连续性静脉-静脉血液滤过治疗山羊挤压综合征模型的建立
发布时间：2019-03-26 17:12
【摘要】：目的建立山羊挤压综合征(CS)动物模型,并探讨连续性静脉-静脉血液滤过(CVVH)治疗对肾功能及肾组织病理变化的影响。方法 12只山羊随机分为对照组、CS模型组、CVVH治疗组,每组4只。CS模型组与CVVH治疗组山羊于后肢肌肉注射50%甘油生理盐水溶液10 mL/kg建立CS模型(对照组注射等量生理盐水),1、2、8、12、24h后检测血清肌酸激酶(sCK)和血肌酐(sCr),以sCK1 000U/L,同时sCr2倍对照值判断为造模成功。造模1h后,CVVH治疗组于造模对侧股静脉留置单针双腔导管建立血管通路,采用智能化床旁肾脏替代治疗机进行CVVH治疗。血流量为100mL/min,置换液流速为35mL/(kg·h),以前稀释法输入,治疗23h后(第24h)处死动物,留取肾组织标本,光镜、电镜下观察肾组织病理组织结构变化;采用免疫组化染色检查caspase12蛋白表达变化及TUNEL染色对细胞凋亡进行检测。结果造模山羊均于注射甘油后1h内出现酱油色小便,尿量较对照组明显减少。CS模型组与CVVH治疗组造模1h后血清sCr及sCK较对照组升高,差异有统计学意义(P0.05),提示造模成功。行CVVH治疗23h(第24h),sCK、sCr水平低于CS模型组(P0.05)。光镜下CS模型组肾组织可见急性肾小管坏死表现,肾间质水肿,肾小球基本正常。电镜下模型组可见小管上皮细胞明显的染色质聚集,线粒体肿胀,内质网扩张等早期细胞凋亡征象,CVVH治疗组表现稍轻。CS模型组及CVVH治疗组caspase12表达高于对照组(P0.001),CVVH治疗组caspase12表达低于CS模型组(P0.05)。TUNEL染色证实CS模型组山羊肾组织细胞凋亡比例明显增高,CVVH治疗组较模型组凋亡比例少。结论通过肌肉注射50%甘油生理盐水溶液可建立山羊CS模型,早期行CVVH治疗可减缓肾功能恶化,减轻肾小管上皮细胞凋亡程度。
[Abstract]:Objective To establish an animal model of goat crush syndrome (CS) and to explore the effect of continuous veno-venous hemofiltration (CVVH) on the pathological changes of renal and renal tissues. Methods 12 goats were randomly divided into control group, CS model group and CVVH group. CS model group and CVVH treatment group were used to establish CS model (control group to inject the same amount of normal saline),1,2,8,12 and 24 hours, and the serum creatine kinase (sCK) and blood myoglobin (sCr) were detected, and the sCK1 000 U/ L and sCr2 times of the control value were determined as the success of the model. After the model was set for 1 h, the CVVH treatment group was used to set up the vascular access with a single-needle double-lumen catheter in the contralateral femoral vein of the model, and the CVVH was treated with an intelligent bedside renal replacement therapy machine. The blood flow was 100 mL/ min, the flow rate of the replacement solution was 35 mL/ (kg 路 h), and the blood flow was input by the previous dilution method. After the treatment for 23 h (day 24), the animal was sacrificed, and the renal tissue specimen, the light microscope and the electron microscope were used to observe the structural changes of the renal tissue. The expression of caspase-12 and the TUNEL staining were used to detect the apoptosis of the cells. Results The amount of urine in soy sauce appeared in the model goat after injection of glycerol for 1 h, and the urine volume was significantly reduced in the control group. The serum sCr and sCK in the CS model group and the CVVH group were higher than that in the control group, and the difference was significant (P0.05). The levels of sCK and sCr were lower than that of CS model group (P0.05). In the light microscope, the renal tissue of the CS model group showed acute tubular necrosis, renal interstitial edema, and normal glomerulus. In the model group under the electron microscope, the apoptosis of the early cells such as the aggregation of chromatin, the swelling of mitochondria and the expansion of the endoplasmic reticulum were observed. The expression of caspase12 in the CS model group and the CVVH group was higher than that in the control group (P0.001), and the expression of caspase12 in the CVVH group was lower than that in the CS model group (P0.05). Conclusion The model of goat CS can be established by intramuscular injection of 50% glycerin physiological saline solution, and the early stage CVVH treatment can slow the deterioration of renal function and reduce the apoptosis of renal tubular epithelial cells.
【作者单位】： 四川大学华西医院肾脏内科;
【基金】：国家自然科学基金(No.81270818)资助
【分类号】：R642;R-332
本文编号：2447752
[Abstract]:Objective To establish an animal model of goat crush syndrome (CS) and to explore the effect of continuous veno-venous hemofiltration (CVVH) on the pathological changes of renal and renal tissues. Methods 12 goats were randomly divided into control group, CS model group and CVVH group. CS model group and CVVH treatment group were used to establish CS model (control group to inject the same amount of normal saline),1,2,8,12 and 24 hours, and the serum creatine kinase (sCK) and blood myoglobin (sCr) were detected, and the sCK1 000 U/ L and sCr2 times of the control value were determined as the success of the model. After the model was set for 1 h, the CVVH treatment group was used to set up the vascular access with a single-needle double-lumen catheter in the contralateral femoral vein of the model, and the CVVH was treated with an intelligent bedside renal replacement therapy machine. The blood flow was 100 mL/ min, the flow rate of the replacement solution was 35 mL/ (kg 路 h), and the blood flow was input by the previous dilution method. After the treatment for 23 h (day 24), the animal was sacrificed, and the renal tissue specimen, the light microscope and the electron microscope were used to observe the structural changes of the renal tissue. The expression of caspase-12 and the TUNEL staining were used to detect the apoptosis of the cells. Results The amount of urine in soy sauce appeared in the model goat after injection of glycerol for 1 h, and the urine volume was significantly reduced in the control group. The serum sCr and sCK in the CS model group and the CVVH group were higher than that in the control group, and the difference was significant (P0.05). The levels of sCK and sCr were lower than that of CS model group (P0.05). In the light microscope, the renal tissue of the CS model group showed acute tubular necrosis, renal interstitial edema, and normal glomerulus. In the model group under the electron microscope, the apoptosis of the early cells such as the aggregation of chromatin, the swelling of mitochondria and the expansion of the endoplasmic reticulum were observed. The expression of caspase12 in the CS model group and the CVVH group was higher than that in the control group (P0.001), and the expression of caspase12 in the CVVH group was lower than that in the CS model group (P0.05). Conclusion The model of goat CS can be established by intramuscular injection of 50% glycerin physiological saline solution, and the early stage CVVH treatment can slow the deterioration of renal function and reduce the apoptosis of renal tubular epithelial cells.
【作者单位】： 四川大学华西医院肾脏内科;
【基金】：国家自然科学基金(No.81270818)资助
【分类号】：R642;R-332
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