model group, and the pathological changes of the prostate tissue in the high dose group of Bushen Huoxue Tongling recipe were significantly less than those in the model group. The histopathological changes of prostate in each group were statistically significant.
【学位授予单位】：河南中医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R277.5

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