污水厂产超广谱β内酰胺酶大肠杆菌通过接合水平传递耐药性
发布时间：2019-04-12 11:59
【摘要】：【目的】研究废水中产超广谱β-内酰胺酶大肠杆菌中可移动质粒在耐药基因水平传播机制中的作用。【方法】对污水厂分离所得的50株产ESBLs大肠杆菌进行接合试验,并对所得的接合子采用纸片扩散法测定其对15种常见药物的耐药表型,针对质粒介导的产ESBLs菌株的耐药基因设计7对特异性引物对接合子进行PCR扩增。【结果】研究结果显示,80份水样分离得50株产ESBLs大肠杆菌,共接合成功35株细菌,接合成功率高达70%。接合子与供体菌相比,均发生耐药谱型的改变,且存在丢失一种或几种药物耐药性且产生另一种或几种药物耐药性的现象。PCR扩增结果显示,接合子与供体菌相比,耐药基因型有所减少或不变,bla_(TEM)、bla_(CTX-M)基因全部接合成功,bla_(SHV)基因仅1株未接合成功,耐氟喹诺酮类基因未发生转移。【结论】本研究表明,不同的耐药基因可能位于不同的可移动质粒上,可移动质粒在大肠杆菌耐药性水平传播的过程中起到了十分重要的作用。
[Abstract]:[objective] to study the role of moveable plasmid in extended-spectrum 尾-lactamase-producing Escherichia coli in the horizontal transmission mechanism of drug-resistant genes in wastewater. [methods] 50 strains of ESBLs-producing Escherichia coli isolated from sewage plants were used for conjugation test. The resistance phenotype to 15 common drugs was determined by disk diffusion method. Seven pairs of specific primers were designed to amplify the resistant gene of plasmid-mediated ESBLs-producing strain by PCR. [results] the results showed that the resistant phenotype to 15 kinds of common drugs was detected by PCR. [results] the results of this study showed that the resistant phenotype to 15 common drugs was amplified by PCR with 7 pairs of primers. Fifty strains of ESBLs-producing Escherichia coli were isolated from 80 water samples. 35 strains of E. coli were successfully conjugated, and the successful rate of conjugation was up to 70%. Compared with donor bacteria, the heterozygotes showed changes in drug resistance patterns, and the loss of one or more drug resistance and the production of another or more drug resistance. PCR results showed that compared with donor bacteria, the heterozygote had the same drug resistance as donor bacteria, and the results of PCR amplification showed that compared with donor bacteria, the heterozygote had one or more drug resistance. The genotype of drug resistance was decreased or unchanged, the bla_ (TEM), bla_ (CTX-M) gene was successfully joined, the bla_ (SHV) gene was not successfully joined, and the fluoroquinolone resistant gene was not transferred. [conclusion] the present study indicates that the gene of fluoroquinolone is not transferred. Different drug resistance genes may be located on different moveable plasmids, which play a very important role in the level of drug resistance transmission in E. coli.
【作者单位】： 山东农业大学动物科技学院;山东省农业科学院;泰山医学院基础医学部;
【基金】：基金项目:山东省农业产业技术创新体系家禽创新团队(SDA/T-11-09)~~
【分类号】：R378
，
本文编号：2456991
[Abstract]:[objective] to study the role of moveable plasmid in extended-spectrum 尾-lactamase-producing Escherichia coli in the horizontal transmission mechanism of drug-resistant genes in wastewater. [methods] 50 strains of ESBLs-producing Escherichia coli isolated from sewage plants were used for conjugation test. The resistance phenotype to 15 common drugs was determined by disk diffusion method. Seven pairs of specific primers were designed to amplify the resistant gene of plasmid-mediated ESBLs-producing strain by PCR. [results] the results showed that the resistant phenotype to 15 kinds of common drugs was detected by PCR. [results] the results of this study showed that the resistant phenotype to 15 common drugs was amplified by PCR with 7 pairs of primers. Fifty strains of ESBLs-producing Escherichia coli were isolated from 80 water samples. 35 strains of E. coli were successfully conjugated, and the successful rate of conjugation was up to 70%. Compared with donor bacteria, the heterozygotes showed changes in drug resistance patterns, and the loss of one or more drug resistance and the production of another or more drug resistance. PCR results showed that compared with donor bacteria, the heterozygote had the same drug resistance as donor bacteria, and the results of PCR amplification showed that compared with donor bacteria, the heterozygote had one or more drug resistance. The genotype of drug resistance was decreased or unchanged, the bla_ (TEM), bla_ (CTX-M) gene was successfully joined, the bla_ (SHV) gene was not successfully joined, and the fluoroquinolone resistant gene was not transferred. [conclusion] the present study indicates that the gene of fluoroquinolone is not transferred. Different drug resistance genes may be located on different moveable plasmids, which play a very important role in the level of drug resistance transmission in E. coli.
【作者单位】： 山东农业大学动物科技学院;山东省农业科学院;泰山医学院基础医学部;
【基金】：基金项目:山东省农业产业技术创新体系家禽创新团队(SDA/T-11-09)~~
【分类号】：R378
，
本文编号：2456991
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