愈癫汤对地卓西平所致精神分裂症模型大鼠氧化应激防御系统的影响

发布时间：2019-03-25 18:59

【摘要】：目的:基于多巴胺(DA)神经元功能亢进理论,观察愈癫汤对精神分裂症模型大鼠额前脑组织抗氧化应激防御系统相关指标的影响。方法:将48只雄性成年SD大鼠随机分为空白组、模型组、对照组及治疗组,每组12只。其中模型组、对照组及治疗组应用地卓西平腹腔注射造模,空白组应用0.9%氯化钠溶液行相同方法腹腔注射,连续14d;于第15天进行干预性给药,对照组给予0.036mg/m L利培酮悬浊液灌胃,治疗组给予0.92g/m L愈癫汤水煎剂灌胃,空白组、模型组给予相应体积蒸馏水灌胃,连续14d;检测各组大鼠额前脑组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)、过氧化氢酶(CAT)活性及DA的表达情况。结果:治疗组大鼠额前组织内SOD、GSH及CAT活性明显高于模型组(P0.05),而DA的表达明显低于模型组(P0.05)。结论:愈癫汤可通过促进脑组织中SOD、GSH及CAT活性,将超氧化物转化成无毒的水,从而抑制自由基的形成,阻断氧化应激反应,从而逆转DA神经元功能亢进引起的氧化应激反应。
[Abstract]:Aim: based on the theory of dopaminergic (DA) neuron hyperfunction, the effects of Yuwei decoction on the indexes of antioxidant stress defense system in frontal forebrain of schizophrenic rats were observed. Methods: 48 adult male SD rats were randomly divided into blank group, model group, control group and treatment group with 12 rats in each group. The model group, control group and treatment group were injected intraperitoneally with diazepine, and the blank group were injected intraperitoneally with 0.9% sodium chloride solution for 14 days. On the 15th day, the control group was given 0.036mg/m-L risperidone suspension by gastric perfusion, the treatment group was given 0.92g/m-L Yuyang decoction, the blank group, and the model group were given corresponding volume distilled water for 14 days. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH), catalase (CAT) (CAT) and the expression of DA in prefrontal brain tissue of rats were measured. Results: the activities of SOD,GSH and CAT in prefrontal tissue in the treatment group were significantly higher than those in the model group (P0.05), while the expression of DA in the treatment group was significantly lower than that in the model group (P0.05). Conclusion: Yuwei decoction can inhibit the formation of free radicals and block the oxidative stress reaction by promoting the activity of SOD,GSH and CAT in brain tissue and transforming superoxide into non-toxic water. Thus reverse the oxidative stress response induced by hyperfunction of DA neurons.
【作者单位】：黑龙江神志医院;黑龙江中医药大学;
【基金】：国家自然科学基金项目(No.81373776,No.81473444) 黑龙江省应用技术研究与开发计划项目(No.GZ16C001) 中国博士后科学基金项目(No.2016M601467) 黑龙江省博士后项目(No.LBH-Z16180) 黑龙江省中医药中青年科技攻关项目(No.ZQG-075) 黑龙江中医药大学优秀创新人才支持计划资助项目(优秀青年教师)~~
【分类号】：R285.5;R-332

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