小鼠胚胎呼吸内胚层相关第二生心区的发育
发布时间：2019-04-08 18:37
【摘要】：目的探讨小鼠胚胎心流出道分隔过程中,前肠呼吸内胚层与咽前第二生心区细胞发育的形态学关系及机制。方法胚龄9~13d小鼠胚胎标本各6例,连续石蜡切片,用抗转录因子叉头框蛋白A2(Foxa2)、抗胰岛因子1(ISL-1)、抗patched1(Ptc1)、抗patched 2(Ptc2)、抗α-平滑肌肌动蛋白(α-SMA)及抗心肌肌球蛋白重链(MHC)抗体进行免疫组织化学及免疫荧光染色。结果胚龄9~9.5d,前肠腹侧壁ISL-1阳性内胚层局部增厚,呼吸内胚层开始发育,ISL-1阳性间充质细胞紧随其后开始出现在呼吸内胚层周围的基质中。胚龄10~11.5d,呼吸内胚层向动脉囊方向生长延伸向喉-气管沟演变,ISL-1阳性咽前间充质细胞围绕呼吸内胚层呈对称的特征性锥体形结构分布,锥体顶端突入动脉囊腔向主-肺动脉隔发育。在喉-气管沟发育过程中,总能观察到1条实心内胚层细胞索位于其腹侧顶端,Ptc1和Ptc2主要局限于发育中的喉-气管沟及实心细胞索表达,喉-气管沟及实心细胞索的内胚层则位于锥体结构的中心。胚龄12~13d,在流出道水平前肠分隔形成气管,内胚层细胞索逐渐消失,气管上皮逐渐失去Ptc1和Ptc2表达,气管腹侧的ISL-1阳性间充质细胞密度明显减低,并逐渐停止向流出道添加,动脉囊分隔完成。结论呼吸内胚层的分化发育与咽前ISL-1阳性第二生心区细胞的发育聚集密切耦联。音猬因子(SHH)信号系统在呼吸内胚层发育过程中活跃程度较高,发育中的呼吸内胚层可能作为组织中心,通过SHH信号通路诱导ISL-1阳性细胞的聚集,并通过内胚层生长延伸造成的机械牵拉力驱动ISL-1阳性细胞迁移,参与流出道正常形态发生。
[Abstract]:Aim to investigate the morphological relationship and mechanism between the endoderm of pregut respiration and the cell development of prepharyngeal second heart during the separation of cardiac outflow tract in mouse embryos. Methods six embryonic specimens of 9-day-old 13-day-old mice were randomly divided into two groups: paraffin section, anti-Foxa2, anti-islet factor-1 (ISL-1), anti-patched1 (Ptc1) and anti-patched-2 (Ptc2). Anti-伪-smooth muscle actin (伪-SMA) and anti-myosin heavy chain (MHC) antibodies were stained by immunohistochemistry and immunofluorescence. Results ISL-1-positive endoderm was thickened in ventral wall of anterior intestine at 9-9. 5 days, respiratory endoderm began to develop, and ISL-1-positive mesenchymal cells began to appear in the matrix around respiratory endoderm. At the age of 10 to 11.5 days, the respiratory endoderm grew and extended toward the larynx-trachea sulcus, and the ISL-1-positive prepharyngeal mesenchymal cells showed symmetrical, characteristic cone-shaped distribution around the respiratory endoderm. The capsular cavity of the artery protruding into the apex of the pyramidal developed into the aortic pulmonary septum. During the development of larynx-trachea sulcus, a solid endoderm cell cord was always observed at its ventral tip. The expression of Ptc1 and Ptc2 was mainly confined to the developing laryngeal-tracheal sulcus and the solid cell cord during the development of larynx-tracheobronchial sulcus. The endoderm of larynx-trachea sulcus and solid cell cord is located in the center of pyramidal structure. At the age of 12 ~ 13 days, trachea was separated from the anterior intestine at the outflow tract level, the endoderm cell cord disappeared gradually, the expression of Ptc1 and Ptc2 was gradually lost in the trachea epithelium, and the density of ISL-1 positive mesenchymal cells in the ventral trachea was significantly decreased. And gradually stop adding to the outflow tract, arterial sac separation is complete. Conclusion the differentiation and development of respiratory endoderm are closely coupled with the development and aggregation of prepharyngeal ISL-1-positive secondary heart cells. The phonic hedgehog factor (SHH) signaling system is highly active in the development of respiratory endoderm. The developing respiratory endoderm may act as a tissue center and induce the aggregation of ISL-1 positive cells through SHH signaling pathway. The migration of ISL-1-positive cells was driven by mechanical pulling force caused by endoderm growth and extension, which was involved in the normal morphogenesis of outflow tract.
【作者单位】： 山西医科大学组织学与胚胎学教研室;长春医学高等专科学校解剖学教研室;山西医学科学院神经内科;
【基金】：国家自然科学基金(30771141,31200899) 山西省自然科学基金(2015021189) 山西省优势重点学科建设经费(转化医学) 山西医科大学博士启动基金(03201410);山西医科大学331基础医学科技培植基金(201423)
【分类号】：R321.5
本文编号：2454814
[Abstract]:Aim to investigate the morphological relationship and mechanism between the endoderm of pregut respiration and the cell development of prepharyngeal second heart during the separation of cardiac outflow tract in mouse embryos. Methods six embryonic specimens of 9-day-old 13-day-old mice were randomly divided into two groups: paraffin section, anti-Foxa2, anti-islet factor-1 (ISL-1), anti-patched1 (Ptc1) and anti-patched-2 (Ptc2). Anti-伪-smooth muscle actin (伪-SMA) and anti-myosin heavy chain (MHC) antibodies were stained by immunohistochemistry and immunofluorescence. Results ISL-1-positive endoderm was thickened in ventral wall of anterior intestine at 9-9. 5 days, respiratory endoderm began to develop, and ISL-1-positive mesenchymal cells began to appear in the matrix around respiratory endoderm. At the age of 10 to 11.5 days, the respiratory endoderm grew and extended toward the larynx-trachea sulcus, and the ISL-1-positive prepharyngeal mesenchymal cells showed symmetrical, characteristic cone-shaped distribution around the respiratory endoderm. The capsular cavity of the artery protruding into the apex of the pyramidal developed into the aortic pulmonary septum. During the development of larynx-trachea sulcus, a solid endoderm cell cord was always observed at its ventral tip. The expression of Ptc1 and Ptc2 was mainly confined to the developing laryngeal-tracheal sulcus and the solid cell cord during the development of larynx-tracheobronchial sulcus. The endoderm of larynx-trachea sulcus and solid cell cord is located in the center of pyramidal structure. At the age of 12 ~ 13 days, trachea was separated from the anterior intestine at the outflow tract level, the endoderm cell cord disappeared gradually, the expression of Ptc1 and Ptc2 was gradually lost in the trachea epithelium, and the density of ISL-1 positive mesenchymal cells in the ventral trachea was significantly decreased. And gradually stop adding to the outflow tract, arterial sac separation is complete. Conclusion the differentiation and development of respiratory endoderm are closely coupled with the development and aggregation of prepharyngeal ISL-1-positive secondary heart cells. The phonic hedgehog factor (SHH) signaling system is highly active in the development of respiratory endoderm. The developing respiratory endoderm may act as a tissue center and induce the aggregation of ISL-1 positive cells through SHH signaling pathway. The migration of ISL-1-positive cells was driven by mechanical pulling force caused by endoderm growth and extension, which was involved in the normal morphogenesis of outflow tract.
【作者单位】： 山西医科大学组织学与胚胎学教研室;长春医学高等专科学校解剖学教研室;山西医学科学院神经内科;
【基金】：国家自然科学基金(30771141,31200899) 山西省自然科学基金(2015021189) 山西省优势重点学科建设经费(转化医学) 山西医科大学博士启动基金(03201410);山西医科大学331基础医学科技培植基金(201423)
【分类号】：R321.5
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