加味四妙方治疗尿酸性肾病模型大鼠的实验研究

发布时间：2019-05-24 21:38

【摘要】：目的探讨加味四妙方对尿酸性肾病大鼠的肾功能、血脂代谢、尿液生化和肾组织病理方面的影响。方法采用酵母、腺嘌呤制作尿酸性肾病大鼠模型。大鼠随机分为正常组(ZC组)、模型组(MX组)、别嘌醇组(XY组)、加味四妙方组(SMF组),给予相应干预。检测给药后大鼠肾功能[尿素氮(BUN)、肌酐(Scr)、尿酸(UA)],血脂四项[胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)],尿液生化[N-乙酰-β-D葡萄糖苷酶(NAG),β2微球蛋白(β2-MG)]和24 h尿蛋白代谢的变化情况;观察肾组织病理改变。结果 1与正常组比较,模型组的BUN、Scr、UA、TC、TG、HDL和LDL、NAG、β2-MG和24 h尿蛋白均有显著的统计学差异(P0.01)。2与模型组比较,加味四妙方组的BUN、Scr、UA、TC、LDL、NAG、β2-MG和24 h尿蛋白代谢显著降低(P0.01);别嘌醇组的UA显著降低(P0.01),而别嘌醇组的Scr、TC、TG、HDL差异无统计学意义(P0.05)。3肾组织病理改变显示,加味四妙方组和别嘌醇组较模型组肾小管内尿酸盐结晶减少,炎性细胞浸润减少,肾小管-间质损伤较轻,其中加味四妙方组损伤最轻,与别嘌醇组比较差异有统计学意义(P0.05)。结论加味四妙方能够显著改善尿酸性肾病大鼠的肾功能、血脂代谢、尿液生化,减轻肾组织病理损害。
[Abstract]:Objective to investigate the effects of Jiawei Simiao recipe on renal function, blood lipid metabolism, urine biochemistry and renal histology in rats with uric acid nephropathy. Methods the rat model of uric acid nephropathy was established by yeast and adenine. The rats were randomly divided into normal group (ZC group), model group (MX group), allopurine group (XY group) and Jiawei Simiao recipe group (SMF group). The renal function of rats [urea nitrogen (BUN), creatine (Scr), uric acid (UA)], blood lipid four items [cholesterol (TC), triacylglycerol (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL)] were measured after administration. Changes of urine biochemical [N-acetyl尾-D-glucosidase (NAG), 尾 2 microglobulin (尾 2-MG)] and 24 h urinary protein metabolism; The pathological changes of renal tissue were observed. Results 1 compared with the normal group, there were significant differences in BUN,Scr,UA,TC,TG,HDL, LDL,NAG, 尾 2-MG and 24 h urinary protein between the model group and the model group (P 0.01). 2 compared with the model group, the BUN,Scr,UA, in the Jiawei Simiao recipe group was significantly different from that in the model group. TC,LDL,NAG, 尾 2-MG and 24 h urinary protein metabolism decreased significantly (P 0.01). UA in allopurinol group was significantly lower than that in allopurine group (P 0.01), but there was no significant difference in Scr,TC,TG,HDL in allopurinol group (P 0.05). 3 the pathological changes of renal tissue showed that, Compared with the model group, the urate crystallization, inflammatory cell infiltration and tubulostroma injury in Jiawei Simiao recipe group and allopurine group were less than those in model group, and the injury in Jiawei Simiao recipe group was the least. There was significant difference between allopurine group and allopurine group (P 0.05). Conclusion Jiawei Simiao recipe can significantly improve renal function, blood lipid metabolism, urine biochemistry and alleviate pathological damage of renal tissue in rats with uric acid nephropathy.
【作者单位】：上海市普陀区利群医院中医肾病科;
【基金】：上海市卫计委第三批中医临床优势专科建设项目(ZYSNXD-YL-YSZK024)
【分类号】：R277.5;R-332

【相似文献】