力生长因子促进骨髓间充质干细胞向成骨细胞的分化

发布时间：2019-03-27 15:11

【摘要】：背景:力生长因子具有激活肌肉卫星细胞和促进肌原细胞生长的作用,同时在维持骨质量和修复骨损伤方面具有作用。目的:研究力生长因子促进兔骨髓间充质干细胞向成骨细胞分化的作用机制。方法:采用MTT法检测力生长因子促进兔骨髓间充质干细胞成骨分化的最佳质量浓度与时间。用定量PCR和Western blot检测此质量浓度力生长因子促进兔骨髓间充质干细胞成骨分化后细胞中成骨细胞标志物碱性磷酸酶和骨钙素mRNA和蛋白的表达水平变化,并用Western Blot检测AKT和mTOR磷酸化水平变化。结果与结论:力生长因子的质量浓度为45μg/L时作用5 d促进兔骨髓间充质干细胞成骨分化的效果最好。45μg/L力生长因子促进兔骨髓间充质干细胞成骨分化后细胞中碱性磷酸酶和骨钙素mRNA和蛋白水平在干预后4 h最高,同时AKT和mTOR的磷酸化水平也在干预后4 h时最高。说明力生长因子促进兔骨髓间充质干细胞向成骨细胞的分化的作用机制是通过PI3K/AKT途径实现的,且在45μg/L干预后4 h时的效果最好。
[Abstract]:BACKGROUND: The force growth factor has the function of activating the muscle satellite cells and promoting the growth of the myogenic cells, and has the function of maintaining the bone quality and repairing the bone injury. Objective: To study the mechanism of force growth factor to promote the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts. Methods: The optimal mass concentration and time of bone marrow mesenchymal stem cells in rabbit bone marrow mesenchymal stem cells were measured by MTT assay. The expression of bone marrow mesenchymal stem cells in rabbit bone marrow-derived mesenchymal stem cells was detected by quantitative PCR and Western blot. The expression of bone marrow mesenchymal stem cells in rabbit bone marrow mesenchymal stem cells was changed, and the levels of AKT and mTOR phosphorylation were detected by Western Blot. Results and Conclusion: When the mass concentration of the force growth factor is 45. m u.g/ L, the effect of the function of 5 days to promote the osteogenic differentiation of the bone marrow mesenchymal stem cells of the rabbit is the best.45. mu. g/ L of the force growth factor promotes the development of the bone marrow mesenchymal stem cells in the rabbit, and the alkaline phosphatase and the bone-derived mRNA and the protein level in the cells are the highest after the intervention, At the same time, the phosphorylation of AKT and mTOR was also the highest at 4 h after intervention. The mechanism of the effect of force growth factor to promote the differentiation of bone marrow-derived mesenchymal stem cells into osteoblasts was achieved through the PI3K/ AKT pathway, and the effect was best at 4 h after 45. m u.g/ L intervention.
【作者单位】：内蒙古医科大学第二附属医院;重庆医科大学第一附属医院;
【分类号】：R329.2

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