转铁蛋白受体1对小鼠海马神经元生长与分化的影响

发布时间：2019-05-21 11:21

【摘要】：目的探讨转铁蛋白受体1(TfR1)对小鼠海马神经元生长与分化的影响。方法取胚胎18.5 d小鼠原代海马神经元培养,培养7 d后使用TfR1 shRNA p GFP-V-RS干扰质粒转染小鼠海马原代神经元使TfR1基因沉默,采用绿色荧光蛋白(GFP)分析及Western blotting技术检测TfR1 shRNA的转染效率;4’6-二脒基-2-苯基吲哚(DAPI)及TUNEL法检测干扰后海马神经细胞凋亡情况,免疫荧光技术检测凋亡后神经细胞骨架的变化及神经元突起生长的形态特征。结果 TfR1 shRNA质粒能有效使神经细胞内TfR1基因沉默;TfR1基因沉默后,原代海马神经细胞凋亡率增加,细胞骨架部分崩解,且神经元树突突起长度明显增长(P0.05)。结论转铁蛋白受体1可通过调控小鼠海马神经元树突的生长来影响海马神经元的分化。
[Abstract]:Objective to investigate the effect of transferrin receptor 1 (TfR1) on the growth and differentiation of hippocampal neurons in mice. Methods the primary hippocampal neurons of mice were cultured at 18.5 days of embryo. After 7 days of culture, the primary hippocampal neurons of mice were transformed with TfR1 shRNA p GFP-V-RS interference plasmid to silence the TfR1 gene. Green fluorescent protein (GFP) analysis and Western blotting technique were used to detect the transfection efficiency of TfR1 shRNA. 4 the apoptosis of hippocampal neurons after interference was detected by (DAPI) and TUNEL, and the changes of neuronal skeleton and the morphological characteristics of neuronal processes after apoptosis were detected by immunofluorescence technique. Results TfR1 shRNA plasmid could effectively silence TfR1 gene in neural cells, and after TfR1 gene silencing, the apoptosis rate of primary hippocampal neurons increased, the cytoskeleton partially disintegrated, and the dendritic process length of neurons increased significantly (P 0.05). Conclusion Transferrin receptor 1 can affect the differentiation of hippocampal neurons by regulating the dendritic growth of hippocampal neurons in mice.
【作者单位】：漯河医学高等专科学校解剖学教研室;漯河医学高等专科学校分子医学实验室;
【基金】：河南省科技厅重点科技攻关项目(132102310111) 漯河医学高等专科学校基金(2013-S-LMC01)
【分类号】：R338

【相似文献】